缓冲液配制方法

1、.常用缓冲溶液的配制方法1 甘氨酸一盐酸缓冲液(0.05mol/L)x ml 0.2mol/L 甘氨酸+ y ml 0.2mol/L HCl，再加水稀释至 200mlpHx/mly/mlpHx/mly/ml2.25044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸Mr = 75.07，0.2mol/L甘氨酸溶液含15.01g/L2 甘氨酸一氢氧化钠缓冲液(0.05mol/L)x ml 0.2mol/L 甘氨酸+ y ml 0.2mol/L NaOH，加水稀释至 200mlpHx/ml1y/mlpHx/mly/

2、ml8.6504.09.6508.8506.09.85027.29.0508.810.05032.09.25012.010.45038.69.45016.810.65045.5甘氨酸Mr = 75.07 , 0.2mol/L甘氨酸溶液为15.01g/L3.邻苯二甲酸一盐酸缓冲液(0.05mol/L, 20C)x ml 0.2mol/L邻苯二甲酸氢钾+ y ml 0.2mol/L HCl，再加水稀释到 20mlpHx/mly/mlpHx/mly/ml2.254.6703.251.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.

3、052.032邻苯二甲酸氢钾 Mr = 204.23，0.2mol/L邻苯二甲酸氢钾溶液含 40.85g/L4.柠檬酸一氢氧化钠一盐酸缓冲液pHNa +浓度/mol/L柠檬酸/gC6H8O7- H2O氢氧化钠/gNaOH(97%)盐酸/mlHCl(浓)最终体积/L*2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610使用时可以每升中加入 1g酚，若最后pH值有变化，再用少量 50%氢氧化钠溶液或浓盐酸调节，置

4、冰箱保存5.磷酸氢二钠一柠檬酸缓冲液pH0.2ol/LNa2HPO4/ml0.1mol/L柠檬酸/mlpH0.2mol/LNa2HPO4/ml0.1mol/L柠檬酸/ml2.20.4019.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.5

5、54.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.仃1.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.553Na2HPO4 Mr = 141.98 , 0.2mol/L 溶液为 28.40g/L,Na2HPO4 2H2O Mr =仃8.05 , 0.2mol/L 溶液为 35.61g/LC6H8O7 H2O Mr = 210.14 , 0.1mol/L 溶液为 21.01g/L6 柠檬酸一柠檬酸钠缓冲液（O.1mo

6、l/L）pH0.1mol/L柠檬酸/ml0.1mol/L柠檬酸钠/mlpH0.1mol/L柠檬酸/ml0.1mol/L柠檬酸钠/ml3.018.61.45.08.211.83.217.2_2.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39.76.61.418.64.89.210.8柠檬酸 C6H8O7 H2O, Mr = 210.14 , 0.1mol/L 溶液为

7、21.01g/L柠檬酸钠 Na3C6H5O7 2H2O, Mr = 294.12 , 0.1mol/L 溶液为 29.41g/L7.乙酸一乙酸钠缓冲液(0.2mol/L, 18C)pH0.2mol/LNaAc/ml0.2mol/LHAc/mlpH0.2mol/LNaAc/ml0.2mol/LHAc/ml3.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.904.64.905.105.89.400.608.邻苯二甲酸氢钾一氢氧化钠缓冲

8、液50ml 0.1mol/L邻苯二甲酸氢钾+ x ml 0.1mol/L氢氧化钠，加水稀释到100mlpHx/mlpHx/mlpHx/ml4.11.34.816.55.536.64.23.04.919.45.638.84.34.75.022.65.740.64.46.65.125.55.842.34.58.75.228.85.943.74.611.15.331.64.713.65.434.1邻苯二甲酸氢钾 Mr = 204.23 , 0.1mol/L溶液为20.42g/L9.磷酸盐缓冲液磷酸氢二钠一磷酸二氢钠缓冲液(0.2mol/L )pH0.2mol/LNa2HPO4/ml0.2mol/LN

9、aH2PO4/mlpH0.2mol/LNa2HPO4/ml0.2mol/LNaH 2PO4/ml5.88.092.07.061.039.05.910.090.07.167.033.06.012.387.77.272.028.06.115.085.07.377.023.06.218.581.57.481.019.06.322.577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.051.08.094.75.36.955.045.0Na2

10、HPO4 H2O, Mr = 178.05 , 0.2mol/L 溶液为 35.61g/L#Na2HPO4S2H2O, Mr = 358.22 , 0.2mol/L 溶液为 71.64g/LNaH2PO4 H2O, Mr = 138.01 , 0.2mol/L 溶液为 27.6g/LNaH2PO4 2H2O, Mr = 156.03 , 0.2mol/L 溶液为 31.21g/L(2)磷酸氢二钠一磷酸二氢钾缓冲液(1/15mol/L)pH1/15mol/LNa2HPO4/ml1/15mol/LKH 2PO4/mlpH1/15mol/LNa2HPO4/ml1/15mol/LKH 2PO4/ml4

