黄芩苷作用机制 - Medchemexpress - MCE中国

1、Product Data SheetBaicalinCat. No.: HY-N0197CAS No.: 21967-41-9分式: CHO分量: 446.36作靶点: NF-B; Autophagy; HIV作通路: NF-B; Autophagy; Anti-infection储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 100 mg/mL (224.03 mM)H2O : 0.1 mg/mL (insoluble)* means soluble, but sat

2、uration unknown.SolventMass1 mg 5 mg 10 mgConcentration制备储备液1 mM 2.2403 mL 11.2017 mL 22.4034 mL5 mM 0.4481 mL 2.2403 mL 4.4807 mL10 mM 0.2240 mL 1.1202 mL 2.2403 mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存式和期限：-80C, 6 months; -20C, 1 month。-80C 储存时，请在 6 个内使，-20C 储存时，请在 1 个内使。体内实验

3、请根据您的实验动物和给药式选择适当的溶解案。以下溶解案都请先按照 In Vitro 式配制澄清的储备液，再依次添加助溶剂：为保证实验结果的可靠性，澄 的储备液可以根据储存条件，适当保存；体内实验的作液，建议您现现配，当天使； 以下溶剂前显的百分 指该溶剂在您配制终溶液中的体积占；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的式助溶1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (5.60 mM); Clear solution此案可获得 2.5 mg/mL (5.60 mM，

4、饱和度未知) 的澄清溶液。以 1 mL 作液为例，取 100 L 25.0 mg/mL 的澄 DMSO 储备液加到 400 L PEG300 中，混合均匀；向上述体系中加50 L Tween-80，混合均匀；然后继续加 450 L 理盐定容 1 mL。2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (5.60 mM); Clear solutionPage 1 of 2 www.MedChemE此案可获得 2.5 mg/mL (5.60 mM，饱和度未知) 的澄清溶液。以 1 mL 作液为例，取 10

5、0 L 25.0 mg/mL 的澄 DMSO 储备液加到 900 L 20% 的 SBE-CD 理盐溶液中，混合均匀。BIOLOGICAL ACTIVITY物活性 Baicalin种从 Scutellaria baicalensis 中分离出来的类黄酮糖苷。Baicalin 降低 NF-B 表达。IC & Target NF-B Autophagy体外研究 Baicalin protects against ischemia-reperfusion injury (IRI) by altering the production of various mediators, includingre

6、active oxygen species (ROS), Toll-like receptor (TLR)2 and TLR4, NF-B, Bax, and Bcl-2. Baicalin treatment inhibitsthe increased expression of the proinflammatory cytokines TLR2/4, MyD88, p-NF-B, and p- IB, as well as increasethe expression of IB protein, an NF-B inhibitor, with the degree of inhibit

7、ion positively related to the dosage ofBaicalin1. Cell viability is determined by MTT assay. Compared with control cells, cell viability is significantlydecreased in SH-SY5Y cells treated with thrombin. Pre-treatment with Baicalin (5, 10, 20 M) increases cell viability ina dose-dependent manner comp

8、ared with cells treated thrombin alone2.体内研究 Baicalin pretreatment dose-dependently protects against a loss of renal function, with the two higher doses (10 and100 mg/kg) significantly decreasing Scr and blood urea nitrogen (BUN) concentrations. Tissue injury, as assessed using a 0-3 point scoring s

9、ystem, is lower for the Baicalin treated groups than for the ischemia-reperfusion (IR)+salinegroup. Compared with the sham group, malondialdehyde (MDA) content is only slightly up-regulated and the SODactivity is only slightly down-regulated in rats treated with 10 and 100 mg/kg Baicalin, indicating

10、 that Baicalinabrogates the increase in oxidative stress following reperfusion1.PROTOCOLCell Assay 2 SH-SY5Y cell lines are cultured in RPMI-1640 medium supplemented with 15% fetal bovine serum at 37C in an airatmosphere containing 95% air and 5% CO2 with a saturated humidity. Upon a confluence of 6

11、070%, the SH-SY5Ycells are divided into: (i) control group, incubated in RPMI-1640 medium; (ii) thrombin group, which is subject tothrombin induction (40 U/L) for 6 h based on our pre-experiment; and (iii) Baicalin groups, which are treated byBaicalin (5 M, 10 M, or 20 M) for 2 h before induction of

12、 thrombin. Cell viability is measured using MTT assay.Briefly, 15 L of the MTT solution (5 mg/mL) is added to each well and incubated for 4 h at 37C. After removing thesupernatant, 80 L DMSO are added into each well. The absorbance is measured at 492 nm using a microplatereader. All experiments are

13、performed in triplicate2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Rats1Administration 1 Male Wistar rats weighing 200-250 g are used. Rats are randomly divided into five groups of six rats each: (i) sham group; (ii) IR+saline group; (iii)

14、IR+Baicalin (1 mg/kg) group; (iv) IR+Baicalin (10 mg/kg) group; and (v) IR+Baicalin(100 mg/kg) group. Renal IRI is induced by clamping the left renal artery for 45 min plus a right nephrectomy. Ratsare anesthetized through an intraperitoneal injection of pentobarbital sodium (40 mg/kg body weight).

15、After amedian abdominal incision, the left renal arteries are clamped for 45 min with serrefine. After clamp removal,adequate restoration of blood flow is checked before abdominal closure. The right kidney is then removed. Sham-operated animals underwent the same surgical procedure without clamping1

16、.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Page 2 of 3 www.MedChemE户使本产品发表的科研献 Oral Dis. 2019 Aug 8.See more customer validations on HYPERLINK www.MedChemE www.MedChemEREFERENCES1. Lin M, et al. The protective effect of Baicalin against renal ischemia-reperfusion injury through inhibition of inflammation and apoptosis. BMCComplement Altern Med. 2014 Jan 13;14:19.2. Ju XN, et al. Baicalin protects against thrombin induced cell injury in SH-SY5Y cells. Int J Clin Exp Pa