下载本文档
版权说明:本文档由用户提供并上传,收益归属内容提供方,若内容存在侵权,请进行举报或认领
文档简介
1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEDeltoninCat. No.: HY-N2283CAS No.: 55659-75-1分式: CHO分量: 885.04作靶点: ERK; Akt作通路: MAPK/ERK Pathway; Stem Cell/Wnt; PI3K/Akt/mTOR储存式: 4C, protect from light* In solvent : -80C, 6 months; -20C, 1 month (protect fromlight)BIOLOGICAL
2、ACTIVITY物活性 Deltonin是从盾叶薯蓣中得到的甾体皂苷,能够抑制 ERK1/2 和 AKT 的活化,具有抗肿瘤的活性。IC50 & Target ERK1 ERK2 Akt体外研究 Deltonin inhibits ERK1/2 and AKT activation. Deltonin (0.5-8 M) significantly inhibits the proliferation ofseveral colon cancer cell lines including SW480, SW620, LOVO and C26, with IC50s of 1.3, 1.29,
3、2.11 and1.22 M. Deltonin also causes G2-M phase arrest and induces apoptosis in C26 cells 1. Deltonin (0-5 M)decreases the phosphorylation of AKT and ERK1/2 levels in MDAMB231 cells. Deltonin (0.5-8M) alsodose-dependently inhibits the proliferation, and causes apoptosis with ROS production in MDAMB2
4、31 cells2.体内研究 Deltonin (20 and 40 mg/kg, p.o.) reduces the tumor growth of 37.7% and 56.7%, and causes a 50-daysurvival rate of 60% and 50%, respectively, in mice. Deltonin also inhibits tumor angiogenesis in mice 1.PROTOCOLCell Assay 1 The cytotoxicity of Deltonin on colon cancer cells is determin
5、ed by the MTT assay. Cells are seeded at adensity of 3 103 per well in 96-well plates. After incubation overnight, cells are treated with Deltonin invarious concentrations (0.5, 1, 2, 4, 8 M), and carrier DMSO (50) is calculated 1.MCE has not independently confirmed the accuracy of these methods. Th
6、ey are for reference only.Animal Mice 11/2 Master of Small Molecules 您边的抑制剂师www.MedChemEAdministration 1 Briefly, 5 105 C26 cells are s.c. injected into 4- to 6-wk-old male BALB/c mice. The mice are randomlydivided into four groups of 10 mice. One week after C26 implantation, the treatment groups re
7、ceive their firstdoses of Deltonin dissolved in a vehicle solution of DMSO (. Briefly, Deltonin is given via oral administrationto tumor-bearing mice at 10, 20 and 40 mg/kg every day for 3 weeks (between day 8 and 28). In parallel, thecontrol group receives the vehicle (DMSO, 0.1%) in saline solutio
8、n. The same quantity of saline solutioncontaining DMSO is used in these groups. General clinical observations of the mice, including determinationof body weight and tumor growth, are made twice weekly. To determine tumor size, two perpendiculardiameters of the tumor in centimeters is measured by cal
9、ipers. Tumor volume is estimated using the formula0.52 a b2, where “a” is the long diameter and “b” is the short diameter. The mice are sacrificed when theybecome moribund, and the sacrifice date is recorded to calculate the survival time. To detect the apoptosisprotein expression and microvessel de
10、nsity, tumor tissues are removed, fixed in 10% formalin or not andsnap-frozen in liquid nitrogen immediately 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Tong QY, et al. Deltonin, a steroidal saponin, inhibits colon cancer cell growth
11、in vitro and tumor growth in vivo via induction of apoptosisand antiangiogenesis. Cell Physiol Biochem. 2011;27(3-4):233-42.2. Zhang S, et al. Deltonin induces apoptosis in MDAMB231 hum an breast cancer cells via reactive oxygen speciesm ediated m itochondrialdysfunction and ERK/AKT signaling pathways. Mol Med Rep. 2013 Mar;7(3):1038-44.McePdfHeightCaution: Product has no
温馨提示
- 1. 本站所有资源如无特殊说明,都需要本地电脑安装OFFICE2007和PDF阅读器。图纸软件为CAD,CAXA,PROE,UG,SolidWorks等.压缩文件请下载最新的WinRAR软件解压。
- 2. 本站的文档不包含任何第三方提供的附件图纸等,如果需要附件,请联系上传者。文件的所有权益归上传用户所有。
- 3. 本站RAR压缩包中若带图纸,网页内容里面会有图纸预览,若没有图纸预览就没有图纸。
- 4. 未经权益所有人同意不得将文件中的内容挪作商业或盈利用途。
- 5. 人人文库网仅提供信息存储空间,仅对用户上传内容的表现方式做保护处理,对用户上传分享的文档内容本身不做任何修改或编辑,并不能对任何下载内容负责。
- 6. 下载文件中如有侵权或不适当内容,请与我们联系,我们立即纠正。
- 7. 本站不保证下载资源的准确性、安全性和完整性, 同时也不承担用户因使用这些下载资源对自己和他人造成任何形式的伤害或损失。
最新文档
- 有关春节安全通知五篇
- 毕业财务实习报告
- 2024年计量仪器器具项目发展计划
- 2024年电子式金属、非金属试验机合作协议书
- 2024年输注延长管项目发展计划
- 有关新学期学习计划模板合集9篇
- 有关高一新学期学习计划3篇
- 2024年专科疾病防治服务项目合作计划书
- 2024年无损检测仪器合作协议书
- 2024年应急救生系统项目建议书
- 骨科运用PDCA循环提高深静脉血栓中高危风险患者预防措施的落实率品管圈QCC持续质量改进成果汇报
- 槟榔栽培实用技术
- 五年级上综合实践活动第1课探究纸“三态”教学课件海天版(深圳用)
- 无人机的控制系统设计
- 拆除台阶施工方案
- 2023年河南省普通高校专升本公共英语真题(试卷+答案)
- 领导班子议事制度
- 2023年10月自考00534外国文学作品选试题及答案含评分标准
- 克服厌学情绪 班会教学设计
- 复合锂基润滑脂和脲基润滑脂成脂机理的对比研究
- 搪玻璃反应釜维护检修规程2016
评论
0/150
提交评论