Capsaicinoid-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemECapsaicinoidCat.No.:HY-10448ACASNo.:404-86-4分⼦式:C₁₈H₂₇NO₃分⼦量:305.41作⽤靶点:TRPChannel;Autophagy;Apoptosis作⽤通路:MembraneTransporter/IonChannel;NeuronalSignaling;Autophagy;Apoptosis储存⽅式:4°C,protectfromlight*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light)溶解性数据体外实验DMSO:100mg/mL(327.43mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM3.2743mL16.3714mL32.7429mL5mM0.6549mL3.2743mL6.5486mL10mM0.3274mL1.6371mL3.2743mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(protectfromlight)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(8.19mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemESolubility:≥2.5mg/mL(8.19mM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(8.19mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性Capsaicinoid辣椒素和⼆氢辣椒素的混合物，⼀种辣椒素受体(TRPV1)激动剂。体外研究CapsaicinisthemainCapsaicinoidinchilipeppers,followedbyDihydrocapsaicin.Thesetwocompoundsprovideabouttwicehotnesstothetasteandnervesastheminorcapsaicinoids[2].CellViabilityAssay[2]CellLine:Humanpharyngealsquamouscarcinomacells(FaDu)cellsConcentration:50µM,100µM,200µM,and300µMIncubationTime:24hours,48hoursand72hoursResult:Showedanaugmenteddecreaseincellgrowth.ApoptosisAnalysis[2]CellLine:FaDucellsConcentration:50µM,100µMand200µMIncubationTime:12hoursResult:Increasedtheactivityofcaspase3inatime-dependentmanner.WesternBlotAnalysis[2]CellLine:FaDucellsConcentration:200µMIncubationTime:24hoursResult:Theobservedactivationofcaspase3andPARP(p85)levels.户使⽤本产品发表的科研⽂献•Neuron.2020Nov25;108(4):707-721.e8.•Theranostics.2020Jun24;10(17):7906-7920.•CurrBiol.2021May14;S0960-9822(21)00601-1.•JInflammRes.2021Oct14;14:5291-5304.2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE•StemCellRevRep.2021Jun;17(3):999-1013.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].HoyounCho,etal.DevelopmentofadatabaseofcapsaicinoidcontentsinfoodscommonlyconsumedinKorea.FoodSciNutr.2020Jul16;8(8):4611-4624.[2].KrishnapuraSrinivasan.BiologicalActivitiesofRedPepper(Capsicumannuum)andItsPungentPrincipleCapsaicin:AReview.CritRevFoodSciNutr.2016Jul3;56(9):1488-500.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedical