FIN56 - Ferroptosis Inducer - 生命科学试剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEFIN56Cat. No.: HY-103087CAS No.: 1083162-61-1分式: CHNOS分量: 517.66作靶点: Ferroptosis作通路: Apoptosis储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 100 mg/mL (193.18 mM)H2O : 40% PEG300 5% Tween-

2、80 45% salineSolubility: 2.5 mg/mL (4.83 mM); Suspended solution; Need ultrasonic2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (4.83 mM); Suspended solution; Need ultrasonic1/2 Master of Small Molecules 您边的抑制剂师www.MedChemE3. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.5 mg

3、/mL (4.83 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 FIN56种特异性的铁死亡诱导剂。体外研究 FIN56 causes the loss of GPX4 activity in cell lysates. FIN56-induced cell death is suppressed by GFP-GPX4fusion protein overexpression. FIN56 triggers ferroptosis through a mechanism involving the regulation ofGPX4 protein ab

4、undance 1.PROTOCOLCell Assay 1 1000 cells/36 L are seeded in each well in 384-well plates. Lethal compounds are dissolved and a 2-fold,12-point dilution series are prepared in DMSO. Compound solutions are further diluted with media at 1:25and 4 L/well of the diluted solutions are added to cell cultu

5、res immediately after cells are seeded. Whenferroptosis inhibitors (100 M -tocopherol, 152 M deferoxamine, or 10 M U-0126) are co-treated withlethal inducers, they are supplemented to cell culture at the same time as lethal compounds are added, andthe cells are incubated for 24 hrs. When other cell

6、death modulating compounds (100 nM sodium selenite, 1M cerivastatin, 100 g/mL mevalonic acid) are co-treated, they are first supplemented to cell culture for 24hrs before lethal compounds are added to cell culture and further incubated for 24 hrs at 37C under 5%CO2. On the day of the viability measu

7、rement, 10 L/well of 50% Alamar Blue diluted in media is added andfurther incubated at 37C for 6 hrs. Fluorescence intensity (ex/em: 530/590) is measured with a Victor 3 platereader and the normalized viability is calculated by VL = (IL-I0)/(IV-I0), where VL, I0, IV, and IL are thenormalized viabili

8、ty, raw fluorescence intensities from the wells containing media, cells treated with a vehicle(negative control), and cells with the lethal compound (L), respectively. When the effect of a chemicalmodulator (M) on L is calculated, we instead used the equation: VL|M = (IM,L-I0)/(IM,V-I0), where VL|M,

9、 IM,Land IM,V are the normalized viability, and fluorescence intensity from cells treated with M and V, and fromcells with M and L. respectively. The viability is typically measured in biological triplicates unless otherwisespecified. A representative dose-response curve, the mean and standard error

10、 of normalized viability fromone replicate are plotted.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Shimada K, et al. Global survey of cell death mechanisms reveals metabolic regulation of ferroptosis. Nat Chem Biol. 2016Jul;12(7):497-503.McePdfHeightCaution: Product has not been fully vali