SBI-115-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemESBI-115Cat.No.:HY-111534CASNo.:882366-16-7分⼦式:C₁₄H₁₃ClN₂O₄S分⼦量:340.78作⽤靶点:Gprotein-coupledBileAcidReceptor1作⽤通路:GPCR/GProtein储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:150mg/mL(440.17mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM2.9344mL14.6722mL29.3444mL5mM0.5869mL2.9344mL5.8689mL10mM0.2934mL1.4672mL2.9344mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(7.34mM);Clearsolution1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE2.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(7.34mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性SBI-115⼀种TGR5(GPCR19)拮抗剂。SBI-115通过抑制TGR5降低多囊性肝病的肝囊肿形成[1]。体外研究SBI-115(100-200µM,24hours)inhibitsproliferationtriggeredbypre-treatmentofcysticcholangiocyteswithTaurolithocholicacid(TLCA)inshRNA-transfectedADPKDcholangiocytes[1].CellProliferationAssay[1]CellLine:shRNA-transfectedADPKDcholangiocytesConcentration:100,200µMIncubationTime:24hoursResult:Inhibitedproliferation(by32-48%)triggeredbypre-treatmentofcysticcholangiocyteswithTLCA.户使⽤本产品发表的科研⽂献•NatCommun.2022Oct14;13(1):6081.•ActaPharmSinB.21July2021.•PharmacolRes.2022Sep21;106459.•iScience.2021,102508.•AquacultRep.February2022,100997.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].MasyukTV,etal.TGR5contributestohepaticcystogenesisinrodentswithpolycysticliverdiseasesthroughcyclicadenosinemonophosphate/Gαssignaling.Hepatology.2017Oct;66(4):1197-1218.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel