FM4-64-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEFM4-64Cat.No.:HY-103466CASNo.:162112-35-8分⼦式:C₃₀H₄₅Br₂N₃分⼦量:607.51作⽤靶点:Others作⽤通路:Others储存⽅式:-20°C,sealedstorage,awayfrommoisture*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoisture)溶解性数据体外实验DMSO:50mg/mL(82.30mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM1.6461mL8.2303mL16.4606mL5mM0.3292mL1.6461mL3.2921mL10mM0.1646mL0.8230mL1.6461mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoisture)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。BIOLOGICALACTIVITY⽣物活性FM4-64⼀种膜选择性红⾊荧光染料，属于苯⼄烯吡啶染料FM家族。FM4-64被⼴泛⽤于研究活体动物的内吞和外吞、囊泡运输和细胞器组织。体外研究FM4-64readilytranslocatestothenuclearenvelope(NE)atphysiologicaltemperaturewhereitexhibitsenhancedfluorescencewhenexcitedat620-650nmincontrastto480-520nmexcitationintheendocyticpathwayandintheendoplasmicreticulum(ER).Invitrodataindicatedthatthedyerevealsanenrichmentof1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEnegativelychargedlipids,presumablyduetolocalphospholipidsynthesis.Dual-excitationimagingofFM4-64inrelationtolamina-associatedpolypeptide-1-greenfluorescentproteinduringmitosissuggestedthatthedisassemblyofNEpreservesmicroscalelipidcomplexesintheER[1].Forstudiesonplantandfungalcells,FM4-64isusuallypreferredtoFM1-43duetoitssuperiorbrightness,greatercontrast,higherphotostability,anditsred-shiftedemissionfacilitatingconcomitantusewiththegreenfluorescentprotein(GFP).[3]REFERENCES[1].ZalT,etal.SpectralshiftoffluorescentdyeFM4-64revealsdistinctmicroenvironmentofnuclearenvelopeinlivingcells.Traffic.2006;7(12):1607-1613.[2].StenovecM,etal.DistinctlabellingoffusioneventsinratlactotrophsbyFM1-43andFM4-64isassociatedwithconformationaldifferences.ActaPhysiol(Oxf).2007;191(1):35-42.[3].BolteS,etal,Satiat-JeunemaitreB.FM-dyesasexperimentalprobesfordissectingvesicletraffickinginlivingplantcells.JMicrosc.2004;214(Pt2):159-173.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuse