柚皮苷作用机制 - Medchemexpress - MCE中国

1、Product Data SheetNaringinCat. No.: HY-N0153CAS No.: 10236-47-2分式: CHO分量: 580.53作靶点: Cytochrome P450; Autophagy; Mitophagy; Endogenous Metabolite作通路: Metabolic Enzyme/Protease; Autophagy储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 1 mg/mL (1.72 mM; Need ultr

2、asonic)Ethanol : 1 mg/mL (insoluble)H2O : 1 mg/mL (1.72 mM; ultrasonic and warming and heat to 80C)SolventMass1 mg 5 mg 10 mgConcentration制备储备液1 mM 1.7226 mL 8.6128 mL 17.2256 mL5 mM - - -10 mM - - -请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存式和期限：-80C, 6 months; -20C, 1 month。-80C 储

3、存时，请在 6 个内使，-20C 储存时，请在 1 个内使。BIOLOGICAL ACTIVITY物活性 Naringin烷酮糖苷，有很多药理作，例如抗氧化活性，降脂，抗癌，抑制细胞素P450酶。IC & Target Human Endogenous Metabolite(批量添加)体外研究Naringin suppresses NF- B signaling pathway activation. Naringenin inhibits high glucose-induced proliferation,inflammatory reaction and oxidative stress

4、 injury in HBZY-1 cells1. Naringin inhibits AGS cancer cell proliferation in adose- and time-dependent manner. Phosphorylation of PI3K and its activated downstream targets p-Akt and p-mTORare significantly decreased at 2 mM in Naringin-treated AGS cells. Naringin induces autophagic cell death in AGS

5、cells. Naringin activated the autophagy related protein in AGS cells2. Naringin protects PC12 cells from 3-NPPage 1 of 2 www.MedChemEneurotoxicity. The lactate dehydrogenase release is decreased upon naringin treatment in 3-NP-induced PC12 cells.Naringin treatment enhances the antioxidant defense by

6、 increasing the activities of enzymatic antioxidants and thelevel of reduced glutathione3.体内研究 Treatment with naringin significantly alleviates renal injury in diabetic rats and increases diabetic rats body weightsignificantly. Administration of naringin effectively alleviates the collagen depositio

7、n and renal interstitial fibrosis indiabetic rats. Treatment with naringin could result in decreased levels of ROS and MDA and increased activities ofSOD and GSH-Px1. Oral administration of naringin significantly improves the learning and memory abilities. Naringinsignificantly enhances insulin sign

8、aling pathway3.PROTOCOLCell Assay 1 HBZY-1 cells are plated into 96-well plates and pretreated with various concentrations(1, 5, 10, 25, 50, 100 M) ofnaringin for 2 h. Then cells are treated with 30 mM glucose for 24 h. The control group is added sterile normal salinein the same volume. After treatm

9、ent, all the wells are incubated with 20 L of 5 mg/ml MTT for 4 h at 37C.Subsequently, 100 L of DMSO are used to dissolve the formed formazan crystals after removal of the supernatant.The result is recorded at 490 nm on a microplate reader1.MCE has not independently confirmed the accuracy of these m

10、ethods. They are for reference only.Animal Rats: The rats are randomly divided into six groups: control, naringin (80 mg/kg), STZ, STZ+naringin (20 mg/kg),Administration 14 STZ+naringin (40 mg/kg), STZ+naringin(80 mg/kg). The rats in the STZ and STZ+naringin groups areintraperitoneally injected with

11、 STZ (65 mg/kg). The control and naringin groups are intraperitoneally injected with 0.1M citrate buffer of same volume. After injection of STZ for 3 and 5 days, blood glucose levels are measured by tailvein puncture blood sampling1.Mice: Sixty 4-week-old male mice are randomized into four groups an

12、d fed for 20 weeks with either control diet orhigh-fat diet chow. Mice are dosed with 100 mg/kg of naringin daily. Mice body weight and food intake are weeklymeasured. Following behavioral assessment, animals are deeply anesthetized with isoflurane and sacrificed bydecapitation after fasting for at

13、least 5 h. Their plasma is collected for further analysis4.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Chen F, et al. Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction. PLoS One. 2

14、015 Nov30;10(11):e0143868.2. Raha S, et al. Naringin induces autophagy-mediated growth inhibition by downregulating the PI3K/Akt/mTOR cascade via activation of MAPK pathwaysin AGS cancer cells. Int J Oncol. 2015 Sep;47(3):1061-9.3. Kulasekaran G, et al. Neuroprotective efficacy of naringin on 3-nitr

15、opropionic acid-induced mitochondrial dysfunction through the modulation of Nrf2signaling pathway in PC12 cells. Mol Cell Biochem. 2015 Nov;409(1-2):199-211.4. Wang D, et al. Naringin Improves Neuronal Insulin Signaling, Brain Mitochondrial Function, and Cognitive Function in High-Fat Diet-Induced ObeseMice. Cell Mol Neurobiol. 2015 Oct;35(7):1061-71.McePdfHeig