常用缓冲溶液的配制及PH计校正溶液配置方法

1、.技术资料.专业整理.常用缓冲溶液的配制方法1.甘氨酸-盐酸缓冲液(O05mol/L)X毫升0.2mol/L甘氨酸+Y毫升0.2mol/LHCI,再加水稀释至200毫升pHXYpHXY2.05044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分子量=75.07,0.2mol/L甘氨酸溶液含15.01克/升。2.邻苯二甲酸-盐酸缓冲液(005mol/L)X毫升0.2mol/L邻苯二甲冃酸氢钾+0.2mol/LHCl，再加水稀释到20毫升pH(20C)XYpH(20C)XY2.254.0703.251.4702.

2、453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.022邻苯二甲酸氢钾分子量=204.23,0.2mol/L邻苯二甲酸氢溶液含40.85克/升3磷酸氢二钠-柠檬酸缓冲液pH0.2mol/LNa2HPO4(毫升)0.1mol/L柠檬酸(毫升)pH0.2mol/LNa2HPO4(毫升)0.1mol/L柠檬酸(毫升)2.20.4010.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04.1115.896.0

3、12.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55Na2HPO4分子量=14.98,0.2mol/L溶液为28.40克/升。Na2HPO42H2O分子量

4、=178.05,0.2mol/L溶液含35.01克/升。C4H2O7H2O分子量=210.14,0.1mol/L溶液为21.01克/升。4.柠檬酸-氢氧化钠-盐酸缓冲液pH钠离子浓度（mol/L）柠檬酸（克）C6H8O7H2O氢氧化钠（克）NaOH97%盐酸（毫升）HC1（浓）最终体积（升）2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610使用时可以每升中加入1克克酚，右最后pH值有变化，再用少量50%氢氧

5、化钠溶液或浓盐酸调节，冰箱保存。5.柠檬酸-柠F檬酸钠缓冲液（01mol/L）pH0.1mol/L0.1mol/LpH0.1mol/L0.1mol/L柠檬酸柠檬酸钠柠檬酸柠檬酸钠（毫升）（毫升）（毫升）（毫升）3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39.76.61.418.64.89.210.8柠檬酸C6

6、H8O7H2o：分子量210.14,0.1mol/L溶液为21.01克/升。柠檬酸钠Na3C6H5O72H2O:分子量294.12，0.1mol/L溶液为29.41克/毫升。6.乙酸-乙酸钠缓冲液（02mol/L）pH（18C）0.2mol/LNaAc（毫升）0.3mol/LHAc（毫升）pH（18C）0.2mol/LNaAc（毫升）0.3mol/LHAc（毫升）2.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.904.64.90

7、5.105.89.400.60Na2Ac3H2O分子量=136.09,0.2mol/L溶液为27.22克/升。7.磷酸盐缓冲液（1）磷酸氢二钠-磷酸二氢钠缓冲液（0.2）0.2mol/L0.2mol/L0.2mol/L0.2mol/LpHNa2HPO4（毫升）NaH2PO4（毫升）pHNa2HPO4（毫升）NaH2PO4（毫升）5.88.092.07.061.039.05.910.090.07.167.033.06.012.387.77.272.028.06.115.085.07.377.023.06.218.581.57.481.019.06.322.577.57.584.016.06.42

8、6.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.551.08.094.75.36.955.045.0Na2HPO42H2O分子量=178.05,0.2mol/L溶液为85.61克/升。Na2HPO412H2O分子量=358.22,0.2mol/L溶液为71.64克/升。Na2HPO42H2O分子量=156.03,0.2mol/L溶液为31.21克/升。（2）磷酸氢二钠-磷酸二氢钾缓冲液（1/15mol/L）pHmol/L/15Na2HPO4（毫升）mol/L/15KH

9、2PO4（毫升）pHmol/L/15Na2HPO4（毫升）mol/L/15KH2PO4（毫升）4.920.109.907.177.003.005.290.509.507.388.002.005.911.009.007.739.001.006.242.008.008.049.500.506.473.007.008.349.750.256.644.006.008.679.900.106.815.005.008.1810.0006.986.004.00Na2HPO42H2O分子量=178.05，1/15M溶液为11.876克/升。KH2PO4分子量=136.09，1/15M溶液为9.078克/升。8

10、.磷酸二氢钾-氢氧化钠缓冲液（0.05M）X毫升0.2M.PO4+Y毫升0.2NNaOH加水稀释至29毫升pH（20C）X（毫升）Y（毫升）pH（20C）X（毫升）Y（毫升）5.850.3727.052.9636.050.5707.253.5006.250.8607.453.9506.451.2607.654.2806.651.7807.854.5206.852.3658.054.6809.巴比妥钠-盐酸缓冲液（18C）pH0.04M巴比妥钠溶液0.2V盐酸pH0.04M巴比妥钠溶液（毫0.2N盐酸（毫升）升）6.810018.48.41005.217.010017.88.61003.827.

11、210016.78.81002.527.410015.39.01001.657.610013.49.21001.137.810011.479.41000.708.01009.399.61000.358.21007.21100巴比妥钠盐分子量=206.18;0.04M溶液为8.25克/升10.Tris-盐酸缓冲液（0.05M,25C）50毫升0.1M三羟甲基氨基甲烷（Tris）溶液与X毫升0.1N盐酸混匀后，加水稀释至100毫升。pHX（毫升）pHX（毫升）7.1045.78.1026.27.2044.78.2022.97.3043.48.3019.97.4042.08.4017.27.5040

12、.38.5014.77.6038.58.6012.47.7036.68.7010.37.8034.58.808.57.9032.08.907.08.0029.2三羟甲基氨基甲烷（Tris）HOCH2CH2OHCHOCH2NH2分子量=121.14;0.1M溶液为12.114克/升。Tris溶液可从空气中吸收二氧化碳，使用时注意将瓶盖严。11.硼酸-硼砂缓冲液（0.2M硼酸根）pH0.05M硼砂（毫升）0.2M硼砂（毫升）pH0.05M硼砂（毫升）0.2M硼酸（毫升）7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.

13、08.02.0硼砂Na2B4O7H2O,分子量=381.43;0.05M溶液（=0.2M硼酸根）含19.07克/升。硼酸H2BO3，分子量=61.84,0.2M溶液为12.37克/升。硼砂易失去结晶水，必须在带塞的瓶中保存。11硼酸-硼砂缓冲液（0.2M硼酸根）pH0.05M硼砂0.2M硼酸pH0.05M硼砂0.2M硼酸（毫升）（毫升）（毫升）（毫升）7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂Na2B4O710H2O,分子量=381.43;0.05M溶液（=0.2M硼酸根）含19.07克/

14、升。硼酸H2BO3，分子量=61.84,0.2M溶液为12.37克/升。硼砂易失去结晶水，必须在带塞的瓶中保存。12.甘氨酸-氢氧化钠缓冲液（0.05M）X毫升0.2M甘氨酸+Y毫升0.2NnaOH加水稀释至200毫升pHXYpHXY8.6504.09.65022.48.8506.09.85027.29.0508.810.05032.09.25012.010.45038.69.45016.810.65045.5甘氨酸分子量=75.07;0.2M溶液含15.01克/升。13.硼砂-氢氧化钠缓冲液（0.05M硼酸根）X毫升0.05M硼砂+Y毫升0.2NNaOH加水稀释至200毫升pHXYpHXY9

15、.3506.09.85034.09.45011.010.05043.09.65023.010.15046.0硼砂Na2B4O710H2O,分子量=381.43;0.05M溶液为19.07克/升。14碳酸钠-碳酸氢钠缓冲液（0.1M）Ca2+、Mg2+存在时不得使用pH0.1MNa2CO3（毫升）0.1MN2HCO3（毫升）20C37C9.168.77199.409.12289.519.40379.789.50469.909.725510.149.906410.2810.087310.5310.288210.8310.5791Na2CO210H2O分子量=286.2;0.1M溶液为28.62克/升。N2HCO3分子量=84.0;0.1M溶液为8.40克/升。15.“PBS”缓冲液PH7.67.47.27.0H2O100010001000100mlNaCl8.58.58.58.5gNa2HPO42.22.22.22.2gNaH2PO40.10.20.30.4gPH计校正溶液配置的标准方法pH4，邻苯二甲酸氢钾标准缓冲液：精密称取在1155C干燥23小时的邻苯二甲酸氢钾KHC8H40410.12g,加水使溶解并稀释至10