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/生物医药与海洋开发1、福建地道药材泽泻种质复壮与种苗繁育技术TheTechnologyofSeedQualityRejuvenationandSeedlingBreedingofFujianGenuineMedicineMaterialAlismaorientali种子质量是发展泽泻的瓶颈,泽泻种子寿命短,常温下1年几乎丧失发芽能力,且种质退化严重,植株生长与药材质量参差不齐产量低下.经种质复壮种子发芽率不低于78%、矮缩株不低于30%、千粒重不低于0。22g;繁育种苗38—45天苗高8—18cm、无病斑、无虫蛀、心芽完整、展开真叶保持有5片以上为合格种苗,实现稳产丰产,药材质量达到国家药典标准。ThequalityofAlismaorientaliseedisthekeyrestrictivefactor.Theseedhasashortlife-span,almostlosesthegerminatingabilityinroomtemperatureinoneyear。Germplasmdegeneratesseriously,theplantgrowesirregularly,andtheyieldandqualityofAlismaorientaliislow。Withseedqualityrejuvenation,thegerminationpercentageisnotlessthan78%,thedwarfspercentagearenotlessthan30%,thethousandgrainweightisnotlessthan0。22g。With38-45daysofbreeding,thequalifiedseedlingiswiththeheightof8-18cm,integratedbud,andmorethan5trueleafs,withoutdiseaseandpestspeckle.Thus,theyieldofAlismaorientaliishighandsteady,andthequalityofAlismaorientalimedicinalmaterialsaccordswiththestandardofnationpharmacopoeia。联系单位:福建省农科院农业生物资源研究所联系人:陈菁瑛电话:0传真:72、鱼腥草病虫害生态防治及其标准技术操作规程TheEcologicalControlMeasureandSOPofChineseHerbalHouttuyniaecordata鱼腥草是一种具有多种药用功能的中药材,以生产注射剂为主,根茎叶还可作为蔬菜食用,应用广泛,但在种植过程容易遭受多种病虫为害,造成不同程度损失。生态防治建立严格防病的种茎基地、杜绝初侵染源,采用微生物制剂、捕食螨等生态防治技术既避免农药、重金属污染又可达到净化环境目的,满足生产GAP药材的要求。ThechineseherbalHouttuyniaecordatahasmanymedicinalapplications,themainisinjectionmedicine,anditalsoisusedasvegetable.undertheconditionofman_madeprotectiveculture,theyieldandqualityofHouttuyniaecordatawillbelostbecauseofthevariouspestsanddiseases。TherhizomatousseedbasesofHouttuyniaecordata,whichestablishedbyEcologicalcontrolmeasure,willstoptheprimaryinsectionsourcesofdiseasesandpests.Ecologicalcontrolmeasuresuchasmicrobialpreparationandpredatorymiteswillaviodthepesticideandheavymetalpollution,alsocleanupenvironment,whichsatisfiestherequirementofmedicinalmaterialsGAP.联系单位:福建省农科院农业生物资源研究所联系人:陈菁瑛电话:0传真:73、ISCOMs技术在海水鱼病害免疫防治中应用UsingISCOMstechnictocontroldiseaseofoceanicfish。获国家发明专利的ISCOMs制备技术已用于水产病原菌疫苗和抗病原的功能性卵黄蛋白制备。若用于海水鱼的防治,将减少发病率30%以上。TheISCOMstechnologyhasbeenappliedinmanufactureofvaccineandfunctionalyelkprotein。Thistechnologymayreduce30%incidenceofseafishdisease.联系单位:福建省农业科学院生物技术研究所联系人:龚晖电话:4传真:64、复合酶与膜技术在木立芦荟全叶液汁生产中的应用示范heApplicationonTheTechnologyofComplexEnzymeTreatmentandMembraneinProductionofAloeJuice本研究率先采用复合酶处理和膜过滤等技术,有效解决木立芦荟液汁易沉淀、易褐变的技术难题,开发出高稳定性、高纯度、高有效成分的木立芦荟全叶液汁。主要技术指标:提取率>60%;芦荟甙(mg/100ml)>5;产品澄清透明,保质期〉12个月;蛋白质(mg/100g):30-50;可溶性固体物质(%):1-3。各项产品指标均符合国家相关标准,实现年产木立芦荟全叶液汁300吨.Thestudyappliedthetechnologyofcomplexenzymetreatmentandmembranefilteringtosolvedaloejuiceprecipitateandbrowning.What’smore,thestudyexploitatedtheaolejuiceofhighstabilitypurityandeffectivecomponent.Themainspecificationsofproductionisthatextractionrate>60%;aloin(mg/100ml)>5;thejuicetransparent,EXP〉12monthsprotein(mg/100g):30-50;solublesolid(%):1—3.Allspecificationsconsistwithrelatedstandardsofnation.Aloejiuceproduced300toneveryyear.联系单位:福建师范大学生命科学学院联系人:何文锦电话:传真:05、珍稀贝类西施舌异地育苗产业化TheNurseryIndustrializationofRareShellfishXishiTongueatLianjiang福建师范大学拥有《西施舌规模化人工育苗技术》科研成果,省科技厅鉴定达到国际领先水平。拟在连江县异地人工育苗产业化,年增殖种苗300万粒,放流海域200亩,放流增殖将产西施舌商品蚌1000kg,年增加产值800万元,恢复连江海域西施舌自然资源。FujianNormalUniversitywiththeresearchachievementnamed"thescaleartificialbreedingtechnologyofXishitongueCoelomactraantiquata",willmakethetechnologytransfertoputtheindustrializationofnurseryandbreedingatLianjiang.Theprojectaimstopromotetheproliferationof3millionseedlingsyearly,dischargewaters200acres.Theproliferationdischargewillbe1000kgofgoodsand8millionyuaninoutputvaluewillbeincreased.Lianjiangwillrestorenaturalresourcesoftheclam.联系单位:福建师范大学联系人:高如承电话:8传真:06、激光动力鼻咽癌内镜定位仪ThediagnosisandlocalizationofNPCwithThelaserinducedfluorescence世界上80%鼻咽癌发生在我国南方各省,鼻咽癌的早期诊断与治疗是提高鼻咽癌患者生存率的关键。本仪器采用现代光电技术和高灵敏度探测器件,包括双波长氩离子激光器、像增强器、高速计算机图像采集与处理系统、光纤导光器件与技术。通过对肿瘤药物荧光成像实现对早期鼻咽癌的高灵敏度诊断与精确定位.本项成果可拓展于其它肿瘤的早期诊断与定位。Nasopharyngealcarcinoma(NPC)isauniquemalignantcancerwithdistinctgeographicfeaturesfromotherheadandneckepithelialmalignancies.TherearegeographicregionsintheworldwhereNPCisendemic,suchasinSoutheastAsia,ornorthernAfrica,wheretheannualincidencecanbeupto1/4000.AnovelopticalbiopsysystembasedonthelaserinducedfluorescenceforthediagnosisandlocalizationofearlyNPChasbeensuccessfuldeveloped,whichcoupledtheexcitationlightintotheoriginalwhite-lighttransmissionsystemofcommercialnasalendoscopeandcharacterizedbycoaxialimagingforboththefluorescenceimageandtwilightimagefromthesametargets.Thefluorescenceandreflectionwhitelightfromthetargettissuesurfacearealternatelyimagedbyanasalendoscope,whichcanbeswitchedinrealtimeforclinicalexaminationofdiagnosisandlocalizationofearlyNPC.联系单位:福建师范大学联系人:林宏心电话:2传真:37、结核病早期特异诊断试剂盒Earlyspecificdiagnosiskitformycobacteriumtuberculosis结核是我国危害最大的传染病之一,结核患者及时准确的早期诊断是结核规范性治疗的起点。本项目利用基因工程技术和细胞免疫学技术研制出结核特异性T细胞免疫体外检测方法—-T细胞IFN-γ体外释放试验(IGRA),对活动性结核的检测灵敏度和特异性均在90%以上。Tuberculosis(TB)isoneofthemostharmfulinfectiousdiseaseinChina.TheearlypathogenicspecificdiagnosisthepreconditionforTBcontrol.Inthisproject,aTBspecificIFN-γreleaseassaywasdeveloped.ThesensitivityandspecificityoftheassayforactiveTBdiagnosiswerehigherthan90%。单位单位:厦门大学国家传染病诊断试剂与疫苗工程技术研究中心联系人:张军电话:传真:8、中医舌诊辅助中成药使用系统heAssistantSystemontheUseofChinesePatentDrugsbyTongue-inspectionofTraditionalChineseMedicine中医舌诊辅助中成药使用系统是一套基于中医专家经验和现代计算机技术的软硬件系统。它能指导中成药的正确使用,可广泛用于药店、诊所、保健中心.TheAssistantSystemontheUseofChinesePatentDrugsbyTongue-inspectionofTraditionalChineseMedicineisasetofsoftwareandhardwaresystembasedontheexperienceoftraditionalChinesemedicinespecialistandmoderncomputertechnology.ItcanguidethecorrectuseofChinesepatentdrugs,andbeappliedinthedrugstores,clinicsandhealthcarecentres.单位单位:厦门大学联系人:王彦晖电话:传真:9、新型生物杀线虫药PL菌剂的中试与推广ExperimentationandApplicationofbiologicalnematicide”Paecilomyceslilacinu”.创制了高杀线虫活性的PL菌剂,每克粉剂活孢子5×108以上,每克原粉1010以上,比国外高5倍;线虫减少80%以上,促生增产15%以上;解决了货架寿命难题;原料低廉,取得2项发明专利授权;已应用在大豆、番茄等,同时可抑制柑橘黄化,示范2万棵.无三废排放无毒无害无污染;成为禁用化学杀线虫药的替代产品,已列入国家星火计划项目。PLpreparationhadexcellentnematocidaleffectwasbecreated.Itscontenthigher5timesthanoverseas,preponderatedover5×108livespores/grampowderpreparation,and〉1010livespores/gramoriginalpowder.Nematodesdecreased>80%,yieldincrease>15%,andshelflifedifficutproblemhasbeenresolved。Rawmaterialpriceischeapand2inventionpatentwarranthadbeenobtained.Ithasbeenappliedforgreatareaincitrus,tomatoandsoybean,andhasbeingdemonstratedfor20thousandsofcitrustrees.Ithavenotanytoxi,harmandpollute。Thereforebecomesubstituteofforbitedchemicalnematocides,andrankedonNationalStarandTorchProgramofChina.单位单位:厦门大学联系人:潘沧桑电话:传真:0592-218062410、海洋生物毒素标样研制及其检测新技术Massproductionofparalyticshellfishtoxinsandnoveldetectionmethods本项目将分离纯化中国沿海典型麻痹性贝毒C1/C2,GTX1/4,GTX2/3和GTX5,运用液相色谱-质谱联用技术和核磁共振等方法定量毒素纯度和含量,制备高纯度毒素标样,建立麻痹性贝毒的大规模生物生产技术;合成筛选适宜的毒素化学传感器-冠状醚,优化毒素和冠状醚的反应体系,结合多重时间分辨荧光技术,建立麻痹性贝毒多重时间分辨荧光检测技术,并用于水产品中麻痹性贝毒的检测。Thisprojectwillmassproducehighpurityparalyticshellfishtoxins(PSTs)usingacombinationofphotobioreactor,HPLC—MSandNMRapproaches,anddevelopnovelPSTsdetectionmethodbasedonfluorescentcharacteristicsofcrownethers,andfinallyapplythisproductandmethodforPSTsmonitoringinseafoods.单位单位:厦门大学联系人:王大志电话:传真:11、富集硒酵母生产ProductionofConcentratingSeleniumIonsofYeast缺硒容易产生机体抗病能力下降、肝炎、糖尿病、癌症等多种疾病。北京大学的科研人员修饰了微生物的基因获得优势菌株,在微生物发育中将硒元素与其蛋白质结合,形成与人体自身结构类似的生物态硒产品,具有毒性低,吸收快等优点,达到国际先进水平,可取代无机硒及人工合成的有机化合物等低端产品。Creaturesareeasytogetsicknessiftheylackselenium.Researchersobtainedadvantagestrainsbymodifiedmicrobialgenes。Byusedthestrains,seleniumelementcancombinedwithproteinstogettheselenium-enrichedbiologicalproduct.Ithaslowtoxicityandiseasytoabsorptionbycreatures.It

mayreplacetheselowqualitySeproduces,forexampleinorganicseleniumorsyntheticorganicseleniumcompounde.Thistechnologyisappraisedtohavereachedinternationaladvancedlevel.单位单位:北京大学联系人:罗大珍电话:010-62755498传真:010-6275549812、用于慢粒基因诊断的纳米基因传感器的研制Developmentofnano-genicbiosensorsofChronicMyelocyticLeukemiaDNA本项目相关研究是在国家863计划,国家自然科学基金,福建省重大项目及福建省产业技术开发项目的资助下进行的。本项目将具有灵敏、简便、准确、经济等优点DNA电化学生物传感器用于慢粒白血病(CML)的早期诊断.结合分子杂交技术、纳米技术、电化学检测技术,通过CML相关基因的分子自组装等固定化技术研究、高灵敏度杂交指示剂的筛选及其与DNA的作用机理等研究,建立高灵敏度、高特异性、简便经济的CML基因检测新方法,成功研制了CML的早期诊断的电化学传感器样机,有望推广应用于其他类型白血病及肿瘤的早期诊断和筛选抗肿瘤新药等生命科学领域,将对肿瘤等疾病诊断领域产生巨大的经济社会效益。FujianMedicalUniversitywasfoundedin1937。Throughthedevelopmentofthepast70years,theschoolhasestablishedtalentstrainingsystemtocultivantebachelors,mastersanddoctorsandformedthefineschoolspiritwhichwas“diligence,preciseness,practicality,innovation”tobecomeanintegratedprovincialMedicalUniversitywhichstraddlesseveraldisciplines:teaching,scientificresearch,medicaltreatment,preventionandsocialservices。In2003,theschoolwasgradedasoneofkeybuildinguniversitiesbytheFujianProvincialPeople'sGovernment.单位单位:福建医科大学联系人:张艳玲电话:4传真:413、型中药脱毛、脱皮暨除尘装置anewtypeequipmentfordepilatingandshedingofsomeChineseherbs,andalsocompaningwithashhandling因部分中药材如泽泻、莪术等球茎表面带有粗皮或粗毛,无法直接使用,需经多道烦琐、耗能、耗时的加工。本成果采用变速电机带动内面粗糙的圆形滚筒对此类药材进行加工,达到脱毛、脱皮的目的;同时,附属的除尘机构可以有效地实施除尘,防止污染环境.是一种高效、环保、节能新装置.SomeChineseherbalmedicineshaveroughsurfacewithcoarsewoolsuchasZexie,Ezhubulbs,whichcouldnotbedirectlyusedformedicine.Theyneedseveralprocessingstepswithcumbersome,energy,time-consuming.Thisachievementintroducethevariablespeedmotor—driventocarrythecirculardrumwithroughinternalsurfacetoreachsheding,peelingpurpose;Atthesametime,thesubsidiarydustremovalattachmentcanbeeffectivelyimplementedtopreventpollutionoftheenvironment.Thisachievementisahighlyefficient,environmentalfriendly,energy-savingnewdevice.联系单位:福建中医学院联系人:吴水生电话:传真:514、硫酸阿贝卡星注射液InjectionofArbecacineSulfate产品的主要用途:应用于因敏感菌所致的支气管炎、肺炎、肾盂肾炎、腹膜炎、膀胱炎、中耳炎、败血症等疾病人群。本产品对革兰阳性和阴性杆菌,如沙雷杆菌、大肠杆菌、变形杆菌、枸橼酸杆菌、肠杆菌属、克雷白杆菌等良好的抗菌活性;对氨基糖苷类灭活霉有高度拮抗力,对金黄色葡萄球菌(包括耐甲氧西林菌株)有良好的抗菌作用,MIC50或MIC80为0。78—1.56ug/耐,在氨基糖苷类抗生素中最强。Arbecacinesulfateisasemisyntheticantibiotichavingahighantibacterialactivityagainstdrugresistantbacteriaandhenceitiswidelyusedinclinicalapplications.联系单位:福州博立医药科技有限公司联系人:张国庆电话:传真:715、应用多酶体技术提高蜂花粉中活性蛋白提取率的中试研究Thepilotplantresearchforincreasingtherefiningrateofactivealbumeninhoneybeepollenbyadoptingenzymetechnology该项目是应用酶工程原理,采用以纤维素酶为主体的多酶体系,根据蜂花粉细胞特征,设定细胞壁酶解催化过程,使细胞壁溶解破裂,整个酶解条件温和,可最大程度释放花粉中活性蛋白和其它营养成分.该项目工艺简单、节能环保,已进行了中试研究,总结出了规范的工艺规程,可投入工业化生产。Thisproject,applyingenzymeengineeringprincipleandadoptingthecellulose—centeredmultienzymesystem,accordingtobeepollencellcharacteristic,setacourseforcellularwallenzymetodecomposeandmakecellularwalldissolved。Thewholedissolutionisgentle.Italsocanreleasetheactivealbumenandnutritioningredientinpolleninthemaximumextent.Thetechnicsofthisprojectissimple,Energy-savingandenvironmentalprotection,andaccomplishingthepilotplantresearch;Wehavesummarizedanormativetechnicregulationforitplungingintoindustrialproducing.联系单位:福州恒丰量子生物科技有限公司联系人:罗小梅电话:0传真:116、白血病残留病灶ALLGLO探针荧光定量PCR诊断试剂盒研发与生产ToExploreandProduceAllGloReal-timeQuantitativePCRforDiagnosingMinmalResidualDiseaseinPatientswithLeukemia此成果是一种具有中国人自主知识产权的生物高科技产品,具有高敏感性、高特异性和高检出率,达到国际先进水平。可有效地用于白血病微小残留病基因的检测,协助判断白血病的临床诊治效果和预后判断。该检测试剂盒的产业化具有广阔的市场前景,能提高福建基因诊断试剂企业在全国的影响,促进我国今基因诊断市场的快速发展.Thisachievementisakindofhigh—techbiologicalproducts,whichhasindependentintellectualpropertyrights.Italsohasmorehighersensitivity、specificityanddetectionratethanothers,andreachesadvancedinternationalstandards。Itcaneffectivelybeusedtodiagnoseminimalresidualdiseasesofleukemiagenes,andtodeterminetheeffectivenessofclinicaldiagnosisandtreatmentandtheprognosisofleukemiapatients。Itsindustrializationhasavastmarketprospect,anditcanimprovetheinfluenceofgeneticdiagnosticreagentofFujianenterprisesinourcountryandrapidlypromotethedevelopmentofgenediagnosis。联系单位:福建医科大学联系人:沈建箴电话:传真:0591—2805370017、“海保明”明目功能性食品TheFunctionalFoodforImprovingEyesight

本项目从石决明中提取有效成分,配合中医传统验方,研制成有明目等功能的海洋功能性食品,对改善用眼过度的现代人的视力状况大有裨益。本项目采用了多项先进技术以确保其工艺的先进性及制成品的质量。其中石决明水解利用技术已获得国家知识产权局的发明专利申请号.产品适合各种年龄人群的视力保健,可发挥石决明清肝明目的作用,改善眼部炎症、弱视、白内障和近视等眼病的治疗效果。Thisprogramiscontributedtoimprovingtheover—exhaustedeyesightofmodernpeoplebyextractingingredientsfromtheabaloneshellstodevelopafunctionaloceanfoodinaccordancewiththerecipesoftraditionalChinesemedicineprovedeffective.SomeAdvancedtechniqueshadbeenadoptedtoensurequalityoftheproduct。ApatentinhydrolysistechnologyoftheabaloneshellshasbeenawardedbythestateIntellectualPropertyOffice.Theproductfunctionswell,topeopleatvariousages,asbeingusedtoprotectliver,toimproveeyesightandtoalleviatesucheyediseasesasinflammation,inferioreyesight,cataractandshortsightedness。联系单位:厦门集美大学联系人:邱澄宇电话:0592-6188973传真:0592—618126318、大肠菌群和大肠杆菌检测新产品开发DevelopmentofnewproductionstodetectcoliformandEscherichiacoli大肠菌群和大肠杆菌是食品、药品、环境检测的重要卫生学指标.本项目于2002年获得国家863计划立项,经过2年研究,开发出能准确检测大肠杆菌和大肠菌群的培养基,快速、定量检测技术以及简便、实用的检测试纸产品。已经申请两项国家发明专利,一篇研究论文获中国海洋学会2003年学术年会优秀论文奖。这些成果适用于食品、药品、餐具以及环境样品中的大肠杆菌和大肠菌群检测,经过定型、转化后能够形成具有市场竞争优势的产品。ThehygienicindexesofColiformandEscherichiacoliareindispensableintheroutinedetectionoffood,drugandenvironmentalsamples.ThisprogramwassupportedbyChina’sHi-techResearchandDevelopmentProgram(863program)in2002.Afteratwo-yearresearch,productions(includingdifferentialculturemediaforcoliformandEscherichiacoli,rapidandquantitativedetectiontechnology,convenientandpracticalitydetectiontestpaper)hadbeenacquired.TwopatentshasbeenappliedandaresearchpaperwasawardedexcellencepaperattheannualacademicmeetingofChinaOceanographyAcademyin2003.TheseproductionsareapplicabletodetectcoliformandEscherichiacoliinfood、drug、dishwareandenvironmentalsamples.Anditisadvisabletoputthemintomanufacturefortheiradvantagesinmarketcompetition。联系单位:厦门集美大学联系人:王志勇电话:(0592)6180464传真:(0592)618126319、大黄鱼全雌化育苗技术Breedinggeneticallyimprovedallfemalefriesoflargeyellowcroaker大黄鱼雌鱼生长速度远快于雄鱼,本项目综合应用了选择育种、杂交育种、染色体组操作、分子标记辅助选择以及人工转性技术,将遗传改良和性别控制有机地结合,培育遗传性雌化优质良种大黄鱼苗.由本技术培育的大黄鱼苗雌性率达96.7~100%,养殖成活率显著提高,产量提高30%以上.Thegrowthspeedoffemalelargeyellowcroakerismuchhigherthanthatofthemaleones.Inourstudy,thetechniquesincludingselectionbreeding、crossbreeding、chromosomalmanipulation、molecularmarkerassistedselectionandartificialsexreversal,whichintegratesgeneticimprovementandsexdetermination,wereusedtobreedgeneticallyimprovedfemalefriesoflargeyellowcroaker。Theresultsshowedthatthefemalefriesratiois96.7%~100%andthesurvivalratioimprovedobviouslyandtheproductionincreased30%.联系单位:厦门集美大学联系人:王志勇电话:0592-6183816传真:0592-618147620、谷氨酰胺转胺酶的发酵生产及应用Theapplicationandfermentionoftransglutaminse(TGase)谷氨酰胺转胺酶(EC2.3.2。13,TGase)是一种可以催化转酰基反应,从而导致蛋白质分子内和分子间或蛋白质分子与氨基酸分子间发生共价交联的酶.因此,目前谷氨酰胺转胺酶已在水产品、肉制品、乳制品、植物蛋白制品等的加工及固定化酶、可食性包装品等方面找到了广泛而重要的用途。如果建立一个拥有3个30吨级发酵罐的中型发酵工厂,采用本项目技术进行TGase的发酵法生产,其经济效益测算(详略)结果如下:年产酶量(粗酶)1801116万酶活单位;年产值5400万元;年生产、出厂销售等总成本874。4万元;年收益4525.6万元。合作方式:寻求水产品或肉类食品添加剂生产或营销企业,合作开发,进一步提高TGase的产量,研制酶活稳定性高、适用于水产品和肉类加工生产应用的TGase商品剂型,开拓TGase的应用与销售市场,促进TGase的产业化生产与广泛应用.Transglutaminse(EC2.3.2.13,TGase)catalyzestransacylation,whichresultsinthecovalentlinkagesofintroandinterproteinsaswellasthecovalentlinkagebetweenproteinandaminoacids。TGaseisusedextensivelyinprocessingofaquaticproducts,meatproducts,dairyproductsandplantproteinproducts.TGasecanalsobeusedinthedevelopingoffixedenzymesandediblepackageproducts.Basedonourmarketanalysis,weestimateifafermentationfactorywiththree30m3Formatofcooperation:Wearelookingforcompaniesthatareproducingortradingofaquaticandmeatadditives.Withcollaboration,ourgoalistoincreasetheproductionofTGaseandtogenerateTGasewithhigheractivityandthermalstabilitytomeetthecommercialrequirementofTGaseinaquaticandmeatprocessing.Alsowithcollaboration,weareabletopromoteandopenupthemarketofapplicationandtradingofTGase。联系单位:厦门集美大学联系人:蔡慧农电话:0592——6180464传真:0592——618126321、海蛎壳综合利用技术IntegratedUtilizationofOysterShell福建省牡蛎壳拥有量全国第一,而牡蛎壳的开发因高能耗、高污染“瓶颈"难题而处于停滞状态。本项目在研究牡蛎钙的生物活性、毒理试验基础上,研究牡蛎壳在较温和条件下经无害处理,提制生物钙和无机盐的资源化利用技术。该技术具有低能耗,污染小、产率高的特点.TheoutputofOysterShellinFujianProvinceishighestinwholeChina,whereastheutilizationofOysterShellstagnatesbecausesuch“bottleneck”ashighenergyconsumptionandseriouspollution。BasedontheresultofresearchofbioactivityandtoxicologicaltestofOystercalcium,thisprojectaimstoresearchthetechnologyofextractionofOystercalciumandmineralsaltsfromOystershelltreatedbyhazard-eliminatingprocessunderamildconditioninordertoutilizeOystershellasaresource。Thistechnologyischaracterizedbylowenergyconsumption,slightpollutionandhighyieldrate。联系单位:厦门集美大学联系人:马建华电话:0592—-6180464传真:、0592—-618126322、海水养殖鱼类弧菌病快速检测及防治研究InstantDetectionandControlofVibriosisofCulturedMarineFish本技术主要应用于水产养殖中水产动物疾病的诊断及防治。用免疫学方法,利用抗原抗体特异性结合的原理,建立快速、灵敏、准确的检测病原的方法——酶联免疫吸附法(ELISA)和荧光抗体法(FAT)。可在疾病发生之前尽快地采取相应措施进行预防,避免疾病发生、降低发病率、减少死亡、减少用药、降低生产成本,减少经济损失。同时根据生物间的拮抗性及机体的获得性免疫原理,探索弧菌病的防治方法——生物防治方法和疫苗免疫预防方法,为建立有效的水产养殖动物防病方法提供新思路、新方法、新途径,为我国水产养殖业的健康、可持续发展奠定良好的基础。Thetechniqueisapplicabletothediagnoseandpreventionofaquaticanimaldisease。EnzymeLinkedImmunosorbentAssay(ELISA)andFluorescentAntibodyTechnique(FAT)thatdetectingpathogenrapidly,sensitively,preciselyareestablishedinaccordancewiththetheoryofantibodyspecificcombinationwithantigen。Beforethediseasesoccurthemeasuresaretakenforthesakeofpreventingdiseases,minimizingtheincidencesofdiseaseandmortality,reducingcostandeconomiclose。Meanwhile,biologicalcontrolandimmunepreventionareexploredonthebasisofbiologicantagonismandobtainedimmunityrespectively.Itispropitioustoestablishnovelandeffectivemethodsthatpreventculturedanimaldiseasesandsettlethegoodfoundationforaquaculturedevelopment.联系单位:厦门集美大学联系人:马建华电话:0592--6180464传真:0592—-618126323、海洋蛭弧菌微生态制剂的研制及应用技术ThedevelopmentandapplicationtechnologyofmarineBdellovibriomicroeclogicalpreparation水产养殖易受各种病原微生物的侵害,而各种抗生素及其他化学药物的使用势必造成二次污染。海洋蛭弧菌微生态制剂是利用该菌对水产动物致病性宿主菌天然的捕食及净化特性来进行水产养殖动物病害防治,其有效使用可以避免抗生素及其他化学药物的残留给人类带来的危害.本项目以水产动物体分离到的病原弧菌为宿主,从海水中分离出一株高活性菌株,利用该菌发酵液活菌制成纯天然的生物制剂。该制剂产品可应用于水产养殖细菌性疾病防治及促进生长,其技术含量高,实际应用效果显著,具有针对性且安全可靠,其技术达到国际先进水平。该微生态制剂的活菌浓度达到108pfu/ml以上,不含杂菌,其急性安全性实验结果显示无毒,在实验室条件下对细菌的杀灭率达到98.85%,在对虾育苗试验中使对虾育苗成活率平均提高了33.6%。Aquacultureiseasysubjectedtotheencroachmentofvariouspathogenicmicroorganisms。Howevertheuseofantibioticsandchemicalmedicamentdefinitelybringsaboutthesecondarypollution.ThemarineBdellovibriomicroecologicalpreparationcouldbeusedforthediseasepreventionandcureofaquacultureanimalbasedonitscharacteristicofthenationalpredationandpruicationtopathopoiesiahostbacteriumofaquacultureanimal.Theharzedcausedbytheuseofantibioticsandchemicalmedicamentcouldbegreatlyavoidedifthemicroecologicalprepparationhadbeenusedeffectively。ThisresearchprojectdevotestothedevolopmentofpurenaturalbiologicalagentwiththefermentationbrothofhighlyeffectivemarineBdellovibioseparatedfromtheseawaterwiththepathogenicvibrioasthehostbacteriumseparatedfromaquaticanimal。Thepreparationcouldbeusedforthepreventionandcureofbacterialdiseaseofaquacultureandpromotingthegrowthofaquacultureanimals。Theproductishigh-teched,effectiveinthepracticaluse,pertinency,safe,reliableandthetechnologyreachesadvancedinternationallevel.Theconcentrationoflivelybacteriuminthepreparationreachesabove108pfu/ml,withoutmiscellaneousbacterium.Theresultofacutesecurityexperimentdemonstratesthepreparationisinnocuous,anditskillratiotothebacteriumachieves98。85%undertheexperimentalcondition。Intheexperimentofshrimpartificialbreeding,itenhancedthesurvivalratioofshrimppost—larvaeby33。6%.联系单位:厦门集美大学联系人:苏国成电话:0592-—6180464传真:0592--618126324、九孔鲍的选育及复壮技术hebreedingandrejuvenationtechniquesofHaliotisdiversicolorsupertextaT通过挑选经济性状好的台湾九孔鲍和人工选育的九孔鲍亲体进行繁殖,避免近亲繁殖,选优除劣,达到正本清源的目的;采用正确的繁殖技术,获得优良的受精卵和幼虫,通过对幼虫进一步选优和培育,使附板后的幼鲍成活率及抗病力得到明显提高;为九孔鲍的苗种和亲本培育创造良好的养殖生态条件,减少和消除了不利的养殖环境和操作技术,达到品种复壮的目的;阐述了野生鲍、复壮鲍、退化鲍三个不同类群的免疫机能具有显著的差异,复壮后的九孔鲍,其免疫机能基本达到了野生鲍的水平;人工育苗和幼鲍的成活率提高了28.9%-31.2%;成鲍的养殖成活率高达85%—90%以上。Thoseparentabalonesofwildorartificialselectionwithgoodeconomiccharactersmustbechoseninordertoavoidclosebreeding..Correctbreedingtechniquesmustbeadoptedtoobtainexcellentgermcellsandlarvae。Thesurvivalrateanddiseaseresistanceofjuvenileabalonecanberemarkablyincreasedoncethefurtherselectingandculturingarecarriedout.Therejuvenationisrealizedonceagoodbreedingconditionfortheyoungandparentabalonesiscreated,theadverseculturingconditionandtechnicalmaloperationarereducedoreliminated。Thesurvivalrateofthebreedingandyoungabalonesincreaseabout28。9%-31.2%,thesurvivalrateofthegrownabalonesishighupto85%-90%.联系单位:厦门集美大学联系人:苏国成电话:0592——6180464传真:0592-—618126325、九孔鲍工厂化养殖的疾病研究ADiseaseStudyonIntensiveCultivationofAbaloneHaliotisdiversicoloraquatilis本项目针对九孔鲍工厂化养殖中发生于冬末春初的流行性病毒性疾病,采用环境生态保护、科学管理和药物防治等综合性治理方法,达到减少发病率,九孔鲍越冬成活率达80%以上。ThisprogramisdirectedatthepopularpathogenwhichnormallyoccursinlatewinterandearlyspringwhiletheabaloneHaliotisdiverscoloraquatilisisinintensivecultivation。Theincidentsofdiseasedecreaseandthesurvivalrateofthewinterabalonereachesover80%oncethecomprehensivewaysofecologyprotection,thescientificmanagement,andthemedicalpreventionandtreatmentarecarriedout.联系单位:厦门集美大学联系人:苏国成电话:0592--6180464传真:0592--618126326、九孔鲍健康种苗培育技术BreedingSkillofHealthyAbaloneHaliotisdiversicoloraquatilisSeeding本项目针对九孔鲍全人工育苗中发生大量死亡现象—“脱板症”,采用环境生态保护、饵料营养、药物和物理处理等防治技术,减少死亡率,鲍苗平均培育密度为1000粒/M2以上。Thisprogramisdirectedat“sheddingdisease”,aphenomenonofmassivedeathintheartificialbreedingoftheabaloneHaliotisdiversicoloraquatilisseeds.Thedeathrateistobereducedbyadoptingsuchpreventionandtreatmentskillsasprotectingtheenvironmentalecology,addingnutritiontothebait,providingmedicalandphysicaltreatment,etc。Theaveragebreedingdensityoftheabalonehaliotisdiversicoloraquatilisseedsisover1000persq.m。联系单位:厦门集美大学联系人:苏国成电话:0592--6180464传真:0592—-618126327、空肠弯曲菌的磁捕获—荧光PCR检测新技术NewTechnologyofMagneticCapture-FluorescentPCRDetectionofCampylobacterjejuni应用抗血清和磁性微珠自行制备弯曲菌免疫磁珠,利用弯曲菌免疫磁珠直接捕获检样中目的菌,不需要增菌培养;设计用于同时检测空肠弯曲菌鞭毛蛋白A(flaA)基因和马尿酸酶(hipO)基因的引物与荧光标记探针,通过荧光PCR检测flaA基因和/或hipO基因,建立空肠弯曲菌的磁捕获—荧光PCR检测新技术;该技术方法简便易行,可在一个工作日内完成,特异性好,敏感度高,抗干扰性强,可以鉴别区分空肠弯曲菌与其它弯曲菌,检测低限达到10cfu/mL,解决了活的非可培养状态或受损伤的空肠弯曲菌检测难题;该技术方法申请了发明专利(2.4),适用于检验检疫、疾病预防和农产品安全检验等领域的快速检测。InordertodeveloparapidmethodwhichcancheckCampylobacterjejuniinanimalandpoultryfoodsnicely,animmunomagneticcapture-fluorescentPCR(IMC-FPCR)methodwasestablished.ThemethodinvolvesisolationofthetargetpathogenbyimmunocapturepriortothefluorescentPCRstep,thereforetheimmunomagneticbeadsforCampylobacterweredeveloped,andtwogroupsofprimer/probe,whichtargetedforthespeciesspecialsequenceofflaAgeneandhipOgeneforCampylobacterjejuniweredesigned。Theimmunomagneticcapture—fluorescentPCRassayamplificationofthehipOgeneandflaAgenefordetectionofCampylobacterjejuniwasfirstlyreportedinthisstudy。ResultindicatedthatIMC-FPCRmethodpermitsdirectdetectionofthepathogenwithoutanenrichmentstepandcanbeperformedinapproximately24h.TheassayresultsarepositiveforalloftheisolatesofCampylobacterjejuni(3isolates,includingtypestrainATCC33560andATCC8341)withadetectionlimitofapproximately10cfu/mL,arenegativeforCampylobactercoliandseveralotherbacteria.IMC-FPCRassayprovidenotonlyarapid,sensitivemethodforquantitativedetectionofCampylobacterjejuni,butalsoanimportantmethodfordetectingofCampylobacterjejuniofviablebutnon-culturerable(VBNC)state.Themethodhasbeenappliedforapatent(2.4),anditissuitableforthefieldofinspectionandquarantine,diseasecontrolandprevention,agriculturalproductsqualityandsecurity.联系单位:厦门集美大学联系人:刘光明电话:(0592)6180464传真:(0592)618126328、龙虾养殖技术TheaquiculturaltechnologyofspinylobsterPanalirusstimpsoni龙虾(thespinylobster)是大型名贵虾类,市场需求量巨大,龙虾商品价格历来居高不下,人工养殖龙虾效益可观,前景看好.本研究成果达到国内领先水平。在陆上水池高密度养殖龙虾,种苗放养密度可达10尾/m2以上,养殖成活率可达90%,每虾年增重量高于300g,生产效能高于其它养殖方式。Thegiantspinylobster,Panalirusstimpsoni,isararemarineprawnspecies。Thereisavastforegroundindevelopinganaquiculturalprojectofrearingspinylobsterowingtothegreatdemandinfoodmarketaswellasitsperennialhighprices.Withaseriesofdoabletechniques,thelobstersistoberearedinsomeseaboardconcretepondsandthenumberreaches10persquaremeter,theaverageanimalsurvivalrateishighupto90%andthebodyweighsover300gafterinoneyearrearing。Allfactsbearingouttherearingefficiencyappliedtoaquiculturalpatternsurpassestheotherways.联系单位:厦门集美大学联系人:刘光明电话:0592--6180464传真:0592——618126329、坛紫菜新品种养殖技术CulturetechniqueofnewvarietyofPorphyrahaitanensis本项目利用人工诱变和体细胞单克隆技术等高新技术和传统的选择育种相结合的方法,分离出生长快、优质,高产,抗高温等的优良品种,在生产上大面积推广应用,并获得了分离坛紫菜稳定色素突变体的技术和建立坛紫菜的快速良种选育实用技术;新品种亩产提高26%-30%,3种主要色素(藻红蛋白、藻蓝蛋白、叶绿素a)提高10%以上,抗病烂能力较强。ThisprogramistoexplorethehightechandtraditionalbreedingofanewvarietyofPorphyrahaitanensis,inhopeofitsfastgrowth,highqualityandenduranceofhightemperature.Thisvarietyhasbeengrowingvariousareas,anditshowstha

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