DFHBI-1T-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEDFHBI-1TCat.No.:HY-110251CASNo.:1539318-36-9分⼦式:C₁₃H₉F₅N₂O₂分⼦量:320.21作⽤靶点:Others作⽤通路:Others储存⽅式:-20°C,protectfromlight*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light)溶解性数据体外实验DMSO:50mg/mL(156.15mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM3.1230mL15.6148mL31.2295mL5mM0.6246mL3.1230mL6.2459mL10mM0.3123mL1.5615mL3.1230mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(protectfromlight)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:5.5mg/mL(17.18mM);Suspendedsolution;Needultrasonic1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEBIOLOGICALACTIVITY⽣物活性DFHBI-1T⼀种可透过膜的RNA适配体激活的荧光探针(ex/em=472nm/507nm)。DFHBI-1T与RNA适配体(Spinach,Spinach2,iSpinach,Broccoli)结合并产⽣特定的荧光和较低的景荧光。DFHBI-1T可以⽤于活细胞中的RNA成像。体外研究DFHBI-1T(20μM;for10min)increasesfluorescenceinCOS7cellsexpressing(CGG)60-Spinach2overDFHBI(20μM)[1].Broccoli-DFHBI-1Thasex/em=472nm/507nmandSpinach2-DFHBI-1Thasex/em=482nm/505nm[2].户使⽤本产品发表的科研⽂献•BiosciRep.2021Sep1;BSR20211525.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].WenjiaoSong,etal.Plug-and-playfluorophoresextendthespectralpropertiesofSpinach.JAmChemSoc.2014Jan29;136(4):1198-201.[2].GrigorySFilonov,etal.Broccoli:rapidselectionofanRNAmimicofgreenfluorescentproteinbyfluorescence-basedselectionanddirectedevolution.JAmChemSoc.2014Nov19;136(46):16299-308.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820