iFSP1-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEiFSP1Cat.No.:HY-136057CASNo.:150651-39-1分⼦式:C₂₀H₁₃N₅分⼦量:323.35作⽤靶点:Ferroptosis作⽤通路:Apoptosis储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:20mg/mL(61.85mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM3.0926mL15.4631mL30.9262mL5mM0.6185mL3.0926mL6.1852mL10mM0.3093mL1.5463mL3.0926mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:2mg/mL(6.19mM);Suspendedsolution;Needultrasonic1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEBIOLOGICALACTIVITY⽣物活性iFSP1FSP1(AIFM2)的选择性有效抑制剂，EC50值为103nM。iFSP1可选择性地诱导过表达FSP1的GPX4敲除细胞发⽣铁死亡(ferroptosis)。iFSP1可增强多种⼈癌细胞系对铁死亡诱导剂的敏感度，如:(1S,3R)-RSL3(HY-100218A)。IC50&TargetEC50:103nM(FSP1)[1]体外研究iFSP1(0.001-1μM;24hours)inhibitstheGpx4-knockoutcellgrowthasadose-dependentmanner,butdoesnotinhibitthewildtypecellgrowth.TreatmentwiththeferroptosisinhibitorLip-1protectsGPX4-knockoutcellsfromiFSP1-inducedferroptosis[1].iFSP1(0.001-1μM;24hours)islessefficientthangeneticdeletionofFSP1,whereasiFSP1treatmentintheFSP1-knockoutbackgroundhadnoadditiveeffecttoRSL3-inducedferroptosis[1].iFSP1(3μM;24hours)treatmentresultsinanobvioustoxicityofRSL3inapanelofgeneticallyengineered(FSP1-knockout)humancancercelllines[1].AIFM2:theflavoproteinapoptosis-inducingfactormitochondria-associated2isapreviouslyunrecognizedanti-ferroptoticgene.AIFM2,whichisrenamedferroptosissuppressorprotein1(FSP1)[1]CellProliferationAssay[1]CellLine:Wild-typeandGpx4-knockoutPfa1orHT1080cellsoverexpressingFSP1–HAConcentration:0.001-1μMIncubationTime:24hoursResult:Wastoxictocellsthatdependsolely(noGPX4expressiondetectable)onFSP1function.CellViabilityAssay[1]CellLine:NCl-H1437,NCl-H1437FSP1KO,U-373,U-373FSP1KO,MDA-MB-436,MDA-MB-436FSP1KO,SW620,SW620FSP1KO,MDA-MB-435S,MDA-MB-435SFSP1KO,A549andA549FSP1KOConcentration:3μMIncubationTime:24hoursResult:Sensitizedavarietyofhumancancercelllinestotheferroptosisinducer(1S,3R)-RSL3.户使⽤本产品发表的科研⽂献•CellDiscov.2022May3;8(1):40.•Small.2021Aug;17(32):e2101368.•CellDeathDis.2022May18;13(5):468.•CellDeathDis.2022Jan21;13(1):73.2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemESeemorecustomervalidationsonwww.MedChemEREFERENCES[1].DollS,etal.FSP1isaglutathione-independentferroptosissuppressor.Nature.2019Nov;575(7784):693-698.McePdfHeightCaution:Producthasnotbeenfullyvalidatedfor