NCBI序列提交方法

NCBI序列提交方法NCBI提交序列有两大类方式，一个是批量提交一个是单个序列提交。下面说的就是单个序列提交方式，这是最新的提交方式，由于NCBI更新很快，也许过段时间又不一样了，主要是按钮位置可能会变。首先要注册一个自己的NCBI账号：点击此处注册然后登陆账号:在已登陆状态下点击Submit点击图中标记处选择GengBank：

点击GO：5impieDMAorR\Asequencer5impieDMAorR\AsequencerQuickStartSubmis&icYaikm胃where\ouwanttogoseieclitnow\NeedhelpngMigoutGenBanK选择：Banklt：HowtosubmitdatatoGenB^nkThemostirrportancsotirceernevi-dataforGanBank®iscarpetsubmissionsNomscletilists.GenBankdependsmItsMnmbutorstonefpiketheda^abasEasEMrprehEnEive.rurreniand日匚curadm己与passible.NiCBIpno^klBstimelyandac匚urariEprarBSsingandbi口bgicalreviewafnewentriesandupM.esto材期ingentmes,习同晤readytoassistaulhors卜力口harvenew由曰t-osubmit.Rec&iviniganAccessionNumberforyourManij^criptfMostfou™i&requireOi4Asndarninoacidsequencesihat5neerredin3rtic的s*suDmmedloapuMlcsequencereposrtory(□□BJJEMBUGenbank-lNi£DC)partedhepubUcalionproc电的Daca改ch口俘bch^enDDBJ,EMBL^ndG@nBarikoccursdai^1soilonly tosvbrnrtthesequence1qonedglatese.wtiich的日onei&ec@coiwenientwthQiitregardE「Miwrethesequence呵Mpubllsned.sequencedatasubmlleflinadYanceofpublicationcanbeKeptconlicfentlaiirrequested.GenBanK%■provideaccessionmumbersTdrsubmittedsequences,u^llywiMin42'forkingdays.TMesaccessionnumbersmesasanidenllflefItir^oursutimllledyourdata,andallowsIherernmunit^'IdretrieveShesequenceuponreadinglirepumalarticleTheacEEssianinumbersiiouldbeincludedinycuimanusrnpt.preifeTaw^inaTcoinoteoninenrstpageoTtneartcie.orasreqLiredbyindividual>3Lmalprocedures..SujjmissiorKtoGinBariikwerearese/efStafiljonstofsubmittingda:atoGenBank.V•BanHiit-aVWW-liaseOsubmissioTitoolwithwanfeIdguide(he5ut?mt55ioriprocessincbi'saand-atonesubfns5>iant.odwithvisardsmgunelhesubmissionprocess羚available呼ftp的「useonTorbiftc.F1BI2谢.accmnaind-llMprogram,口戊的国臣inecreationofsequeincerecordsTwsutwfeGiflnmGenBankusingmsnyerfinesamefunctioreasSequin,it晤usedpnirnaril>!torsubmissionMgEplet巳gsngmesandlargeu用/besofseq依n国国andisavailable四FTforuse51MACPCandUnixplatTormsSLibirniMsiDn]Portal,aunifiiedsyslemformultipiesubrrissionitypesCumefiiHyonly16SribosomalRNAfrombacleriai'archaeacanbesKimittedwithWeGenSankcomponentofflustoolThiswillbeexpandediinrhefmrtiu吧toincludeothertypesofGeraBamnsubmissions.GenomeandTranscriptorTpeAssem国lescanbesubmittedinroughihe^VGSandTSApoflais.respectrw,eh1i.优皿白Submi占初前Tei",gUN审期上抽囱出0砧「M且subml驹121rl白r兜quanc屿nndt悟匕口/白口由in『口『日白旭白血口『Lt电口1句讨修ba作

建议认真阅读填写要求等：点击SignintouseBankltOH!Timiy^l b.I7<£€alpTopics.旧l~gseaH-KEr而tV&l工击韬G^riBarikGubhi唇却OH!Timiy^l b.I7<£€alpTopics.旧l~gseaH-KEr而tV&l工击韬G^riBarikGubhi唇却bhOptlC9hfiirpm比r「叩tort・制/由。山&MEITyouha-a-esnyqueslians-ztcul11511gtheBantitsequencesubmE»anIimIpk-zseEatzclGerEankUsersenkiKaliniiliigfHhinlEntrjzanduseEankffandyourE-inkttI口才inItiesubletInenncKMiiOin3hipm10#w林+V 僧*Htwrtucym…日用jqmM用白中用归鹏1OnceaSAJbmmerreui&wrs.tou的Ba^lLl幅sutoritiersco他tirWrnBooii原潮电口and悖auLomellce^©的口图eeth初heeqi昭MHmg巾由&ucEMr料恪In例如）Eh2BsrckllEanbeusedfa知bmilbolliastngh-EequsKeandaxeicfsequtfices-inoneM±fTTi!i5lon3网料iBfcepis■例mequ刖Her8Miequenc^^旗般mawm弟帕用党网1Wsaupk>we4^快i4Barikllnlnws-asutmffierIdsJbopinIhemicclEarasubflilssionandtocc«itlnu=wthU^q£submEunnalaIderlire3BarckllnluwssubmrtlerxwguIhelabs：afthfleperthesubnx-siannpulimagesIdnnvignluandIdedtpmtauslyYEiiedpages.userswtatunreexstingNCBIFDAshcukiuseWie&am&=OAUsernameandPass*orai协通MMyMl：Ellnow.LJMbiiXW\i।的Kw.Pr白UseBamltlf:»youhaKasingbsequencEasimplenrsequencesjhirexample-IESrRNA,malKHEi'rflNA,amoEqtborCE4s«iBKaraamslbaknairrerentBegifeoceB♦youwuse日%电际总0占ucrft&shnkxm・lieteaiircannolaicnRirynursequencesenDlcoir^icalEd・youdonnlnt^uireadvar>：Edsequenceana^sislo>DtM・阳并和u联弧叫我所rnzi6S同^3equeiKe4丽&pfc*fliyffllckupcs.pfeaseIrvins轴所13mtohHuifubE%rcbidmnlh炉白^^石磔仙前小■r-iiiiirl-:i加加M：i：:K1Hlm内轲巾店口UHEEEnli点击NewSub,下面是你提交的记录，即使你提交过程中中断了，也可以Continue!以下是具体提交过程:一..填写基本信息ConteattFWerenceSequercirglKhnok?g>咐ucioMdrO«啊ni5nl尔由rnMcn SajnceMSubmi^ion#1885945ContactInformationFirstNorriel-hunhui |ustNamt|nuang |lenuzhovunMeiw 1InitituliohIscncfliarBid&fckki三国门日印国StreetAddrtss-enifeAvenueC叫CJ1LGIM41田-M |Zl宜串8MliCode.3BQOT|Cwntiy忙hmaFhatne侬—蜡111 15画邈避种中比001加融敝继顽Emdil|mchhm^1&2>.corrprcvKiean面归(亡mailac*drc55toetisure-Ihatntssa9csartrwewd.屈曜『迎仲Emaiil I.图中1是作者名字，可以添加作者名，First和LastName,填了就可以，其他可以不填。.图中2是填是否出版，有三个选项：未出版，正在出版，已经出版。需要注意的是，如果选则未出版，需要在Title中注明，否则无法提交。.可以添加作者，然后Continue即可。SequenceAuthor;FirstNameMiddlemmal[3}LastNameSUHIXRernav^AAA—|AA7J因AtldJmoresequenceauthors.ReferenceInformation#1Pleaseprouidettietitleandrelevantpublicationdetanlsj'volume,issueetc)ofapaperifi^PUBLI!CATIONSTATUSUnpublishedQIn-Press•'PubtoheeF—ReferenceTine iunpublishedREFERENCEAUTHORS®SameAsSequenceAuthorsOSpecif^NewAuthors皿|AiwtruRm「ence|qCemtinue

三.这是选择是用生么测序方法后的的序列.现在我们用的一次只能测一条序列的都是Sanger测序，下面的454等都代表仪器型号,是我们现在说的高通量测序，简单的书不同型号灵敏度不同。.这是选择你测序是一个反应还是几个反应，就是你这条序列是一个测序结果就出来了，还是几个测序结果拼在一起得到的结果，一代测序一个反应最可信的测序范围是50-750bp,根据实际选择，然后继续。assembledsequencer(consistingoftwoormores&quencereadsjSequencingTechnologyThismfairmationisrequiredifyouaresubmittingoverSODsequencesorrfyoursequentassembledsequencer(consistingoftwoormores&quencereadsjSequencingTechnologyThismfairmationisrequiredifyouaresubmittingoverSODsequencesorrfyoursequentWhatmethodswereusedtoobtainthesesequences^3454Htlicoi□llluminalonTorrent□PacificBio$cianc4«SOLiD□other|Continu日Arethesesequencers);：)unassembledsequence四.填写序列信息：.选择什么时候公布你的序列信息，你可以选怎立刻，也可以选择在以后的某一时间，如果你选择了时间，那么即使你已经上传序列，有了序列号，在时间未到的时候你也是找不到的。.选择你的序列是不是16SrRNA基因是细菌上编码rRNA相对应的DNA序列U,可能有一个专门的库，所以一般选择NO.选择你的序列类型，1.我选了cDNA,然后选择是什么类型：是线性，我们一般的都是线状的，还是环状一般想线粒体，叶绿体或者各种质粒都是现状的。

