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1、Product Data SheetMangiferinCat. No.: HY-N0290CAS No.: 4773-96-0分式: CHO分量: 422.34作靶点: NF-B; Keap1-Nrf2; Apoptosis作通路: NF-B; Apoptosis储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 50 mg/mL (118.39 mM; Need ultrasonic)SolventMass1 mg 5 mg 10 mgConcentration制备储备
2、液1 mM 2.3678 mL 11.8388 mL 23.6776 mL5 mM 0.4736 mL 2.3678 mL 4.7355 mL10 mM 0.2368 mL 1.1839 mL 2.3678 mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;旦配成溶液,请分装保存,避免反复冻融造成的产品失效。储备液的保存式和期限:-80C, 6 months; -20C, 1 month。-80C 储存时,请在 6 个内使,-20C 储存时,请在 1 个内使。体内实验请根据您的实验动物和给药式选择适当的溶解案。以下溶解案都请先按照 In Vitro 式配制澄清的储备液,再依次添加助溶
3、剂:为保证实验结果的可靠性,澄 的储备液可以根据储存条件,适当保存;体内实验的作液,建议您现现配,当天使; 以下溶剂前显的百分 指该溶剂在您配制终溶液中的体积占;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的式助溶1. 请依序添加每种溶剂: 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (5.92 mM); Clear solution此案可获得 2.5 mg/mL (5.92 mM,饱和度未知) 的澄清溶液。以 1 mL 作液为例,取 100 L 25.0 mg/mL 的澄 DMSO 储备液加到 4
4、00 L PEG300 中,混合均匀;向上述体系中加50 L Tween-80,混合均匀;然后继续加 450 L 理盐定容 1 mL。2. 请依序添加每种溶剂: 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (5.92 mM); Clear solution此案可获得 2.5 mg/mL (5.92 mM,饱和度未知) 的澄清溶液。Page 1 of 2 www.MedChemE以 1 mL 作液为例,取 100 L 25.0 mg/mL 的澄均匀。DMSO 储备液加到 900 L 20% 的 SBE-CD 理盐溶液中,混合3
5、. 请依序添加每种溶剂: 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (5.92 mM); Clear solution此案可获得 2.5 mg/mL (5.92 mM,饱和度未知) 的澄 溶液,此案不适于实验周 期在半个以上的实验。以 1 mL 作液为例,取 100 L 25.0 mg/mL 的澄 DMSO 储备液加到 900 L 油中,混合均匀。BIOLOGICAL ACTIVITY物活性 Mangiferin种 Nrf2 激活剂。Mangiferin 抑制 NF-B 亚基p65 和 p50 的核移位。IC & Target p65 p50 Nrf
6、2体外研究 Mangiferin is glucosylxanthone extracted from plants of the Anacardiaceae and Gentianaceae families. Mangiferin (50M) significantly increases Nrf2 protein accumulation in HL-60 cells, particularly in the nucleus. Mangiferin alsoenhances the binding of Nrf2 to an antioxidant response element (A
7、RE), significantly up-regulates NAD(P)H:quinoneoxidoreductase 1 (NQO1) expression and reduces intracellular ROS in HL60 cells. Mangiferin alone dose-dependentlyinhibits the proliferation of HL-60 cells. In mononuclear cells (MNCs), Mangiferin significantly relieves oxidative stress,but attenuates et
8、oposide-induced cytotoxicity1. Mangiferin is a natural phytopolyphenol that exhibits variouspharmacological properties. Mangiferin is present in several plant species such as Mangifera indica, Iris unguicularis,and Anemarrhena asphodeloides. Mangiferin downregulates TNF-induced MMP-9 mRNA and protei
9、n expressionby suppressing NF-B activity, consequently suppressing the invasion of LNCaP cells.To assess whether Mangiferininfluences the viability of LNCaP cells, MTT assay is performed 24 h after treatment with different concentrations ofMangiferin in the presence or absence of TNF- in serum and s
10、erum-free conditions. There are no cytotoxic evidentin LNCaP cells treated with up to 400 M of Mangiferin alone under both serum and serum-free conditions.Additionally, in the presence of TNF- (20 ng/mL), Mangiferin does not affect cell viability. Mangiferin (400 M)treatment significantly decreases
11、the NF-B luciferase activity in TNF-stimulated LNCaP cells. Pretreatment withMangiferin (400 M) for 1 h significantly decreases TNF-induced NF-B activity. The RT-PCR shows that Mangiferin(400 M) significantly suppresses the TNF-induced MMP-9 expression in LNCaP cells2.PROTOCOLCell Assay 2 LNCaP cell
12、s are cultured at 37C in 5% CO2 in RPMI supplemented with 10% FBS and antibiotics. Cell viability isdetermined by an MTT assay. Briefly, LNCaP cells (1105 cells/mL) are plated onto 24 well plates and incubatedovernight in serum and serum-free RPMI media. The cells are treated with the indicated conc
13、entrations of Mangiferin(100, 200, and 400 M) for 1 h and then stimulated with TNF- (20 ng/mL) for 24 h. Then, the cells are incubatedwith a solution of 0.5 mg/mL MTT and incubation for 45 min at 37C and 5% CO2. Supernatant is removed and theformation of formazan is observed by monitoring the signal
14、 at 540 nm using a microplate reader2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.户使本产品发表的科研献 Biochem Biophys Res Commun. 2018 Sep 3;503(1):297-303.See more customer validations on HYPERLINK www.MedChemE www.MedChemEPage 2 of 3 www.MedChemEREFERENCE
15、S1. Zhang BP, et al. Mangiferin activates Nrf2-antioxidant response element signaling without reducing the sensitivity to etoposide of human myeloidleukemia cells in vitro. Acta Pharmacol Sin. 2014 Feb;35(2):257-66.2. Dilshara MG, et al. Mangiferin inhibits tumor necrosis factor-induced matrix metalloproteinase-9 expression and cellular invasion by suppressingnuclear factor-B activity. BMB Rep. 2015 Oct;48(10
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