Danshensu-Dan-shen-suan-A-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEDanshensuCat.No.:HY-N1913CASNo.:76822-21-4Synonyms:DanshensuanA;SalvianicacidA分⼦式:C₉H₁₀O₅分⼦量:198.17作⽤靶点:Keap1-Nrf2;Autophagy;Apoptosis作⽤通路:NF-κB;Autophagy;Apoptosis储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验H2O:5mg/mL(25.23mM;Needultrasonic)DMSO:1mg/mL(5.05mM;ultrasonicandwarmingandheatto80°C)MassSolvent1mg5mg10mgConcentration制备储备液1mM5.0462mL25.2309mL50.4617mL5mM1.0092mL5.0462mL10.0923mL10mM0.5046mL2.5231mL5.0462mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE1.请依序添加每种溶剂：PBSSolubility:10mg/mL(50.46mM);Clearsolution;Needultrasonicandheatto60°CBIOLOGICALACTIVITY⽣物活性Danshensu丹参的有效成分，能够激活Nrf2信号通路，保护⼼⾎管。体外研究Danshensu(DSS)significantlydecreasesthelevelofthemarkerenzymes(creatinekinaseandlactatedehydrogenase)fromthecoronaryeffluentsandmyocardialinfarctionsize.ThiscouldmarkedlycontributetotherecoveryofcardiacfunctionafterI/Rinjury.DSSalsohasROSscavengingactivityandboostsendogenousantioxidantssuchasSOD,CAT,MDA,GSH-PXandHO-1activitiesbyactivatingnuclearfactorerythroid-2-relatedfactor2(Nrf2)signalingpathwaywhichismediatedbyAktandERK1/2inwesternblotanalysis[2].体内研究AcutetreatmentwithasingledoseofdanshensuinratswithnormaltHcydoesnotchangeplasmatHcy.Incontrast,danshensusignificantlylowerstHcyinratswithelevatedtHcy.TherelativelyhighercysteineandglutathionelevelsaftertreatmentwithdanshensuindicatesthatitstHcy-loweringeffectisviaincreasedactivityofthetrans-sulphurationpathway[1].PROTOCOLAnimalAllchemicalsaredissolvedinsaline,exceptfortolcaponewhichisdissolvedinsalinecontaining20%(v/v)Administration[1]PEG200.Duringexperiments,ratsarefastedovernightandrandomLyassignedintodifferentgroups.About200µLbloodistakenfromorbitalsinusalternativelyafterethyletheranaesthesia,andthentheeyeisquicklysterilizedwithalcoholandpressedwithcotton.Bloodsamplesareimmediatelycollectedintoapolypropylenetubecontainingheparin-Naandcentrifugedat5000gat5°Cfor3min.Preparedplasmasamplesarekeptat−20°Candanalysedwithin48h.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.REFERENCES[1].YGCao,etal.Beneficialeffectsofdanshensu,anactivecomponentofSalviamiltiorrhiza,onhomocysteinemetabolismviathetrans-sulphurationpathwayinrats.BrJPharmacol.2009Jun;157(3):482–490.[2].YuJ,etal.DanshensuprotectsisolatedheartagainstischemiareperfusioninjurythroughactivationofAkt/ERK1/2/Nrf2signaling.IntJClinExpMed.2015Sep15;8(9):14793-804.McePdfHeightCaution:Producthasnotbeenfullyvalid