11、.920.109.907.177.003.005.290.509.507.388.002.005.911.009.007.739.001.006.242.008.008.049.500.506.473.007.008.349.750.256.644.006.008.679.900.106.815.005.008.1810.0006.986.004.00Na2HPO4 2H2O, Mr =仃8.05 , 1/15mol/L 溶液为 11.876g/LKH2PO4, Mr = 136.09 , 1/15mol/L 溶液为 9.078g/L10 .磷酸氢二钠一氢氧化钠缓冲液50ml 0.05mol/

12、L 磷酸氢二钠+ x ml 0.1mol/L NaOH，加水稀释至 100mlpHx/mlpHx/mlpHx/ml10.93.311.37.611.716.211.04.111.49.111.819.411.15.111.511.111.923.011.26.311.613.512.026.9Na2HPO4 2H2O, Mr =仃8.05 , 0.05mol/L 溶液为 8.90g/L11.磷酸二氢钾氢氧化钠缓冲液(0.05mol/L, 20C)x ml 0.2mol/L KH 2PO4+ y ml 0.2mol/L NaOH，加水稀释至 20mlpHx/mly/mlpHx/mly/ml5.8

13、50.3727.052.9636.050.5707.253.5006.250.8607.453.9506.451.2607.654.2806.651.7807.854.5206.852.3658.054.68012 .硼砂一盐酸缓冲液(0.05mol/L硼酸根)50ml 0.025mol/L 硼砂+ x ml 0.1mol/L 盐酸，加水稀释至 100mlpHx/mlpHx/mlpHx/ml8.0020.58.416.68.89.48.1019.78.515.28.97.18.2018.88.613.59.04.68.3017.78.711.69.12.0硼砂 Na2BQ740HQ , Mr

14、= 381.43 , 0.025mol/L 溶液为 9.53g/L13 .巴比妥钠一盐酸缓冲液（18 C）pH0.04mol/L巴比妥钠溶液/ml0.2mol/L盐酸/mlpH0.04mol/L巴比妥钠溶液/ml0.2mol/L盐酸/ml6.810018.48.41005.217.010017.88.61003.827.210016.78.81002.527.410015.39.01001.657.610013.49.21001.137.810011.479.41000.708.01009.399.61000.358.21007.21巴比妥钠盐 Mr = 206.18 , 0.04mol/L

15、溶液为 8.25g/L14. Tris盐酸缓冲液(0.05mol/L, 25C)50ml 0.1mol/L三羟甲基氨基甲烷（Tris）溶液与x ml 0.1mol/L 盐酸混匀后，加水稀释至100mlpHx/mlpHx/ml7.1045.78.1026.27.2044.78.2022.97.3043.48.3019.97.4042.08.4017.27.5040.38.5014.77.6038.58.6012.47.7036.68.7010.37.8034.58.808.57.9032.08.907.08.0029.29.005.7注：三羟甲基氨基甲烷（Tris）Mr = 121.14，0.1

16、mol/L溶液为12.114g/L。Tris溶液可从空气中吸收二氧化碳，保存时注意密封15 .硼酸一硼砂缓冲液（0.2mol/L硼酸根）pH0.05mol/L硼砂/ml0.2mol/L硼酸/mlpH0.05mol/L硼砂/ml0.2mol/L硼酸/ml7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼酸 HaB03, Mr = 61.84，0.2mol/L 溶液为 12.37g/L16.硼砂一氢氧化钠缓冲液（0.05mol/L硼酸根）x ml 0.05mol/L 硼砂+ y ml 0.2mol/L

17、NaOH，加水稀释至 200mlpHx/mly/mlpH1x/mly/ml9.350&09.85034.09.45011.010.05043.09.65023.010.15046.0硼砂 Na2BQ7 40HQ , Mr = 381.43 , 0.05mol/L 溶液为 19.07g/L仃.碳酸钠一碳酸氢钠缓冲液（0.1mol/L）Ca"、Mg2+存在时不得使用pH0.1mol/L 碳酸钠 /ml0.1mol/L碳酸氢钠/ml20 C37 C9.168.77199.409.12289.519.40379.789.50469.909.725510.149.906410.2810

18、.087310.5310.288210.8310.5791无水碳酸钠 Mr = 105.99 , 0.1mol/L 溶液为 10.60g/L碳酸氢钠 Mr = 84.01 , 0.1mol/L 溶液为 8.40g/L18.碳酸氢钠一氢氧化钠缓冲液（0.025mol/L碳酸氢钠）50ml 0.05mol/L 碳酸氢钠+ x ml 0.1mol/L NaOH，加水稀释至 100mlpHx/mlpHx/mlpHx/ml9.65.010.112.210.619.1硼砂Na2B4O7S0HQ , Mr = 381.43 , 0.05mol/L溶液（=0.2 mol/L硼酸根）为19.07g/L，硼砂易失去结晶水，必须密闭保存99.76.210.213.810.720.29.87.610.315.210.821.29.99.110.416.510.922.010.010.710.517.811.022.7碳酸氢钠 Mr = 84.01 ; 0.05mol/L 溶液为 4.20g/L佃.氯化钾一氢氧化钠缓冲液25ml 0.2mol/L KCl + x ml 0.2mol/L NaOH，加水稀释至 100mlpHx