IRF1-IN-1-生命科学试剂-MCE-6527

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEIRF1-IN-1Cat.No.:HY-171006CASNo.:701225-07-2分⼦式:C₂₂H₂₄N₄O₄S分⼦量:440.52作⽤靶点:SARS-CoV;Caspase;PARP;Pyroptosis;InterleukinRelated;IFNAR作⽤通路:Anti-infection;Apoptosis;CellCycle/DNADamage;Epigenetics;Immunology/Inflammation储存⽅式:4°C,sealedstorage,awayfrommoisture*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoisture)溶解性数据体外实验DMSO:83.33mg/mL(189.16mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM2.2700mL11.3502mL22.7004mL5mM0.4540mL2.2700mL4.5401mL10mM0.2270mL1.1350mL2.2700mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoisture)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。BIOLOGICALACTIVITY⽣物活性IRF1-IN-1(CompoundI-2)⼀种IRF1抑制剂。IRF1-IN-1可减少IRF1对CASP1启动⼦的招募。IRF1-IN-1抑制细胞死亡信号通路(即抑制Caspase1、GSDMD、IL-1和PARP1的裂解)。IRF1-IN-1对电离辐射引起的⽪肤炎症性损伤有保护作⽤[1]。IC50&TargetPARP-1IL-1Caspase-11/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE体外研究IRF1-IN-1pretreatment(20μM,12ꢀh)decreasestherecruitmentofIRF1tothepromoterofCASP1inirradiatedHaCaTcells(20ꢀGy)[1].IRF1-IN-1(20μM,24h)attenuatestheNSP-10plasmidtransfection-inducedIRF1activationinHELF,HaCaTandWS1cells[1].IRF1-IN-1(50ꢀμM,24ꢀh)treatmentreducesthetranscriptionalactivityofIRF1inHELFcells96ꢀhafterSARS-CoV-2pseudovirusinfection[1].IRF1-IN-1decreasesradiation(20Gy)-inducedcelldeathinK150cells[1].IRF1-IN-1maintainsmitochondrialactivityandROSproductioninskincellsintheearlystageafterirradiation[1].IRF1-IN-1significantlyinhibitsthecelldeathsignalingpathway,i.e.,suppressescleavageofCaspase1,GSDMD,IL-1andPARP1[1].体内研究IRF1-IN-1pretreatment(100ꢀμg/d,s.c.,oneeveryotherdaybeforeirradiation)hasapotentialprotectiveeffectin35Gyradiation-inducedinflammatoryskininjurymice[1].AnimalModel:Radiogenicskininjurymice(intraperitonealinjectionofpentobarbitalsodium(1%,30mg/kg),35Gyatthedoserateof1000cGy/minbya6-MeVelectronbeam)[1]Dosage:100μg/dAdministration:Subcutaneousinjection(s.c.),oneeveryotherdaybeforeirradiation,pretreatmentResult:Showedasignificantreductioninacuteskininflammatorymanifestations,suchaserythemaandexudationandacceleratedthehealingprocess.Showedprotectiveeffectsonthefunctionandstructuralintegrityofradiation-inducedlesionstotheclaws.REFERENCES[1].Geng,etal.Chaperone-andPTM-mediatedactivationofIRF1tamesradiation-inducedcelldeathandtheinflammatoryresponse.CellMolImmunol.2024Aug;21(8):856-872.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalappl