Hispolon-DataSheet-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEHispolonCat.No.:HY-150521CASNo.:173933-40-9分⼦式:C₁₂H₁₂O₄分⼦量:220.22作⽤靶点:Antibiotic作⽤通路:Anti-infection储存⽅式:4°C,protectfromlight*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light)溶解性数据体外实验DMSO:125mg/mL(567.61mM;ultrasonicandwarmingandheatto70°C)MassSolvent1mg5mg10mgConcentration制备储备液1mM4.5409mL22.7046mL45.4091mL5mM0.9082mL4.5409mL9.0818mL10mM0.4541mL2.2705mL4.5409mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(protectfromlight)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。BIOLOGICALACTIVITY⽣物活性Hispolon⼀种多酚，可从桑(Phellinuslinteus)中分离得到。Hispolon具有抗癌、抗糖尿病、抗氧化、抗病毒、肝保护活性、抗糖尿病和抗炎活性。体外研究Hispolon(25and50μM,24-72h)inhibitscellviabilityofU87MGcells[2].Hispolon(25and50μM,24,48h)inducesG2/McellcyclearrestandapoptosisinU87MGcells[2].Hispolon(25and50μM,2-8h)decreasestheexpressionofG1–Stransition-relatedproteincyclinD4but1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEincreasestheexpressionofCDKinhibitorp21[2].Hispolon(25and50μM,24h)inhibitsthemigrationofU87MGcells[2].CellViabilityAssay[2]CellLine:U87MGcellsConcentration:25and50μMIncubationTime:24,48,72hResult:Inhibitedcellviabilityinadoseandtimedependentway.WesternBlotAnalysis[2]CellLine:U87MGcellsConcentration:25and50μMIncubationTime:2,4,8hResult:DecreasedcyclinD4level,andincreasedp21level.体内研究Hispolon(2.5-10mg/kg,i.p.)attenuatesLPS-inducedacutelunginjuryinmice[3].Hispolon(5and10mg/kg,s.c.)reducestumorgrowthinDBTRGxenograftmice[4].AnimalModel:LPS-inducedacutelunginjurymice[3]Dosage:2.5,5and10mg/kgAdministration:Intraperitonealinjection(i.p.)Result:AlleviatedthepathologicaleffectsintheLPS-challengedmouse.ReducedtheW/DratiointhelungandMPOactivity.Decreasedpro-Inflammatorycytokineproduction.AnimalModel:DBTRGxenograftmice[4]Dosage:5and10mg/kgAdministration:Subcutaneousinjection(s.c.)Result:Reducedtumorvolume(RTV).InhibitedGBMcellproliferationinvivouponHEandki-67staining.REFERENCES2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE[1].SarfrazA,etal.Hispolon:Anaturalpolyphenolandemergingcancerkillerbymultiplecellularsignalingpathways.EnvironRes.2020Nov;190:110017.[2].ArcellaA,etal.Effectsofhispolononglioblastomacellgrowth.EnvironToxicol.2017Sep;32(9):2113-2123.[3].HuangCY,etal.AttenuationofLipopolysaccharide-InducedAcuteLungInjurybyHispoloninMice,ThroughRegulatingtheTLR4/PI3K/Akt/mTORandKeap1/Nrf2/HO-1Pathways,andSuppressingOxidativeStress-MediatedERStress-InducedApoptosisandAutophagy.Nutrients.2020Jun10;12(6):1742.[4].LiaoKF,etal.HispolonInducesApoptosis,SuppressesMigrationandInvasionofGlioblastomaCellsandInhibitsGBMXenograftTumorGrowthInVivo.Molecules.2021Jul26;26(15):4497.McePdfHeightCaution:Producthasnotbeenfullyvalidated