20222分子生物学试题 A

第第页20222分子生物学试题A卷号：〔A〕〔2022年12月〕绝密

湖北师范学院一般本科期末考试试卷

分子生物学

考试范围考试形式

闭卷

全部章节课程类别

命题人

必修学期

王友如20222

系部生命科学学院专业生物技术

一、名词说明〔翻译并简约说明，每题2分，共16分〕

1.tRNAi2.RNAi3.intein4.intron5.codingregion;6.codingstrand;7.operon8.operator;

二、单项选择题(选择正确答案，每题1.5分，共24分)

9.WhichenzymedoesnotplayaroleinDNAreplication?()

(A)DNApolymerase(B)Helicase

(C)Single-strandedbindingprotein(D)Guanyltransferase10.Splicingistheprocessthatdoeswhichofthefollowing?()

(A)Removeintronsandconservee*ons(B)Removee*onsandconserveintrons

(C)RemovemutatedregionsofprimarytranscriptRNA

(D)AddmultipleadenosinebasestotheendofaprimaryRNAtranscript11.The5'cappingprocesscreateswhattypeoflinkage?()

(A)5'-3'(B)3'-5'(C)5'-5'(D)3'-3'

12.Codonsthatcodeforthesameaminoacidarecalled:()

(A)Synonyms(B)Similar(C)Degenerates(D)Complements

13.Whichvectorwouldyouchoosetoe*pressaforeigngeneinaplant?D

Aabaculovirusvector.Baretroviralvector.CaYepvector.DaT-DNAvector.14.Whatsignalstheendoftranscription?()

(A)Stopcodon(B)Terminator(C)TheendoftheDNAchain(D)RNApolymeraserunsout

15.Whatisthenameofthemoleculethatisaddedtothe5'endofeukaryoticRNAtranscripts?()

(A)ATP(B)SnRNP(C)GTP(D)Phosphate

16.Accordingtothewobblehypothesis,aUinthe5'positionoftheanitcodoncanpairwith:()

(A)UorC(B)AorG(C)A,C,orG(D)OnlyA

17.Whichtwoofthefollowingstatementsabouttranscriptionfactorsaretrue?D

Atheheli*-turn-heli*domainisatranscriptionalactivationdomain.Bdimerizationoftranscriptionfactorsoccursthroughthebasicdomain.CleucinezippersbindtoDNA.

DitisoftenpossibletogetfunctionaltranscriptionfactorswhenDNAbindingdomainsandactivationdomainsfromseparatetranscriptionfactorsarefusedtogether.

18.Whichofthefollowingisnotcharacteristicofprokaryoticterminators?()

(A)Rho-independentterminators(B)Rho-dependentterminators(C)TheTATAbo*(D)Hairpinloops

19.WhatistheenzymeresponsibleforstitchingtogetherOkazakifragments?()

(A)Helicase(B)Single-strandedbindingprotein(C)Primase(D)Ligase

20.DenaturationofdoublestrandedDNAinvolves.Apreakageintoshortdouble-strandedfragments.Bseparationintosinglestrands.ChydrolysisoftheDNAbackbone.

Dcleavageofthebasesfromthesugar-phosphatebackbone.

21.Inprokaryotes,thelaggingstrandprimersareremovedby...A3'to5'e*onuclease.BDNAligase.

CDNApolymeraseI.DDNApolymeraseIII.

22.Prokaryoticplasmidscanreplicateinyeastcellsiftheycontainaclonedyeast..

AORC.BCDK.CARS.DRNA.

23.Pernucleotideincorporated,thespontaneousmutationfrequencyinE.coliis...

A1in106.B1in108.

C1in109.D1in1010.

24.Whichtwoofthefollowingstatementsaboutattenuationatthetrpoperonaretrue?Aattenuationisrho-dependent.

Bdeletionoftheattenuatorsequenceresultsinanincreaseinbothbasalandactivatedlevelsoftranscriptionfromthetrppromoter.

Ctheattenuatorliesupstreamofthetrpoperatorsequence.

Dattenuationdoesnotrequiretightcouplingbetweentranscriptionandtranslation.

Epausingofaribosomeattwotryptophancodonsintheleaderpeptidewhentryptophanisinshortsupplycausesattenuation.

25.Whichoneofthefollowingstatementsaboutcatabolite-regulatedoperonsisfalse?AcAMPreceptorprotein(CRP)andcataboliteactivatorprotein(CAP)aredifferentnamesforthesameprotein.

BwhenglucoseispresentinthecellcAMPlevelsfall.CCRPbindstocAMPandasaresultactivatestranscription.DCRPbindstoDNAintheabsenceofcAMP.

ECRPcanbendDNA,resultinginactivationoftranscription.

三、填空题(每空1分，共10分)

26.Thesimplestcloningvectoratleastcontainsandorielements.27.ThediagrambelowshowstwoRNAmolecules.MoleculeIisprocessed

togivemoleculeII.Answerthefollowingquestionsaboutthesetwomolecules:Wheredoesthisconversionhappen?____________________________WhattypeofRNAmoleculeismoleculeI?_________________________WhattypeofRNAmoleculeismoleculeII?_________________________Nametheregions/structuresindicatedbythearrows:A=_________________B=_______________

C=_________________D=______________E=________

四、简答题〔回答要点,每题4分，共五小题，共20分〕

28WhenyoustudytheDNAcomponentsofphageΦΧ174,yougettheresultsasfollows:A，25%；T，33%；G，24%；C，18%.Question(1):Doesyourresultsabidebybasepairingrules?Question(2):Howdoyoue*plainyourresults?