.然后填写你上传了多少个序列，黏贴序列，注意序列一定要是FASTA格式。你也可以选择上传文档，这个自己学。然后继续两次继续。SubmissionReleaseDateWtitnmayw。r鼻旧口5。yflur 事rscgrci?)9户)9户9分七户RalFfnnmaliniTi-Mrin-YVW,(i？namfilph-7nnd)16s「RNA£ubmi&sion&Ar&]h&sequencesinWissabmisgiDnONLY1SS/IbnsamalRNAdaoa'?0¥电中⑥MuSequences}andDefinitionLine⑸MoleculeType：CDNAtOnriRNAMoleculeType：CDNAtOnriRNATopology:LinearTopology:Linear"Ggnomicamp修悼n/s：PartialNuchotid@Sequent餐(芋)mndDefinitionLinesSequencesmustbeenteredintheFAST再TarmatwhetherycuGgnomicamp修悼n/s：PartialNuchotid@Sequent餐(芋)mndDefinitionLinesSequencesmustbeenteredintheFAST再Tarmatwhetherycuaie■iubmiHing日singlesequandwmultiple专m□Efinitionwhichareu«dtodescnSeeachsequence.sTiauldUeincludedmtheFASTAftnrnaLHowiinaniy力mcI0白沙壮自£«quen€tsd^youintendtos#ndinthis,submission?|129了PasteSequencefs)>OSPIP2-1~: JacaagctctcCgctcagctaagctctccaccattgcttgcaaagctgaGTCGTGTCACTCCTCCCGTTMGCTTCCCGCGGCGGCGAGAGCAAGCTAAGCTAGGTCGGGCATGGGGAAGGACGAGGTGATGGAGAGCGGCGGCGCCGCCGGCGAGTTCGCGGCCAAGGACTACACGGACCCGCC7五.填写你上传序列所属物种。然后即使继续。GenBankSubmissionsContactRefenerKeSequencingTechnologyNucleotideOrgani5inSubmission#1885945FillinmiissingOrganisminformationyoudidnotincludeuienameor：heorganismironwmcntnesequencewasisolated.PiiOrganiBmN3FT1G|Whe才CanlinueRntactICvpyirialit|Di5d^irn>「|PijvecY|具css■对bilit/^latiorqICcntarfccSliQiachnologryInibirnoticn,U£NaticHolLibraryofhJedicinc：MDRoccvlteFiteraechesda.MDUSft2QS&4六.选择你要你的序列式原始序列，就是你自己测序的序列，还是为已有序列做注释。然后继续。GenBankSubmissionscontactReferencesequencingTecrrKlogyNucleotideorganismsubmissioncategorySubmission#1885945SubmissionCategoryndicaiewhetheryoursequenceisanonginalsubmissionora:hirdpartyannoLationsutmlss<•Original「ThirdPartyAnnotationDirectlysequencedbysubmitter.<•Original「ThirdPartyAnnotationJenvedfromotherpFima[ysequencedata.untacl(:皿riulit口/claim。|Priveizv|EtcnsiGefiterfixEiiretinnolo即 .us"期LibrarynrfvedfcsoeIDORoaEHPik*3a±.fi£cla,USA2CSQ4七.1.2.3.序列信息七.1.2.3.序列信息填写你基因所属细胞器填写你对基因的注释，可以添加选项，根据自己实际情况添加。最下面可以添加你的PCR引物，然后继续，会让你检查，你检查后继续即可。uentsanKSubmissionsCont5clReteienceSequaiclngTechnology.1NucleotideGrgenisinSWnissionCatego^souretSubmission#18S5945SourceModifiersSourceOrgnmellei'LoDSticinIntDrmitionChooðe—nrect后o「icx.al町n.rfapplicableOrganellefLoeatiDnimilaOrganellefLoeatiDnimilaehandrionS-dufgbModifiersChoosesourcemodinerfs)anc3entervaii>e{s}herePleaserefertoS口urc,已Eochf旧r「或farde^cnptraniandfarmartsourcerngdififrva]uiARernovtB*omaterialsourcerngdififrva]uiARernovtB*omaterialwhaet阳danothermolii■匚emcdifieiAn*olhersourcerelaledinTocniation?|Md|ForexampieTaKonomiEClassifkationorLineageforunftndwni'unrecognizedargani5jmsYdushouldcrealtu&e'note1asIheheaderlabel.D。ii@ftenterIndi*uidualsourcemodifflersasp忸Intexthere.Chocrae1hemTrotIfyouwant比IncludePCRprimersimyoursubmlsiiion,theckhere.口k?rinMnriH八.信息补充.填加序列信息，可以上传文件，也可以选项填写，建议用选项。.选择你的信息，可以添加，然后继续Pleaseprovidlefeatureanncrt^tioosfcryQursubmissionbychoosingqh®ofthetwoQAddfeaturesbyuploadingnvecolumnTeatmretablefieD：*：,Addfeatures compllelflnginputr口「m号 JThismethodisbestsuitablefen:asinglefeatureor百fewteaturesappliedonasinglesequencethesamesinglefeatureorthesamefewfeaturssappliedtoallsequencesirFEdtu「e5cambeaddedacrossanentiresequenceorbyspecificintervalswit・oneormorequalifierscanbechosentoapplytoeachfeature,ifitisnotelecTo3ddafe^lure.selectfeature匚alEgciryandtearturelypewithinthatcatego^tti'：,codingRegK>n(CDS)fGeneJmRNA—Ilyoursequenceencodesapratein,@RNA(rRNA,tRNA,non-codongRNA,mi5C_RNA,etc)|m妇写5巳口口巳「Rhia 3 3二'Repeatregion(forsequencere-pe-ats.,mobileelements