去氧胆酸作用机制 - Medchemexpress - MCE中国

1、Product Data SheetDeoxycholic acidCat. No.: HY-N0593CAS No.: 83-44-3分式: CHO分量: 392.57作靶点: GPCR19; Endogenous Metabolite作通路: GPCR/G Protein; Metabolic Enzyme/Protease储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 100 mg/mL (254.73 mM)H2O : 0.1 mg/mL (insoluble)

2、* means soluble, but saturation unknown.SolventMass1 mg 5 mg 10 mgConcentration制备储备液1 mM 2.5473 mL 12.7366 mL 25.4732 mL5 mM 0.5095 mL 2.5473 mL 5.0946 mL10 mM 0.2547 mL 1.2737 mL 2.5473 mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存式和期限：-80C, 6 months; -20C, 1 month。-80C 储存时，请在 6 个内

3、使，-20C 储存时，请在 1 个内使。体内实验请根据您的实验动物和给药式选择适当的溶解案。以下溶解案都请先按照 In Vitro 式配制澄清的储备液，再依次添加助溶剂：为保证实验结果的可靠性，澄 的储备液可以根据储存条件，适当保存；体内实验的作液，建议您现现配，当天使； 以下溶剂前显的百分 指该溶剂在您配制终溶液中的体积占；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的式助溶1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (6.37 mM); Suspended solut

4、ion; Need ultrasonic此案可获得 2.5 mg/mL (6.37 mM) 的均匀悬浊液，悬浊液可于服和腹腔注射。以 1 mL 作液为例，取 100 L 25.0 mg/mL 的澄 DMSO 储备液加到 400 L PEG300 中，混合均匀；向上述体系中加50 L Tween-80，混合均匀；然后继续加 450 L 理盐定容 1 mL。2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (6.37 mM); Clear solutionPage 1 of 2 www.MedChemE此案

5、可获得 2.5 mg/mL (6.37 mM，饱和度未知) 的澄清溶液。以 1 mL 作液为例，取 100 L 25.0 mg/mL 的澄 DMSO 储备液加到 900 L 20% 的 SBE-CD 理盐溶液中，混合均匀。3. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (6.37 mM); Clear solution此案可获得 2.5 mg/mL (6.37 mM，饱和度未知) 的澄 溶液，此案不适于实验周 期在半个以上的实验。以 1 mL 作液为例，取 100 L 25.0 mg/mL 的澄 DMSO 储备液加到 900 L

6、 油中，混合均匀。BIOLOGICAL ACTIVITY物活性 Deoxycholic acid 激活 G 蛋偶联胆汁酸受体TGR5。IC & Target Human Endogenous Metabolite体外研究 Deoxycholic acid (DCA) and chenoDeoxycholic acid (CDCA), as the common ingredients of duodenal reflux, actsynergistically in many physiological and pathological processes. The cells are rep

7、eatedly exposed to 100 M CDCAand Deoxycholic acid at pH 5.5 for up to 120 min. To simulate chronic local recurrent disease in vitro, the gastriccancer cell line MGC803 is exposed to acidified medium (pH 5.5) containing 100 M Deoxycholic acid and CDCA. Anuntreated log-growth MGC803 cell line is gener

8、ated to be used as a control in normal pH media. After daily 10 minexposure to the acidified bile acids for 60 weeks, MGC803-resistant cells are able to survive and proliferate after 120min exposure2.PROTOCOLCell Assay 2 MGC803 cells are cultured in Roswell Park Memorial Institute media supplemented

9、 with 10% fetal calf serum and 100U/mL Penicillin and 100 mg/mL Streptomycin. To generate MGC803-resistant cells, the pH value of the MGC803culture medium is adjusted to the experimental conditions using the hydrochloric acid (A). The bile acids GCDA andDeoxycholic acid are diluted to optimal workin

10、g concentrations of 100 M (B) with culture medium, and the overallpH (A+B) is adjusted to pH 5.5, simulating the gastric environment. Initially, MGC803 cells are chronically exposed toacidified medium with bile acids (A+B) for 10 min every 24 h. The experimental time and conditions are optimized ino

11、ur preliminary experiments, which show that 10 min is enough and does not result in cell damage. This procedure isrepeated and it takes 60 weeks for the MGC803 cells to survive and proliferate under the exposure of A+B for 120min. Control untreated cells are cultured in neutral RPMI medium at pH 7.4

12、 in parallel to the resistant cells for 60weeks. The morphological changes in MGC803 cells exposed to acidified bile acids (A+B) are documented at 30 and60 weeks2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.户使本产品发表的科研献 Cell Res. 2019 Mar;29(3):193-2

13、05. Nat Cell Biol. 2018 Oct;20(10):1145-1158. Microbiome. 2019 Mar 20;7(1):43. Cancer Cell Int. 2019 Jan 31;19:24.Page 2 of 3 www.MedChemE Laurea Magistrale in Biomedical Engineering, Politecnico di Milano. 2019 Jun.See more customer validations on HYPERLINK www.MedChemE www.MedChemEREFERENCES1. Som

14、m E, et al. -Klotho deficiency protects against obesity through a crosstalk between liver, microbiota, and brown adipose tissue. JCI Insight. 2017 Apr20;2(8). pii: 91809.2. Wang X, et al. Acidified bile acids enhance tumor progression and telomerase activity of gastric cancer in micedependent on c-Myc expression. CancerMed. 2017 Apr;6(4):788-797.McePdfHei