3D类器官研究手册

目录 P09 030608091011 18 18 18 18 19 19 19 19 20 20 20 23类器官概述·家们已成功构建包括脑类、肠类、心脏、肝脏、肾脏、肺、乳腺、视网膜在内的各种正常或肿瘤类器官。类器官技术是近年来生物医学领域最具突破性的前沿技术之一，已经广泛应用于功能组织诱导、疾病模型构建、药物发现和筛选、个性化伴随诊断、细胞治疗、再高高低短低高高低高高低高高低高BioreactorBioreactorOrganoiddevelopment>GrowthfactorsApplicationsDrugscreeningGenetherapyBiobankingHumanorgandevelopment▲IdentificationSkin/blood< >人类器官研究解决方案·高品质为您提供高活性、低内毒素、高批高品质为您提供高活性、低内毒素、高批义翘神州开发了一系列重组生长因子，具有高活性、低内毒素、高批间一致性和高纯度等特点，以实现类器官的最佳生长，并确保获得重复ActivinA10429-HNAHHuman,Mouse,Rat,Cynomolgus,RhesusBioactiveNativebasicFGF/FGF2HumanBioactiveNative10609-HNAE2HumanBioactiveNative10605-HNAEHumanBioactiveNative<5EU/mgHumanBioactiveNative<5EU/mg10598-HNAEHumanBioactiveNativeHumanBioactiveN-HisNoggin/NOG10267-HNAHHumanBioactiveNativeR-Spondin1/HumanCHOStableCellsBioactiveNativeWnt3aWNT007-02HBioactiveC-hFcHPLC-VerifiedHuman/CynomolgusVEGF165mAU2050(Cat#:11066-mAU2050HumanFGF-7mAUmAU806040200(Cat#:10573-HNAE)MkDa 66.245.035.0M25.0kDa 66.245.035.025.0MkDa kDa 66.245.035.025.0MkDa 66.245.035.025.0M≥95%asdeterminedbySDS≥95%asdeterminedbySDSand≥95%asdeterminedbySDSandHumanEGF(Cat#:10605-HNAE)国外知名品牌国外知名品牌义翘神州批次1义翘神州批次2义翘神州批次2806040200-20HumanEGFConc.(pg/mL)MeasuredinacellproliferationassayusingBalb/C3T3mouseembryonicfibroblasts.(Cat#:11083-HNAS)3000000国外知名品牌义翘神州300000025000002000000150000010000005000000Conc.(ng/mL)Measuredbyitsabilitytoinduceactivationofß-cateninresponseinaTopflashLuciferaseassayusingHEK293Tcells.HumanHGF(Cat#:10463-HNAS)500义翘神州500国外知名品牌Proliferation,%400Proliferation,%3002000HGF(ng/mL)MeasuredbyitsabilitytoneutralizeTGF-betamediatedinhibitiononMv-1-Lucellproliferation.HumanEGF(Cat#:10605-HNAE)Batch1Batch2Batch1Batch2Batch3Proliferation,%Proliferation,%806040200-20HumanEGF(ng/mL)ThreeindependentbatchesofhumanEGFprotein(Cat#:10605-HNAE)haveidenticalbioactivity.(Cat#:11083-HNAS)3000000Batch1Batch230000002500000Batch325000002000000150000010000005000000Conc.(ng/mL)ThreeindependentbatchesofhumanRSPO1protein(Cat#:11083-HNAS)haveidenticalbioactivity.HumanHGF(Cat#:10463-HNAS)500Batch1 