HS-1371 - RIP kinase 抑制剂 - 生命科学试剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEHS-1371Cat. No.: HY-114349CAS No.: 2158197-70-5分式: CHNO分量: 384.47作靶点: RIP kinase作通路: Apoptosis储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 16.67 mg/mL (43.36 mM; Need ultrasonic)Mass Sol

2、vent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.6010 mL 13.0049 mL 26.0098 mL5 mM 0.5202 mL 2.6010 mL 5.2020 mL10 mM 0.2601 mL 1.3005 mL 2.6010 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验 请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制澄清的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；澄清的储备液可以根据储存条件，适当保存；以下溶剂前的百分 指该溶

3、剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 1.67 mg/mL (4.34 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 1.67 mg/mL (4.34 mM); Clear solution3. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 1.67 mg/mL (4.34 mM); Clear solution1

4、/2 Master of Small Molecules 您边的抑制剂师www.MedChemEBIOLOGICAL ACTIVITY物活性 HS-1371效，ATP-competitive的受体相互作蛋3 (RIP3)抑制剂，IC50 为 20.8 nM 1。IC50 & Target IC50: 20.8nM (RIP3) 1体外研究 HS-1371 directly binds to RIP3 in an ATP-competitive and time-independent manner. HS-1371 has aninhibitory effect on RIP3 kinase

5、activity in various cell lines 1.HS-1371 (0.1, 1, 5, 10, 20 M, 9 h) can block RIP3 S227 phosphorylation 1.HS-1371 (0.1, 1, 5, 10 M, 24 h) rescues cells from RIP3-dependent necroptotic cell death but not apoptoticcell death 1.Western Blot Analysis 1Cell Line: HT-29 cellsConcentration: 0.1, 1, 5, 10,

6、20 MIncubation Time: 9 hoursResult: Showed an inhibitory effect on S227 auto-phosphorylation of RIP3 1.REFERENCES1. Park HH, et al. HS-1371, a novel kinase inhibitor of RIP3-mediated necroptosis. Exp Mol Med. 2018 Sep 20;50(9):125.McePdfHeightCaution: Product has not been fully validated for medical applications.For research use only.Tel: 400-8