Pyridostatin-hydrochloride-RR-82-hydrochloride-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEPyridostatin hydrochlorideCat. No.: HY-15176ACAS No.: 1781882-65-2Synonyms: RR-82 hydrochloride分式: CHClNO分量: 778.94作靶点: G-quadruplex作通路: Cell Cycle/DNA Damage储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month

2、溶解性数据体外实验 H2O : 31 mg/mL (39.80 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 1.2838 mL 6.4190 mL 12.8380 mL5 mM 0.2568 mL 1.2838 mL 2.5676 mL10 mM 0.1284 mL 0.6419 mL 1.2838 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Pyridost

3、atin hydrochloride种 G-quadruplexe 稳定剂，Kd 值为 490 nM。IC50 & Target Kd: 490 nM (G-quadruplexe) 1体外研究Pyridostatin hydrochloride is a G-quadruplexe stabilizer, with a Kd of 490 nM 1. Pyridostatin (PDS) shows1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEneurotoxic activity against primary cortical neur

4、ons at 0.01-5 M, causes DNA double-strand breaks (DSBs)at 1 M, downregulates BRCA1 in neurons at 1, 2 or 5 M 2. Pyridostatin interacts with G-quadruplex motifsin SRC and alters mRNA levels of damaged genes 3.PROTOCOLCell Assay 3 Cells are plated at equal confluence and either untreated or treated wi

5、th 2 M Pyridostatin continually for 72h. Cells from individual plates are trypsinized and counted in a Coultercounter. Graphs represent total cellnumbers at each time interval and error bars represent S.E.M. Data represent three independentexperiments 3.MCE has not independently confirmed the accura

6、cy of these methods. They are for reference only.户使本产品发表的科研献 Nucleic Acids Res. 2019 Jun 5. pii: gkz484. J Mol Med (Berl). 2019 Jun 14.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Koirala D, et al. A single-molecule platform for investigation of interactions between G-quadru

7、plexes and small-molecule ligands. NatChem. 2011 Aug 28;3(10):782-7.2. Moruno-Manchon JF, et al. The G-quadruplex DNA stabilizing drug pyridostatin promotes DNA damage and downregulates transcriptionof Brca1 in neurons. Aging (Albany NY). 2017 Sep 12;9(9):1957-1970.3. Rodriguez R, et al. Small-molecule-induced DNA damage identifies alternative DNA structures in human genes. Nat Chem Biol. 2012Feb 5;8(3):301-10.McePdfHeightCaution: Product has not been fully validated for medical applications.For rese