Pentostatin-CI-825-DataSheet-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEPentostatinCat. No.: HY-A0006CAS No.: 53910-25-1Synonyms: CI-825; Deoxycoformycin分式: CHNO分量: 268.27作靶点: Adenosine Deaminase作通路: Metabolic Enzyme/Protease储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据

2、体外实验 DMSO : 50 mg/mL (186.38 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 3.7276 mL 18.6379 mL 37.2759 mL5 mM 0.7455 mL 3.7276 mL 7.4552 mL10 mM 0.3728 mL 1.8638 mL 3.7276 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制

3、澄清的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；澄清的储备液可以根据储存条件，适当保存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (9.32 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (9.32 mM); Clear solution1/3 Mas

4、ter of Small Molecules 您边的抑制剂师www.MedChemE3. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (9.32 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 Pentostatin种效，不可逆的腺苷脱氨酶抑制剂，Ki 值为 2.5 pM。IC50 & Target Ki: 2.5 pM (adenosine deaminase)体内研究 In ECP and Pentostatin (4 mg/m2, i.v.) treatment, all dogs dev

5、elop granulocytopenia with 1. Pentostatin (2mg/kg) in combination with cordycepin (2 mg/kg) is 100% effective in the T. evansi-infected mice. There is anincrease in levels of some biochemical parameters, especially on liver enzymes, which are accompanied byhistological lesions in the liver and kidne

6、ys. Pentostatin individually has no curative effect on infected groups2.PROTOCOLAnimal All recipient dogs are conditioned for transplantation by 920 cGy TBI at 7 cGy/minute using a linearAdministration 1 accelerator. Dogs in group A1receive ECP administered on days 2 and 1 with TBI on day 0 and dogs

7、 ingroup A2receive ECP on days 6 and 5, intravenous (IV) infusion of pentostatin at a dose of 4 mg/m2 ondays 4 and 3, and TBI on day 0. Donor marrow cells from DLA-nonidentical donors are aspirated undergeneral anesthesia through needles inserted into humeri and femora and stored in heparinized tiss

8、ue culturemedium at 4C for no more than 6 hours. Within 4 hours of TBI, harvested marrow cells are infused IV intorecipients at a median dose of 2.9 (range, 1.9 to 6.1) 108 total nucleated cells (TNC)/kg. The day of marrowgrafting is designated as day 0. In addition to marrow graft, recipients are g

9、iven IV infusions of peripheralblood buffy coat cells obtained by leukapheresis from the marrow donor on days 1 and 2, at a median doseof 2.3 (range, 1.2 to 6.9) 108 TNC/kg to ensure consistent hematopoietic engraftment. MTX, at a dose of0.4 mg/kg intravenously is used as postgrafting immunosuppress

10、ion and administered on days +1, +3, +6and +11, then weekly thereafter until day 102.MCE has not independently confirmed the accuracy of these methods. They are for reference only.户使本产品发表的科研献 J Nanobiotechnology. 2019 Mar 29;17(1):45. J Neurochem. 2019 May 23. Virulence. 2018;9(1):1509-1520.See more

11、 customer validations on HYPERLINK / www.MedChemEREFERENCES2/3 Master of Small Molecules 您边的抑制剂师www.MedChemE1. Bethge WA, et al. Extracorporeal photopheresis combined with pentostatin in the conditioning regimen for canine hematopoietic celltransplantation does not prevent GVHD. Bone Marrow Transplant. 2014 Sep;49(9):1198-204.2. Dalla Rosa L, et al. Cordycepin (3-deoxyadenosine) pentostatin (deoxycoformycin) combination treatment of mice experimentallyinfected with Trypanosoma evansi. Parasitology. 2013 Apr;140(5):663-71.McePdfHeightCaution: Produ