ISO-1-MIF-Antagonist-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEISO-1Cat. No.: HY-16692CAS No.: 478336-92-4Synonyms: MIF Antagonist分式: CHNO分量: 235.24作靶点: Others作通路: Others储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 36 mg/mL (153.04 mM)* means solubl

2、e, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 4.2510 mL 21.2549 mL 42.5098 mL5 mM 0.8502 mL 4.2510 mL 8.5020 mL10 mM 0.4251 mL 2.1255 mL 4.2510 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 ISO-1巨噬细胞移动抑制因(MIF) 拮抗剂，IC50 值为 7 M。IC50 & Target IC50:

3、7M (MIF) 1体外研究ISO-1 (0.1-20 M; 16 hours) has a slight inhibitory effect on Cox-2 secretion without the addition of1/3 Master of Small Molecules 您边的抑制剂师www.MedChemErecombinant MIF 1.Western Blot Analysis 1Cell Line: RAW 264.7 macrophage cellsConcentration: 0.1M，1M，10M，20MIncubation Time: 16 hoursResu

4、lt: Suppressed Cox-2 secretion.体内研究 ISO-1 (injected intraperitoneally; 3.5-35 mg/kg; twice daily; 2 weeks) improves the survival rate from lethalendotoxemia and shows the anti-inflammatory effect 2.ISO-1 (intraperitoneal injection; 35 mg/kg; twicedaily; 3 days) causes a significant reduction in aver

5、age implant size and decreases Flk1 expression inimplants 3.Animal Model: C57Bl/6 MIF+/+ and MIF/ mice 2Dosage: 3.5-35 mg/kgAdministration: Injected intraperitoneally; 3.5-35 mg/kg; twice a day ; 2 weeksResult: Was protective against lethal sepsis.Animal Model: Female C57BL/6-Tg(ACTB-EGFP)1Osb/J mic

6、e 3Dosage: 35 mg/kgAdministration: Intraperitoneal injection; 35 mg/kg; twice daily; 3 daysResult: Reduced average endometriotic implant size.户使本产品发表的科研献 J Neuroinflammation. 2018 Oct 19;15(1):291. Mol Med Rep. 2019 Jul 9.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Lubetsky

7、 JB, et al. The tautomerase active site of macrophage migration inhibitory factor is a potential target for discovery of novel anti-inflammatory agents. J Biol Chem. 2002 Jul 12;277(28):24976-82.2. Al-Abed Y, et al. ISO-1 binding to the tautomerase active site of MIF inhibits its pro-inflammatory ac

8、tivity and increases survival in severesepsis. J Biol Chem. 2005 Nov 4;280(44):36541-4.2/3 Master of Small Molecules 您边的抑制剂师www.MedChemE3. Nothnick WB, et al. Inhibition of macrophage migration inhibitory factor reduces endometriotic implant size in mice with experimentallyinduced disease. J Endometr. 2011 Sep 30;3(3):135-142.McePdfHeightCaution: Product has not been fully validated for medical applicatio