Batch2Proliferation,%Batch3400Proliferation,%Batch33002000HGF(ng/mL)ThreeindependentbatchesofhumanHGFprotein(Cat#:10463-HNAS)haveidenticalbioactivity.HumanEGF(Cat#:10605-HNAE)BCP7day7BCThyroidorganoidDay1Day5Day15Enlargement(>3months)ThyroidorganoidThyroidorganoidsThyroidorganoidsHumanRSPO1(Cat#:11083-HNAS)BCRCCRC-CRC-Day1Day3Day7cLM-P2OM-P6CRC-P10CRC-P14TissueTissueOrganoidOrganoidHumanRSPO1,MouseNOG,andMouseEGF(11083-HNAS/50688-M02H/50482-MNCH)APatient1Patient2Patient3Day0Day24BDay0Day24Patient4Patient5Patient6；（MouseFGF-7(Cat#:50394-M07E)(10014-HNAE/11066-HNAH(10014-HNAE/11066-HNAH/10605-HNAE)whole100pixelSuprabasal100pixeliPSC-derivedhumanvascularorganoidswereculturedwithFGF2(Cat#:iPSC-derivedhumanvascularorganoidswereculturedwithFGF2(Cat#:10014-HNAE),VEGF165(Cat#:11066-HNAH),EGF(Cat#:10605-HNAE).Redarrowsrepresentvascularorganoids.类器官标志物有助于确认类器官是否成功建立以及反映细胞生理病理变化等情况。义翘神州为客户提供一系列高质量的类器官标志物抗体，适用于免疫组化、免疫荧光、流式、WB和免(Cat#:100130-MM19)ImmunochemicalstainingofhumanKi67inhumanlungcancer.E-cadherin抗体(Cat#:10204-R032)ImmunochemicalstainingofhumanE-cadherininhumangastriccancer.p63抗体当(Cat#:100266-R143)Immunochemicalstainingofhumanp63inhumanbreastcarcinoma.Cytokeratin8抗体(Cat#:108772-MM05)ImmunochemicalstainingofhumanCytokeratin8inhumanprostate.SOX-2抗体(Cat#:100106-R017)ImmunochemicalstainingofhumanSOX2inhumantestis.(Cat#:10694-R028)ImmunofluorescencestainingofHumanEpCAMinSKBR3cells.(Cat#:100129-R101)ImmunochemicalstainingofhumanCK14inhumanesophagealcarcinoma.(Cat#:100306-MM08)ImmunochemicalstainingofhumanKRT19inhumanthyroidcancer.(Cat#:202163-T42)ImmunofluorescencestainingofKRT20inHeLacells.细胞凋亡是细胞受到特定基因调控后产生的主动性死亡过程。凋亡检测可用于评估类器官在接受各种信号刺激后，细胞凋亡的发生情况。义翘神州提供两款AnnexinV/7-AAD凋亡检测试剂盒（Cat#:APK10448-F；Cat#:APK10448-P）用于活细胞比例检测。与国外品牌对比，FITC/PEfluorescentConjugatesFITC/PEfluorescentConjugates PhosphatidylserineNucleus7-AAD+EarlyapoptosisAnnexinV+7-AAD-NormalcellsAnnexinV-7-AAD- LateapoptosisAnnexinV+7-AAD/PIAnnexinVNucleusNucleusCaCaCaCa7-ADD 7-ADD AnnexinV-Alexa488AnnexinV-FITC7-ADD7-ADD义翘神州 7-ADD7-ADD义翘神州 2 AnnexinV-FITCAnnexinV-FITC AnnexinV/7-AADApoptosisDetectionKits(Cat#:APK10448-F)S/N=89S/N=89S/N=93S/N=93AnnexinV/7-AADApoptosisDetectionKitsS/N=65S/N=50S/N=39正常组织类器官·10605-HNAEHumanNative<5EU/mgGMP-10605-HNAEeoHumanNative<5EU/mgHumanNativeHumanNativeHumanNative10267-HNAHHumanNative10804-HNACHuman,Rhesus,Cynomolgus,CanineNativeHumanNativeShhHumanC-His50240-M08HMouseC-HisHumanNativeHumanNativeHumanC-His10609-HNAE2HumanNativeHumanN-HisHumanNativeHumanNativeHumanRabbitPAbHumanRabbitMAbIHC-PHumanMouseMAbIHC-P101696-T46HumanRabbitPAb205715-T40HumanRabbitPAbWB,IHC-PNeuropilin-210695-MM01HumanMouseMAbTransthyretin201630-T10HumanRabbitPAbIHC-P,ICC/IF100568-T08HumanRabbitPAbIHC-PNCadherinHumanRabbitMAbICC/IF203658-T08HumanRabbitPAbIHC-P13498-MM01THumanMouseMAbICC/IFHPLC-VerifiedGMPGrade10605-HNAEHumanNative<5EU/mgGMP-10605-HNAEeHumanNative<5EU/mgHumanNative13084-H02H1HumanC-hFc10267-HNAHHumanNativeHumanNative<5EU/mgHumanNativeHumanNativeWnt-3aWNT007-02HC-hFcHumanN-HisGMP-10210-HNAE-LeoHumanNativeHumanNativeHumanN-cleavageHumanRabbitPAbHumanRabbitMAbp63100266-R143HumanRabbitMAbIHC-PCytokeratin8108772-MM05HumanMouseMAbIHC-P,ICC/IF10694-R028HumanRabbitMAbE-cadherin10204-R032HumanRabbitMAbIHC-P,ICC/IFActinHumanRabbitPAbWB,IHC-PHPLC-VerifiedGMPGrade人乳腺癌类器官RSPO1(11083-HNAS),NOG(50688-M0EGF(50482-M01H/50482-MNCH)10605-HNAEHumanNative<5EU/mgGMP-10605-HNAEeHumanNative<5EU/mg10267-HNAHHumanNativeHumanNativeHumanNativeHumanNativeHumanN-HisGMP-10210-HNAE-LeoHumanNativeHumanNative<5EU/mgActivinA10429-HNAHHuman,Mouse,Rat,Cynomolgus,RhesusNativeActivinAGMP-10429-HNAHeoHuman,Mouse,Rat,Cynomolgus,RhesusNative10609-HNAE2HumanNative102305-T02HumanRabbitPAbICC/IFUteroglobinMouseRabbitMAbIHC-PE-cadherin10204-R032HumanRabbitMAbIHC-P,ICC/IFHumanRabbitPAbp63100266-R143HumanRabbitMAbIHC-PHumanRabbitMAbIHC-PCytokeratin8108772-MM05HumanMouseMAbIHC-P,ICC/IFCytokeratin17201453-T10HumanRabbitPAbIHC-P,ICC/IFHPLC-VerifiedGMPGrade人肺癌类器官FGF4(16043-HNAE),NOG(50688-M02H),EGF(50482-M01H/50482-MNCH)FGF4(16043-HNAE),NOG(50688-M02H),EGF(50482-M01H/50482-MNCH)HumanNativeHumanNative16043-HNAEHumanNativeHumanNative<5EU/mg10609-HNAE2HumanNative14890-HNAHHumanNativeActivinA10429-HNAHHuman,Mouse,Rat,Cynomolgus,RhesusNativeActivinAGMP-10429-HNAHCOHuman,Mouse,Rat,Cynomolgus,RhesusNative110984-T02HumanRabbitPAbICC/IFHumanMouseMAbIHC-P107330-T08MouseRabbitPAbIHC-PNCadherinHumanRabbitMAbICC/IFbeta-CateninHumanRabbitMAbICC/IFHumanMouseMAbAlpha-fetoproteinHumanMouseMAbICC/IFVE-Cadherin10433-MM01HumanMouseMAbTroponinI100758-T08HumanRabbitPAbIHC-PHumanNativeHumanNativeHuman,CynomolgusNative10605-HNAEHumanNative<5EU/mgGMP-10605-HNAECOHumanNative<5EU/mg10609-HNAE2HumanNativeWnt-3aWNT007-02HC-hFcHumanMouseMAb90215-T24RhesusRabbitPAb100125-T40HumanRabbitPAbWB,IHC-PActinHumanRabbitPAbWB,IHC-PHumanMouseMAbIHC-PVE-Cadherin10433-MM01HumanMouseMAbHPLC-VerifiedGMPGrade10605-HNAEHumanNative<5EU/mgGMP-10605-HNAEeoHumanNative<5EU/mg10267-HNAHHumanNativeHumanNativeHumanNativeHumanNative16043-HNAEHumanNativeActivinA10429-HNAHHuman,Mouse,Rat,Cynomolgus,RhesusNativeActivinAGMP-10429-HNAHCOHuman,Mouse,Rat,Cynomolgus,RhesusNative10609-HNAE2HumanNativeHumanNative13059-HNAEHumanNativeWnt-3aWNT007-02HC-hFcAlbuminHumanRabbitMAb204167-T46HumanRabbitPAb100687-T04HumanRabbitPAbCD34HumanMouseMAbIHC-Pc-KitHumanRabbitMAb10694-R028HumanRabbitMAbHumanRabbitPAbE-cadherin10204-R032HumanRabbitMAbIHC-P,ICC/IFChromograninA100256-MM02HumanMouseMAbIHC-P202163-T42HumanRabbitPAbHumanMouseMAbIHC-PHumanRabbitMAbIHC-PShhHumanC-HisWnt-3aWNT007-02HC-hFc200388-T34HumanRabbitPAbHumanMouseMAbIHC-PHumanRabbitMAbIHC-PNestinHumanMouseMAb206278-T44HumanRabbitPAbVimentin100254-R001HumanRabbitMAbWB,IHC-PHPLC-VerifiedGMPGrade10605-HNAEHumanNative<5EU/mgGMP-10605-HNAEeoHumanNative<5EU/mg10267-HNAHHumanNativeHumanNativeHumanNativeHumanNativeHumanNative<5EU/mgActivinA10429-HNAHHuman,Mouse,Rat,Cynomolgus,RhesusNativeActivinAGMP-10429-HNAHCOHuman,Mouse,Rat,Cynomolgus,RhesusNative16043-HNAEHumanNative10609-HNAE2HumanNative10463-HNASHumanNativeHumanNativeHumanNativeBMP-2Human,Mouse,Rat,Rhesus,CanineNativeHumanC-His10804-HNACHuman,Rhesus,Cynomolgus,CanineNative10452-HNAHHumanNativeGMP-10452-HNAHeoHumanNative<5EU/mgWnt-3aWNT007-02HC-hFcHumanN-HisHumanNativeHumanNative100306-MM08HumanMouseMAbIHC-P201893-T42HumanRabbitPAbAlbuminHumanRabbitMAb10694-R028HumanRabbitMAb102305-T02HumanRabbitPAbICC/IFE-cadherin10204-R032HumanRabbitMAbIHC-P,ICC/IF204407-T44HumanRabbitPAbABCC2203123-T08HumanRabbitPAbIHC-PHPLC-VerifiedGMPGrade10605-HNAEHumanNative<5EU/mgGMP-10605-HNAEeoHumanNative<5EU/mg10267-HNAHHumanNativeHumanNativeHumanNative<5EU/mgHumanNative10609-HNAE2HumanNativeHumanRabbitPAbIHC-P,ICC/IFThyroidperoxidaseHumanMouseMAbELISA(Cap),IHC-P202914-T08HumanRabbitPAbIHC-P200239-T32HumanRabbitPAb102305-T02HumanRabbitPAbICC/IF标志物抗体10605-HNAEHumanNative<5EU/mgGMP-10605-HNAECOHumanNative<5EU/mg10267-HNAHHumanNativeHumanNativeHumanNative<5EU/mgHumanNativeActivinA10429-HNAHHuman,Mouse,Rat,Cynomolgus,RhesusNativeActivinAGMP-10429-HNAHeoHuman,Mouse,Rat,Cynomolgus,RhesusNative10609-HNAE2HumanNativeWnt-3aWNT007-02HC-hFcHumanRabbitPAbIHC-PHumanRabbitPAbICC/IF104604-T36HumanRabbitPAb204167-T46HumanRabbitPAbHumanRabbitMAbIHC-PE-cadherin10204-R032HumanRabbitMAbIHC-P,ICC/IF100125-T40HumanRabbitPAbWB,IHC-PHumanRabbitPAb12072-MM07HumanMouseMAbHPLC-VerifiedGMPGrade10605-HNAEHumanNative<5EU/mgGMP-10605-HNAEeoHumanNative<5EU/mg10267-HNAHHumanNativeHumanNativeActivinA10429-HNAHHuman,Mouse,Rat,Cynomolgus,RhesusNativeActivinAGMP-10429-HNAHCOHuman,Mouse,Rat,Cynomolgus,RhesusNativeHumanNative<5EU/mgHumanNativeHumanNativeHumanRabbitMAbCytokeratin8108772-MM05HumanMouseMAbIHC-P,ICC/IFp63100266-R143HumanRabbitMAbIHC-PHumanMouseMAbIHC-PAndrogenreceptor207175-T08HumanRabbitPAbIHC-PHumanRabbitMAbE-cadherin10204-R032HumanRabbitMAbIHC-P,ICC/IFVimentin100254-R001HumanRabbitMAbWB,IHC-P100573-T08HumanRabbitPAbIHC-PHumanRabbitPAbChromograninA100256-MM02HumanMouseMAbIHC-P10605-HNAEHumanNative<5EU/mgGMP-10605-HNAEeoHumanNative<5EU/mg10267-HNAHHumanNativeHumanNativeHumanNative<5EU/mgHumanNativeHumanNativeWnt-3aWNT007-02HC-hFc100418-T36HumanRabbitPAb204167-T46HumanRabbitPAb104590-T10HumanRabbitPAbIHC-P,ICC/IF100306-MM08HumanMouseMAbIHC-PHPLC-VerifiedGMPGradeBMP-2Human,Mouse,Rat,Rhesus,CanineNative10609-HNAE2HumanNativeHumanNativeHumanNative16043-HNAEHumanNativeHumanN-HisHumanNativeHumanNative10463-HNASHumanNative101696-T46HumanRabbitPAb100588-T08HumanRabbitPAbIHC-PNCadherinHumanRabbitMAbAquaporin1202650-T08HumanRabbitPAbIHC-PE-cadherin10204-R032HumanRabbitMAbIHC-P,ICC/IFTIM-170001-R202CanineRabbitMAbVimentin100254-R001HumanRabbitMAbWB,IHC-PHumanMouseMAbHumanRabbitPAbIHC-P,ICC/IF标志物抗体10605-HNAEHumanNative<5EU/mgGMP-10605-HNAEeoHumanNative<5EU/mgHumanNativeHumanNative<50EU/mgHumanNative10609-HNAE2HumanNativeHumanNativeHumanNativeHumanRabbitPAbIHC-P,ICC/IFE-cadherin10204-R032HumanRabbitMAbIHC-P,ICC/IF101696-T46HumanRabbitPAbHumanRabbitMAbIHC-PHumanRabbitPAbJagged1MouseRabbitPAbIHC-P,ICC/IFHumanRabbitPAbHumanRabbitPAb207078-T10HumanRabbitPAbIHC-P,ICC/IF203531-T44HumanRabbitPAb10046-MM06HumanMouseMAbHPLC-VerifiedGMPGrade肿瘤类器官·10694-R028HumanRabbitMAbAlpha-fetoproteinHumanMouseMAbICC/IF100393-T02HumanRabbitPAbICC/IFbeta-CateninHumanRabbitMAbHumanMouseMAbIHC-P100306-MM08HumanMouseMAbIHC-PHumanRabbitMAb100310-MM02HumanMouseMAbIHC-P202163-T42HumanRabbitPAbHumanMouseMAbIHC-PE-cadherin10204-R032HumanRabbitMAbIHC-P,ICC/IFp5390001-R002CynomolgusRabbitMAbIHC-P,ICC/IF100310-MM02HumanMouseMAbIHC-P202163-T42HumanRabbitPAbHumanMouseMAbIHC-PHumanRabbitMAbCytokeratin8108772-MM05HumanMouseMAbIHC-P,ICC/IFp5390001-R002CynomolgusRabbitMAbIHC-P,ICC/IFHumanRabbitMAb200169-T46HumanRabbitPAbp63100266-R143HumanRabbitMAbIHC-PCD44HumanRabbitPAb100310-MM02HumanMouseMAbIHC-PHumanMouseMAbp5390001-R002CynomolgusRabbitMAbIHC-P,ICC/IF100306-MM08HumanMouseMAbIHC-P10694-R028HumanRabbitMAbHumanRabbitMAbIHC-P,ICC/IFN-CadherinHumanRabbitMAbHumanRabbitMAbHumanMouseMAbIHC-P108990-T08MouseRabbitPAbIHC-PCD44HumanRabbitPAbHumanRabbitPAbIHC-P,ICC/IFNapsinAHumanRabbitPAbIHC-P100310-MM02HumanMouseMAbIHC-Pp63100266-R143HumanRabbitMAbIHC-PHumanRabbitMAbSynaptophysin100298-R249HumanRabbitMAbIHC-PERalpha100386-T10HumanRabbitPAbIHC-P,ICC/IF100362-T08HumanRabbitPAbIHC-PHer210004-R002AHumanRabbitMAbHumanMouseMAbIHC-PHumanRabbitMAbp63100266-R143HumanRabbitMAbIHC-PHumanRabbitMAbCytokeratin8108772-MM05HumanMouseMAbIHC-P,ICC/IFP-cadherinMouseRabbitPAbIHC-PE-cadherin10204-R032HumanRabbitMAbIHC-P,ICC/IFHumanRabbitPAb100973-R035HumanRabbitMAbIHC-P,ICC/IFHumanMouseMAbIHC-PHumanRabbitPAb202163-T42HumanRabbitPAbbeta-CateninHumanRabbitMAbP-CK100675-T36HumanRabbitPAbHumanMouseMAbHumanMouseMAbIHC-P100729-T08HumanRabbitPAbIHC-P100272-MM05HumanMouseMAbICC/IF100573-T08HumanRabbitPAbIHC-Pp63100266-R143HumanRabbitMAbIHC-PHumanMouseMAbIHC-P100926-T38HumanRabbitPAb100125-T40HumanRabbitPAbWB,IHC-PHumanRabbitMAbCytokeratin8108772-MM05HumanMouseMAbIHC-P,ICC/IFE-cadherin10204-R032HumanRabbitMAbIHC-P,ICC/IFVimentin100254-R001HumanRabbitMAbWB,IHC-PHumanRabbitMAbp5390001-R002CynomolgusRabbitMAbIHC-P,ICC/IFHumanRabbitPAbIHC-P,ICC/IFCytokeratin8108772-MM05HumanMouseMAbIHC-P,ICC/IFERalpha100386-T10HumanRabbitPAbIHC-P,ICC/IF12609-RP02HumanRabbitPAb100362-T08HumanRabbitPAbIHC-P100310-MM02HumanMouseMAbIHC-PE-cadherin10204-R032HumanRabbitMAbIHC-P,ICC/IFHer210004-R002AHumanRabbitMAb202163-T42HumanRabbitPAbHumanMouseMAbIHC-PCD9HumanMouseMAb100306-MM08HumanMouseMAbIHC-P10694-R028HumanRabbitMAb11388-MM03HumanMouseMAbCD44HumanMouseMAbSynaptophysin100298-R249HumanRabbitMAbIHC-PHumanMouseMAbIHC-PChromograninA100256-MM02HumanMouseMAbIHC-P100306-MM08HumanMouseMAbIHC-PHumanMouseMAb热门产品推荐·10426-HNAEHuman,Mouse,CanineRat脑Human脑50025-MNAEMouseHumanHumanHuman57248-M08HMouse10605-H01HHuman50482-MNAYMouseYeast50482-M01HMouse50482-MNCHMouse50037-M07EMouse50394-M07EMouseHuman脑HumanHumanHuman脑50038-MNAHMouse80429-RNAHRat10598-HNAEHuman50437-MNAYMouseYeast13059-HNAEHumanHumanYeast50756-MNAHMouse14890-H08HHuman50385-MNACMouseCHOStableCells50688-M02HMouse10267-H02HHuman10267-H08HHuman10658-H01HHumanHuman10