D-AP5-D-APV-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemED-AP5Cat.No.:HY-100714ACASNo.:79055-68-8Synonyms:D-APV;D-2-Amino-5-phosphonovalericacid分⼦式:C₅H₁₂NO₅P分⼦量:197.13作⽤靶点:iGluR作⽤通路:MembraneTransporter/IonChannel;NeuronalSignaling储存⽅式:Powder-20°C3yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验H2O:27.78mg/mL(140.92mM;Needultrasonic)DMSO:<1mg/mL(ultrasonic;warming;heatto60°C)(insolubleorslightlysoluble)Ethanol:<1mg/mL(ultrasonic)(insoluble)MassSolvent1mg5mg10mgConcentration制备储备液1mM5.0728mL25.3640mL50.7279mL5mM1.0146mL5.0728mL10.1456mL10mM0.5073mL2.5364mL5.0728mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE1.请依序添加每种溶剂：PBSSolubility:100mg/mL(507.28mM);Clearsolution;NeedultrasonicBIOLOGICALACTIVITY⽣物活性D-AP5(D-APV)⼀种选择性和竞争性NMDA受体拮抗剂，Kd为1.4μM。D-AP5抑制NMDA受体的⾕氨酸结合位点。IC50&TargetNMDA[1]体内研究D-AP5isaNMDAreceptorantagonist.ChronicintraventricularinfusionofD-AP5causesaparalleldose-dependentimpairmentofspatiallearningandlong-termpotentiation(LTP)invivo.IntracerebralconcentrationsofD-AP5failtoinduceanymeasurablesensorimotordisturbancewhenspatiallearningisprevented[2].D-AP5infusionisassociatedwithaprogressivereductioninswimspeedovertrials.D-AP5causessensorimotordisturbancesinthespatialtask,butthesegraduallyworsenedastheanimalsfailtolearn.RatstreatedwithD-AP5showadelay-dependentdeficitinspatialmemoryinthedelayedmatching-to-placeprotocolforthewatermaze[3].PROTOCOLAnimalMaleListerhoodedratsareusedinthisstudy.TheyarehousedindividuallyandhavefreeaccesstofoodAdministration[1]andwateratalltimesexceptfor12hrpriortosurgery.Animalsareimplantedwith30mMD-AP5(N=6)andtestedinthewatermaze[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.户使⽤本产品发表的科研⽂献•Theranostics.2021Mar4;11(10):4616-4636.•SensActuatorsBChem.2020,129190.•BiomedPharmacother.January2022,112446.•CellDeathDiscov.2020Sep17;6:87.•CNSNeurosciTher.2022Nov15.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].DavisS,etal.TheNMDAreceptorantagonistD-2-amino-5-phosphonopentanoate(D-AP5)impairsspatiallearningandLTPinvivoatintracerebralconcentrationscomparabletothosethatblockLTPinvitro.JNeurosci.1992Jan;12(1):21-34.[2].MorrisRG,etal.N-methyl-d-aspartatereceptors,learningandmemory:chronicintraventricularinfusionoftheNMDAreceptorantagonistd-AP5interactsdirectlywiththeneuralmechanismsofspatiallearning.EurJNeurosci.2013Mar;37(5):700-17.2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE[3].RHEvans,etal.Theeffectsofaseriesofomega-phosphonicalpha-carboxylicaminoacidsonelectricallyevokedandexcitantaminoacid-inducedresponsesinisolatedspinalcordpreparations.BrJPharmacol.1982Jan;75(1):65-75.McePdfHeightCaution:Producthasnotbeenf