29.DNAligaseplaysimportantroleintheprocessofDNAreplication,butligasedoesnotfunctionintheprocessofRNAsynthesis.Couldyoue*plainit?30DoyouthinkthatrRNAandtRNAaremorestablethanmRNA?Why?31.HowdoesacodingregionDNAsequencecodedifferenttypesofprotein?32.Couldyoulist4kindsofRNAthathavetheabilitytocatalyticactivity?

五、问答题〔每题10分，共20分〕。

33.PleasetellusthetypesofRNAthatyouknow,itwouldbebetterthatyoue*plaintheirfunctioninlivingorganisms.(10points)

34.Takethecaseofarabinoseoperon,Couldyoue*plainwhatcis-actingelementandtrans-actingfactorare,howdotheyinteractwitheachothertoregulatethisoperon?(10points)

六、分析题〔每题10分，共10分〕35.AscientistgotagalpromotermutantE.coli.whichcannotusegalactose(半乳糖)inthemedium,ifhemutatedthegenecya(geneofcAMP环化酶)andcrp(cAMPAcceptorProteingene)weremutatedintocya-andcrp-respectively,thesecondmutatedE.coli.restoreditsabilitytousegalactose.Couldyoue*plaintheresultsbyusingyourmolecularbiologicalknowledge?

卷号：〔A〕〔2022年12月〕绝密

湖北师范学院一般本科期末考试试卷

分子生物学

考试范围考试形式

闭卷

全部章节课程类别

命题人

必修学期

王友如20222

系部生命科学学院专业生物技术

一、名词说明〔翻译并简约说明，每题2分，共16分〕

1.tRNAi2.RNAi3.intein4.intron5.codingregion;6.codingstrand;7.operon8.operator;

二、单项选择题(选择正确答案，每题1.5分，共24分)

9.WhichenzymedoesnotplayaroleinDNAreplication?()

(A)DNApolymerase(B)Helicase

(C)Single-strandedbindingprotein(D)Guanyltransferase10.Splicingistheprocessthatdoeswhichofthefollowing?()

(A)Removeintronsandconservee*ons(B)Removee*onsandconserveintrons

(C)RemovemutatedregionsofprimarytranscriptRNA

(D)AddmultipleadenosinebasestotheendofaprimaryRNAtranscript11.The5'cappingprocesscreateswhattypeoflinkage?()

(A)5'-3'(B)3'-5'(C)5'-5'(D)3'-3'

12.Codonsthatcodeforthesameaminoacidarecalled:()

(A)Synonyms(B)Similar(C)Degenerates(D)Complements

13.Whichvectorwouldyouchoosetoe*pressaforeigngeneinaplant?D

Aabaculovirusvector.Baretroviralvector.CaYepvector.DaT-DNAvector.14.Whatsignalstheendoftranscription?()

(A)Stopcodon(B)Terminator(C)TheendoftheDNAchain(D)RNApolymeraserunsout

15.Whatisthenameofthemoleculethatisaddedtothe5'endofeukaryoticRNAtranscripts?()

(A)ATP(B)SnRNP(C)GTP(D)Phosphate

16.Accordingtothewobblehypothesis,aUinthe5'positionoftheanitcodoncanpairwith:()

(A)UorC(B)AorG(C)A,C,orG(D)OnlyA

17.Whichtwoofthefollowingstatementsabouttranscriptionfactorsaretrue?D

Atheheli*-turn-heli*domainisatranscriptionalactivationdomain.Bdimerizationoftranscriptionfactorsoccursthroughthebasicdomain.CleucinezippersbindtoDNA.

DitisoftenpossibletogetfunctionaltranscriptionfactorswhenDNAbindingdomainsandactivationdomainsfromseparatetranscriptionfactorsarefusedtogether.

18.Whichofthefollowingisnotcharacteristicofprokaryoticterminators?()

(A)Rho-independentterminators(B)Rho-dependentterminators(C)TheTATAbo*(D)Hairpinloops

19.WhatistheenzymeresponsibleforstitchingtogetherOkazakifragments?()

(A)Helicase(B)Single-strandedbindingprotein(C)Primase(D)Ligase

20.DenaturationofdoublestrandedDNAinvolves.Apreakageintoshortdouble-strandedfragments.Bseparationintosinglestrands.ChydrolysisoftheDNAbackbone.

Dcleavageofthebasesfromthesugar-phosphatebackbone.

21.Inprokaryotes,thelaggingstrandprimersareremovedby...A3'to5'e*onuclease.BDNAligase.

CDNApolymeraseI.DDNApolymeraseIII.

22.Prokaryoticplasmidscanreplicateinyeastcellsiftheycontainaclonedyeast..

AORC.BCDK.CARS.DRNA.

23.Pernucleotideincorporated,thespontaneousmutationfrequencyinE.coliis...

A1in106.B1in108.

C1in109.D1in1010.

24.Whichtwoofthefollowingstatementsaboutattenuationatthetrpoperonaretrue?Aattenuationisrho-dependent.

Bdeletionoftheattenuatorsequenceresultsinanincreaseinbothbasalandactivatedlevelsoftranscriptionfromthetrppromoter.

Ctheattenuatorliesupstreamofthetrpoperatorsequence.

Dattenuationdoesnotrequiretightcouplingbetweentranscription