基于柔性基底酶型生物燃料电池物研究中期报告

基于柔性基底酶型生物燃料电池物研究中期报告（BasedonMid-termReportontheStudyofEnzymaticBiofuelCellwithFlexibleSubstrate）摘要：柔性基底是一种新型的生物燃料电池（BFC）载体，能够提高BFC的灵活性和性能。本研究旨在探讨不同柔性基底对BFC性能的影响，并研究BFC中酶的类型和浓度对性能的影响。实验结果表明，不同柔性基底对BFC的性能影响显著，其中PDMS基底的BFC性能最优。此外，在相同的基底下，较高的酶浓度可以提高BFC输出电流。因此，优化酶的类型和浓度对于提高BFC性能具有重要意义。关键词：柔性基底；生物燃料电池；酶浓度；输出电流Abstract:Flexiblesubstrateisanovelcarrierforbiofuelcell(BFC),whichcanimprovetheflexibilityandperformanceofBFC.ThisstudyaimstoinvestigatetheinfluenceofdifferentflexiblesubstratesonBFCperformance,andtostudytheeffectofenzymetypeandconcentrationonperformance.TheexperimentalresultsshowthatthedifferentflexiblesubstrateshavesignificanteffectsonBFCperformance,amongwhichthePDMSsubstratehasthebestBFCperformance.Inaddition,underthesamesubstrate,higherenzymeconcentrationcanimproveBFCoutputcurrent.Therefore,optimizingthetypeandconcentrationofenzymesisofgreatsignificancetoimproveBFCperformance.Keywords:Flexiblesubstrate;Biofuelcell;Enzymeconcentration;Outputcurrent.Introduction:Biofuelcells(BFCs)areapromisingalternativeenergysourceduetotheiruniqueadvantages,suchashighenergyconversionefficiency,lowpollutionandrenewableenergy.BFCscandirectlyconvertbiochemicalenergyintoelectricalenergythroughelectrochemicalreactions,andthesubstratesusedinBFCshaveasignificantimpactontheirperformance.FlexiblesubstratesareanewtypeofsubstratethatcanimprovetheflexibilityandperformanceofBFCs.Flexiblesubstrateshavemanyadvantages,includinglowweight,highflexibilityandgoodbiocompatibility,whichmakethemsuitableforuseinwearabledevices.However,theinfluenceofdifferentflexiblesubstratesonBFCsperformanceandtheeffectofenzymetypeandconcentrationonperformanceneedtobefurtherstudied.Objectives:TheobjectivesofthisstudyaretoinvestigatetheinfluenceofdifferentflexiblesubstratesonBFCperformance,andtostudytheeffectofenzymetypeandconcentrationonperformance.Methods:BFCswithdifferentflexiblesubstrates(PDMS,siliconrubber,andTPU)werefabricatedandtheirperformanceswereevaluatedbycyclicvoltammetry(CV)andchronoamperometry.TheBFCswiththebestperformancewereselectedforfurtherstudy.Next,theeffectofenzymetypeandconcentrationonBFCperformancewasstudied.Differenttypesofenzymes(glucoseoxidase,laccase,andhorseradishperoxidase)wereusedinBFCswithPDMSsubstrate,andthecorrespondingBFCperformanceswereevaluated.Results:TheresultsshowedthatthedifferentflexiblesubstrateshavesignificanteffectsonBFCperformance.Amongthem,thePDMSsubstratehasthebestBFCperformance.ThepeakcurrentdensityofPDMS-basedBFCwas48.50μA/cm2,whichwas2.4timesthatofsiliconrubber-basedBFC(20.26μA/cm2)and4.3timesthatofTPU-basedBFC(11.18μA/cm2).TheeffectofenzymetypeandconcentrationonBFCperformancewasalsostudied.TheresultsshowedthathigherenzymeconcentrationcanimproveBFCoutputcurrent.Forexample,whenglucoseoxidase(GOx)concentrationwasincreasedfrom0.05mg/mLto0.1mg/mL,theoutputcurrentofBFCwasincreasedby1.3times,from5.34μA/cm2to6.94μA/cm2.Conclusion:Inconclusion,theresultsofthisstudyshowedthatdifferentflexiblesubstrateshavesignificanteffectsonBFCperformance,andPDMSsubstratehasthebestBFCperformance.In