MIR96-IN-1-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEMIR96-IN-1Cat.No.:HY-15843CASNo.:1311982-88-3分⼦式:C₃₃H₄₈N₈O₂分⼦量:588.79作⽤靶点:MicroRNA;Apoptosis作⽤通路:Epigenetics;Apoptosis储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:≥100mg/mL(169.84mM)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制备储备液1mM1.6984mL8.4920mL16.9840mL5mM0.3397mL1.6984mL3.3968mL10mM0.1698mL0.8492mL1.6984mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%saline1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemESolubility:≥2.5mg/mL(4.25mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(4.25mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性MIR96-IN-1靶向miR-96(miRNA-96,microRNA-96)发夹前体结构中的Drosha位点，抑制其⽣物⽣成，及抑制下游靶标，并触发乳腺癌细胞的凋亡(apoptosis)。MIR96-IN-1结合到RNA1，RNA2，RNA3，RNA4和RNA5的Kd分别为1.3，9.4，3.4，1.3和7.4μM[1]。体外研究MIR96-IN-1(Compound1)selectivelyinhibitsproductionofmaturemiR-96andtriggersapoptosisinbreastcancercellsatmicromolarconcentrations[1].MIR96-IN-1(Compound3)bindsaUUloopinmiR-96andinhibitsitsbiogenesis[2].REFERENCES[1].SaiPradeepVelagapudi,etal.DesignofasmallmoleculeagainstanoncogenicnoncodingRNA.ProcNatlAcadSciUSA.2016May24;113(21):5898-903.[2].ChristopherLHaga,etal.SmallMoleculeInhibitionofmiR-544BiogenesisDisruptsAdaptiveResponsestoHypoxiabyModulatingATM-mTORSignaling.ACSChemBiol.2015Oct16;10(10):2267-76.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-8