RI-2 - RAD51 抑制剂 - 生命科学试剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemERI-2Cat. No.: HY-16904CAS No.: 1417162-36-7分式: CHClNO分量: 433.28作靶点: RAD51作通路: Cell Cycle/DNA Damage储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 15.5 mg/mL (35.77 mM; Need ultrasonic and

2、warming)Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.3080 mL 11.5399 mL 23.0798 mL5 mM 0.4616 mL 2.3080 mL 4.6160 mL10 mM 0.2308 mL 1.1540 mL 2.3080 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 RI-2种可逆的 RAD51 抑制剂，IC50 值为 44.17 M，能够特异性地抑制体细胞中的同源重 组修复。IC50 & Target IC50

3、: 44.17 M (RAD51) 1体外研究 RI-2 (7a) is a reversible RAD51 inhibitor, with an IC50 of 44.17 M. RI-2 specifically inhibits homologousrecombination repair in human cells. RI-2 (150 M) induces a significant sensitization of cells 1.1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEPROTOCOLCell Assay 1 HEK2

4、93 cells are plated into 96-well tissue culture plates at a density of 300 cells per well in the presence orabsence of 50 nM mitomycin C (MMC) for 24 hours at 37C, 5% CO2. Media is subsequently replaced withfresh media containing 0.5% DMSO plus RI-2 for an additional 24 hours. RI-2 is then removed,

5、and culturesare allowed to grow to a 50-70% confluence. Average survival from at least three replicates is measuredusing CellGlo reagentor. RI-2 is deemed successful in sensitizing cells to MMC if they generate significantlygreater toxicity in the presence of MMC relative to the absence of MMC. Spec

6、ifically, sensitization is scoredas a “+” when non-overlapping standard errors are observed for at least two pairs of compound doses 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Budke B, et al. An optimized RAD51 inhibitor that disrupts homologous recombination without requiring Michael acceptor reactivity. JMed Chem. 2013 Jan 10;56(1):254-63.McePdfHeightCaution: Product has not been fully validated for medical applications.For research use only.T