人抗胰岛素IgG检测HumanAntiInsulinIgGELISAKit说明书

第第页人抗胰岛素IgG检测HumanAntiInsulinIgGELISAKit说明书人抗胰岛素IgG检测试剂盒HumanAntiInsulinIgGELISAKitcat3600HIGINTENDEDUSEADIsAntiInsulinELISATestKithasbeendesignedforthequantitativedeterminationofAutoantibodiestoHumanInsulininserumandplasma.TheassayisusefulforthedeterminationofTypeIDiabetes,aswellasforscreeningpurposestodetectdevelopinginsulinautoantibodiesinmonkeyunderinsulintherapy.INTRODUCTIONTypeIDiabetesismainlycharacterizedbylimitedorfullymissingsecretionofthehormoneinsulin.MorphologicalstudiesdemonstratedadestructionofthebetacellsofthesocalledLangerhannssCells（IsletCells）inTypeIdiabetics.Numerousresearchersdescribedtheappearanceofantibodiesdirectedagainsttheisletcellsandinsulinasthecausalreasonfortheonsetofthedisease.AntiInsulinantibodiesarefoundin37percentofpatientswithnewlydetectedTypeIDiabetes,in4%oftheirrelativesofthefirstdegreeandinupto1.5%ofhealthycontrols.ApositivecorrelationbetweentheappearanceofantiInsulinandantiIsletCellantibodieshasbeenreported.AntiInsulinautoantibodiesmaybedetectedseveralmonthsandinsomecasesyearsbeforetheonsetofthefullyclinicalmanifestationofthediseases.OccasionallyalsoautoantibodiestoProInsulinmayappear.ThesetrueantiInsulinautoantibodiesdirectedagainstendogenousinsulinhavetobedistinguishedfromthoseautoantibodieswhicharedevelopedininsulindependentdiabeticsundergoingtherapywithinsulinpreparationsofanimalorigin.Infactthelatterhavetobereferredtosideeffects.Thesesideeffectsmayoccuraslocalreactionsoftheskinbydevelopmentofinsulinspecificautoantibodies.Theseautoantibodiesarecausingtheformationofaninsulindepotandtheymaysimulatearesistanceagainstthehormonaltreatmentwithanimalinsulin.SPECIFICITYandAnimalCrossreactivityThemicroplateiscoatedwithhighlypurifiedpreparationsofrecombinanthumaninsulin.TheconjugateusedinthekitisagainstthehumanIgG,therefore,onlyIgGsubclassantibodieswillbedetected.ThiskitwillnotdetectantiinsulinIgGinotherspecies.ADIhasseparateantiinsulinELISAkitsformonkey,mouseandratsamples.StandardUnitCalibrationNointernationalreferencepreparationforhumanantiInsulinautoantibodiesisavailable,theassaysystemiscalibratedinrelativearbitraryunits.References:Jin,X（2023）Exp.Biol.Med.235,877885；KoulmandaM（2023）Am.J.Transplant.3,267272；TheriaultBR（1999）Transplantation.68:3317Wagner,JD（2023）Toxicol.Pathol.29,142148；Reeves,W.G.etal（1983）Diabetologia,24,399；Atkinson,M.A.,（1985）Diabetes34,926930；Willein,T.（