捕获和释放活性细胞的方法

Captureandreleaselivecells汇报人：于倩茹LIVECELLSCirculatingTumorsCells(CTCs)TLymphocytesCirculatingTumorsCells(CTCs)Circulatingtumorcells(CTCs)arecancercellsthatbreakawayfromeitheraprimarytumororametastaticsiteandcirculateintheperipheralbloodasthecellularoriginofmetastasis.Notion:Why?DetectingandanalyzingCTCsProvideinsightfulinformationinassessingthediseasestatusCTCsprovideconvenientaccesstoalldiseasesites现代技术中捕获和释放CTCs的主要方法根据分离原理基于物理性质分离基于亲和识别分离基于亲和识别分离利用癌细胞与正常血细胞的差别密度变形性粘附性尺寸基于物理性质分离核酸适配体抗体

NanostructureEmbeddedMicrochipsforDetection,Isolation,and

CharacterizationofCirculatingTumorCells基于亲和识别-抗体-实例Workingmechsnism: Whenthetwofabricstrips

ofaVelcrofastenerarepressedtogether,tanglingbetweenthe

hairysurfacesontwostripsleadstoenhancedbinding.The

proof-of-conceptdemonstrationoftheNanoVelcrocell-affinity

assay

liesintheuseofaSiNS,whichallowsforVelcro-likeinteractions

betweentheSiNSandnanoscalecell-surface

components.Here,anti-EpCAMwasgraftedontotheSiNSas

thecaptureagent,conferringspecificitytosuchacell-affinity

assayforrecognizingCTCs.Anti-EpCAMisgraftedontoaSiNStoconferspecificity

forrecognizingCTCs.Theanti-EpCAMgraftedSiNS

wasfabricatedthroughthreecontinuoussteps:(i)

introductionofdenselypackedsiliconnanowiresontoasilicon

wafer(ii)silanetreatmentandcovalentconjugationofstreptavidinontotheSiNS(iii)graftingofbiotinylated

anti-EpCAMontothestreptavidin-coatedSiNS.(a)Ananti-EpCAM-coatedSiNSwasemployedtoachievesignificantlyenhancedcaptureofCTCsincontrastto(b)ananti-EpCAM-coatedflatsiliconsubstrate。TLymphocytes

Tlymphocyteshavefundamentalfunctionsinimmuneresponsestomanydiseases,suchaspathogenicinfections,malignancies,andautoimmunediseases.DetectionsoftargetedTlymphocytesprovideimportantinformationinclinicaldiagnosis

.

TocaptureandmodifyTlymphocytestomakethemgetmorepowerfulPhotodegradableHydrogelsforCapture,Detection,andReleaseofCD4T-cellsandCD8T-cells实例：AntibodyprintingonthephotogellayerCellcaptureExposuretoUVlightCellreleaseExperimentProcedurePhotodegradableHydrogelsWeusedPEGhydrogel

asacoatingforprintingantibodyarraysandcapturingT-cellsfromaperipheralbloodmononuclearcell(PBMC)suspensionFormationofspotswithadiameterofapproximately150μmCellCaptureTheunderlyin