常用凝胶参数

1、常用凝胶G技术参数1.葡聚糖凝胶内某些技术数据种类干颗粒直径（小）分子量分级范围床体积毫升/克干分子筛得水值溶胀最少平衡时间（h）柱头压力（kPa）（2.5cm直径柱）肽及球形蛋白质葡聚糖（线性分子）室温沸水浴SephadexG-1040120700700231.00.131SephadexG-1040120150015002.53.51.53.531SephadexG-25粗级100300(=5100目)中级50150(-100200目)1,0005,0001005,000461.50.262细级20800(弋200400目)超细1040SephadexG-50粗级100200中级50150

2、1,500500细级208030,00010,0009115.00.362超细1040SephadexG-75401203,0001,000超细104070,000950,00012157.50.52433.9215.86SephadexG-100401204,0001,000超细10401,500,00050,000152010.01.04852.359.41SephadexG-150401205,0001,0002030超细1040400,000150,000182215.01.57250.883.53SephadexG-200401205000100030401040800,000200

3、,000202520.02.07250.391.572.聚丙烯酰胺凝胶CD技术数据型号排阻内下限（分子量）分级分离范围（分子量）膨胀后内床体积（ml/g干凝胶）膨胀所需最少时间（室温,h）Bio-gel-P-21,6002002,0003.824Bio-gel-P-43,6005004,0005.824Bio-gel-P-64,6001,0005,0008.824Bio-gel-P-1010,0005,00017,00012.424Bio-gel-P-3030,00020,00050,00014.91012Bio-gel-P-6060,00030,00070,00019.01012Bio-ge

4、l-P-100100,00040,000100,00019.024Bio-gel-P-150150,00050,000150,00024.024Bio-gel-P-200200,00080,000200,00034.048Bio-gel-P-300300,000100400,00040.0483.琼脂糖凝胶内技术数据型号琼脂糖含量%（W/vy排阻内下限（分子量）分级分离内范围（分子量）生产厂家Sepharose4B40.3X1063X106PharmaciaSepharose2B22X10625X106Sagavac10102.5X1051X1042.5X105SeravacSagavac88

5、7X1052.5X1047X105Sagavac662X1065X10-2X106Sagavac4415X1062X10515X106Sagavac22150X1065X10515X107Bio-gelA-0.5M100.5X1061X104-0.5X106Bio-gelA-1.5M81.5X106密子第位1端CUALeuCCAProCAAGlnCGAArgACUGLeuCCGProCAGGlnCGCArgGAUUIleACUThrAAUAsnAGUSerAUCIleACCThrAACAsnAGCSerqAAUAIleACAThrAAALysAGAArgAAUGMetACGThrAAGLysA

6、GCArgGUUValGCUAlaGAUAspGGUGly口GUCValGCCAlaGACAspGGCGlyGGUAValGCAAlaGAAGluGGAGlyAGUGValGCGAlaGAGGluGGGGly目九、常用酸碱技术参数1.常见内市售酸碱内浓度溶质分子式分子量mol/Lg/L重量(%比重配制mol/L溶液内加入量(ml/L)冰乙酸CHCOOH60.0517.40104599.51.05057.5乙酸60.056.27376361.04559.5甲酸HCOOH46.0223.40080901.200z工2.7盐酸HCl36.5011.604124361.18036.22.9010510

7、1.050344.8硝酸HNO63.0215.991008711.42062.514.90938671.40067.113.30f337611.37075.2高氯酸HclOa100.5011.651172701.67085.89.20923601.540108.7磷酸HPO80.0018.101445851.70055.2硫酸H2PQ98.1018.00776961.84055.6多氧化钱NHOH35.0014.80:?51280.89857.6多氧化钾KOH56.1013.507节7501.52074.11.9410910II1.090515.5多氧化钠NaOH40.0019.10，63501.53052.42.75111101.110363.42.各种浓度CD酸碱贮存液CD近似pH值溶质1N30.1Na0.01Na0.001Na乙酸0.402.903.403.90盐酸0.101.072.023.01硫酸0