烟酰胺作用机制 - Medchemexpress - MCE中国

1、Product Data SheetNicotinamideCat. No.: HY-B0150CAS No.: 98-92-0分式: CHNO分量: 122.12作靶点: Endogenous Metabolite; Sirtuin作通路: Metabolic Enzyme/Protease; Cell Cycle/DNA Damage; Epigenetics储存式: 4C, protect from light* In solvent : -80C, 6 months; -20C, 1 month (protect from light)溶解性数据体外实验 DMSO : 100 mg/m

2、L (818.87 mM)H2O : 50 mg/mL (409.43 mM)* means soluble, but saturation unknown.SolventMass1 mg 5 mg 10 mgConcentration制备储备液1 mM 8.1887 mL 40.9433 mL 81.8867 mL5 mM 1.6377 mL 8.1887 mL 16.3773 mL10 mM 0.8189 mL 4.0943 mL 8.1887 mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存式和期限：-80C,

3、6 months; -20C, 1 month (protect from light)。-80C 储存时，请在 6 个内使，-20C 储存时，请在 1 个内使。体内实验请根据您的实验动物和给药式选择适当的溶解案。以下溶解案都请先按照 In Vitro 式配制澄清的储备液，再依次添加助溶剂：为保证实验结果的可靠性，澄 的储备液可以根据储存条件，适当保存；体内实验的作液，建议您现现配，当天使； 以下溶剂前显的百分 指该溶剂在您配制终溶液中的体积占；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的式助溶1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-

4、80 45% salineSolubility: 2.5 mg/mL (20.47 mM); Clear solution此案可获得 2.5 mg/mL (20.47 mM，饱和度未知) 的澄清溶液。以 1 mL 作液为例，取 100 L 25.0 mg/mL 的澄 DMSO 储备液加到 400 L PEG300 中，混合均匀；向上述体系中加50 L Tween-80，混合均匀；然后继续加 450 L 理盐定容 1 mL。2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (20.47 mM); Clea

5、r solution此案可获得 2.5 mg/mL (20.47 mM，饱和度未知) 的澄清溶液。Page 1 of 2 www.MedChemE以 1 mL 作液为例，取 100 L 25.0 mg/mL 的澄均匀。DMSO 储备液加到 900 L 20% 的 SBE-CD 理盐溶液中，混合3. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (20.47 mM); Clear solution此案可获得 2.5 mg/mL (20.47 mM，饱和度未知) 的澄 溶液，此案不适于实验周 期在半个以上的实验。以 1 mL 作液为例，

6、取 100 L 25.0 mg/mL 的澄 DMSO 储备液加到 900 L 油中，混合均匀。BIOLOGICAL ACTIVITY物活性 Nicotinamide 种B3维素，通过促进NAD +氧化还原稳态和提供NAD +作为催化氧化还原反应的类酶的底物，在细胞理学中发挥重 作。 烟酰胺是SIRT1 的抑制剂。IC & Target PARP-1 Human Endogenous Metabolite体外研究 Pretreatment with the poly (ADP-ribose) polymerase (PARP) inhibitor nicotinamide is able to

7、prevent HCN2 cell death.When nicotinamide is added prior to t-BuOOH, it is able to prevent neuronal cell death and inhibit apoptosis.Nicotinamide-pretreated neurons have higher expression levels of inhibitors of apoptosis (IAP) genes1. Nicotinamideinhibits vasoconstriction by ET. Nicotinamide also a

8、lleviates oxidative stress, which exacerbates PE and FGR3.体内研究 Normal and streptozotocin-nicotinamide induced adult male diabetic rats receive quercetin (10, 25 and 50mg/kg/bw) orally, and cause significant decrease in FBG and cardiac injury marker levels with increased in insulinlevels2. Nicotinami

9、de improves maternal hypertension, proteinuria, and glomerular endotheliosis in RUPP mice.Moreover, nicotinamide prolongs pregnancies, and improves survival and growth of the embryos in RUPP PE mice3.PROTOCOLAnimal DM is induced via a single intraperitoneal (i.p) injection of nicotinamide (110 mg/kg

10、/body weight) dissolved inAdministration 2 normal saline 15 min prior to streptozotocin (STZ) (55 mg/kg/body weight) injection, which is dissolved in a freshlyprepared 0.1mol/Lcitrate buffer (pH 4.5). These injections are given following an overnight fast. Control rats (n=6) areinjected with the sam

11、e amount of solvent. In order to prevent hypoglycemia in the first 24 h following STZ injection,rats are allowed to have free access to water with 5% dextrose (D5W). Three days after STZ-nicotinamide injection,rats with FBG levels greater than 7.0 mM are considered as diabetic.MCE has not independen

12、tly confirmed the accuracy of these methods. They are for reference only.户使本产品发表的科研献 Autophagy. 2020 Jan 27:1-15. Theranostics. 2019 Apr 13;9(9):2424-2438. Oncogene. 2020 Jan 23. Hum Reprod. 2020 Mar 6. pii: dez296. Hum Reprod. 2019 Jun 13. pii: dez068.See more customer validations on HYPERLINK www.

13、MedChemE www.MedChemEPage 2 of 3 www.MedChemEREFERENCES1. Bhansali SG, et al. Nicotinamide prevents apoptosis in human cortical neuronal cells. Toxicol Mech Methods. 2006;16(4):173-80.2. Roslan J, et al. Quercetin ameliorates oxidative stress, inflammation and apoptosis in the heart of streptozotoci

14、n-nicotinamide-induced adult malediabetic rats. Biomed Pharmacother. 2016 Dec 24;86:570-5823. Fushima T, et al. Nicotinamide ameliorates a preeclampsia-like condition in mice with reduced uterine perfusion pressure. Am J Physiol Renal Physiol.2016 Dec 7:ajprenal.00501.20164. Suzuki E, et al. Protect

15、ive effect of nicotinamide against poly(ADP-ribose) polymerase-1-mediated astrocyte death depends on its transporter-mediateduptake. Life Sci. 2010 Apr 24;86(17-18):676-82.5. Peck B, et al. SIRT inhibitors induce cell death and p53 acetylation through targeting both SIRT1 and SIRT2. Mol Cancer Ther. 2010 Apr;9(4):844