过往文献细胞生物vamp721-k channelsben zhang2015plant cell

1、V721-K+Channels+ BenZhang+2015+PlantTheArabidopsisR-SNARE721 eractswithKAT1andKC1K+ChannelsModerateK+thePlasma拟南芥R-SNARE721与KAT1andKC1K+通道相互作用在质膜上调控K作者：BenZhangRuchaKarnikYizhouWangNiklasWallmerothV721-K+Channels+ BenZhang+2015+PlantTheArabidopsisR-SNARE721 eractswithKAT1andKC1K+ChannelsModerateK+th

2、ePlasma拟南芥R-SNARE721与KAT1andKC1K+通道相互作用在质膜上调控K作者：BenZhangRuchaKarnikYizhouWangNiklasWallmerothMichaelR. BlattandChristopherSNARE 蛋白功能摘要，SNARE SYP121 通过与 K+通道物理接触调控质膜 gating. SNAREsK+ channels相互作用，但gating质膜，作用并抑制 inward-rectifying K+ channels KAT1 and KC1 的活性。此相互作用在 yeast ubiquitin assay中很明显，并通过ratio

3、metric bimolecular fluorescence ion互补作用恢复对单残基Tyr-57突变敏感，Tyr-57the of 721. 此作用也可通过交换723 721723reticulum内质网膜。降低的通道活性及改变的voltagesensitivity，通过channelgates了很好的解释。这些actions 补充了SYP121, 721的同系partner，指引作者们提出，two proteins在channelcontrol小编小结：从SNARE 介绍，到SNARE SYP121 介绍，另一类SNAREs 介绍，到本文的序渐进，写的、做的都很 remarkable、

4、excellents提出723，“hand-off”，第一段，SNAREdrivesvesicle.第二段，R-like R-SNAREs，8个主要作用是质膜上的泌作用。写作好严谨，比如：Barringafewnotableexceptions，withfewexceptions第三段s，在植物中的生理活性，are linked directly with membrane vesicle traffic，or can understoodasadirectconsequenceof itsregulation第四段Qa-SNARESYP121介绍，trafficandtransportcon

5、trolbySYP121canbeuncoupled第五段721 and 722 assemble in SNARE core complexes with SYP121 and with closest homolog SYP122，提出问题，本文，作者们提出，V eract with the channels, affecting channel gating physiologicalvoltagerange，提出Tyr-57的关键性，以及721 and 722, but not suppressing the K+ current within 721 and小编小结：深入浅出的写作使

6、读者产生很大，想迫不及待的看下面的研究 tedwith -K+ eractionsinV+ SUS，K+channelsKAT1andKC1，SYP121，the72subgroup，plasma第一步筛选得到diploid grew well eraction-selective media when carrying the K+ Cub swith Nubsof 721,722,724,and然后，Theseresults indicatedconsistent anderactions of the K+channels, notably withplasmamembrane-ted7

7、21and722,SUS，K+channelsKAT1andKC1，SYP121，the72subgroup，plasma第一步筛选得到diploid grew well eraction-selective media when carrying the K+ Cub swith Nubsof 721,722,724,and然后，Theseresults indicatedconsistent anderactions of the K+channels, notably withplasmamembrane-ted721and722,bycontrast with t localizest

8、oendoplasmicTherefore, we focused on eraction with 721 and used 723 as a control to sandfunctionalityofthechannel-721与KAT1andKC1KChannels相互作2in1vectorsystem，YFP,RFP烟草瞬时表达Consistent withtheSUSassays,KAT1with721gaveastrongrBiFC作者们 t 721 eracts with the K+ channels, in vitro and in vivo, V723does721影响K

9、AT1KChannel V好高端的实验设计recorded K+ currents under voltage after heterologously expressing and 723withtheKAT1in XenopuslaevisThese results t 721 affects both the KAT1 litude and rinsic propertiesinastoichiometric提出Coexpressing 721 Suppresses KAT1 K+ Current and Alters Channel Gating in X. 标题四：Theof V72

10、1IsEssentialforK+ 721的关键The ofaseries -the(L),the (S),andthe标题三721AffectsKAT1K+Channel标题二721,butNot723, eractswith KAT1 andKC1K+Channelsin作者们发现 diploid yeast growth was recovered t the chimera incorporated ofTo validate the eraction in vivo, roduced chimeras incorporating the 721 V723 s, 721L723SMan

11、do the rBiFC2in1vectorTheresultsdemonstrate a highly significant rBiFC signal above the negative controls when was coexpressed with t includes the 721 , but not 作者们发现 diploid yeast growth was recovered t the chimera incorporated ofTo validate the eraction in vivo, roduced chimeras incorporating the

12、721 V723 s, 721L723SMando the rBiFC2in1vectorTheresultsdemonstrate a highly significant rBiFC signal above the negative controls when was coexpressed with t includes the 721 , but not thecomplementary723L721SMt includesthe723.The of 721 is a primary determinant of the eractionn and 标题五：Tyr-57of 721I

13、sEssentialforK+ Tyr-57of 721的关键isolatetheheofonly residues within the central LB segment of the 721 were essential for his segment (the central LB segment) there are four amino t differ n and To validate these findings in vivo, we again incorporated the corresponding single-site substitutions the2in

14、1vectorcarryingKAT1-cYFPfortransienttransformationoftobaccoysisbyWerepeatedtheseexperiments usingtheKC1asthebaitinSUSriguingquestionarisingfromthesedatarelatestotheimplicit relocalizationof themutant eastof723D57Y,andtheir distributionsattheplasmaThus, we t Tyr-57 is an essential and key determinant

15、 for R-SNARE binding with both theK+channelsandfficienttoengineerchannelheotherwisenon eracting标题六：K+Channel ActivityDependsonTyr-57ofHeterologousandcoinjectedeachoftheurnwithKAT1cRNAinX.laevis with 721 and 721Y57A reduced the current at any one voltage, displacing the steady e current-voltage curve

16、 to the right, but with 723 V721Y57Dwaswithout标题七721andK+ eractionInhibitstheK+heoverexpressed721,723,andhetheK+current wasstronglysuppressed when721wasexpressed, yieldingonlya smallalldisplacing the steady e current-voltage curve to the right, but with 723 V721Y57Dwaswithout标题七721andK+ eractionInhi

17、bitstheK+heoverexpressed721,723,andhetheK+current wasstronglysuppressed when721wasexpressed, yieldingonlya smallallbutthemost negativewhethertheeffectsontheK+currentmighttranslatetochanges inK+-dependentThus, the effects on the K+ channels of 721 and its Tyr-57 mutant appear to translate directly gr

18、owthwhenthechannelsarethedominant pathwayforK+小编小结：实验设计严密、完整，写作思路清晰简洁，先说实验设计的可靠及意义，再总结概括结果，并提出一个观点结论。研究的重心很集中正反反复验证，体内体外双向验证。实验的难度很大很有分量，文章的亮点能连成Qa-SNAREs；Asubset ofQa-SNAREs；Qa-SNARE SYP121；SYP121 binds directly withtwo rectifyingK+channels, KC1andMuch less is known of the physiology and functional

19、 t o the cognate partnersinplants,notablytheThe near-identical homologs 721 and 722 are known to assemble SNARE core withSYP121todrivevesicleattheArabidopsisplasmaWe t (1) 721 and 722, but not eract with the channels, and t 721 affects channel gating and the K+ current he physiological voltage and i

20、t suppresses seedling growth when channel-mediated K+ uptake predominates; (2) binding is ted with the of the , specifically with Tyr-57; and(3) of this residue alone are sufficient to engineer binding and K+ channel control in the non eracting 723. We teractionofthese s withthe K+ channels, tof SYP121, is complementary to their roles in vesicle traffic. We suggest, t 721 V722 binding with the channels may contribute to a hand-over in channel contr