五加双参片对辐射和化学物质损伤细胞的影响

1、五加双参片对辐射和化学物质损伤细胞的影响         07-08-22 11:00:00     编辑：studa20作者：肖引，陈崇尚，华君，白娟，马勇智【关键词】  五加双参片；药理学；辐射损伤；化学损伤    【Abstract】 AIM: To study the protective effects of WUJIASHUANGSHEN tablets (WJSST)  on cells against radi

2、ation damage and chemical injury. METHODS:  The radiation damage models in rats were induced by 60Co as the radiant substance. We counted the numbers of  the peripheral blood leucocytes,  medullary cells and   the micronuleated cells and measured the content of DNA in thymoc

3、ytes. The models of hypocytosis were  induced by cyclophosphamide and the numbers of the leucocytes and thrombocytes were counted. RESULTS: When WJSST were administered after radiation damage by 60Co, the numbers of the leucocytes and medullary cells were  obviously increased as compared w

4、ith blank group and leucogen control group (P<0.01). The percentage of the micronuleated cells in the  WJSST  groups was markedly lower than that in  model group (P<0.01). As to  the content of DNA in the thymocytes, the  WJSST  groups were significantly higher th

5、an that in the model  group (P<0.01). When WJSST were administered after chemical injury by cyclophosphamide, the numbers of the leucocytes and thrombocytes were markedly increased as compared with  those of the blank  group and control groups (P<0.01). CONCLUSION: WJSST have th

6、e protective effects against the radiation damage and chemical injury such as the increasing  peripheral blood leucocytes  and medullary cells, the decreasing  the number of micronucleated cells, the increasing the content of DNA in thrombocytes.    【Keywords】  WUJ

7、IASHUANGSHEN tablet; pharmacology; radiation injuries; chemical injuries    【摘要】  目的： 研究五加双参片对60Co辐射损伤细胞的影响. 方法： 采用健康小鼠作为实验对象，五加双参片灌胃给药，使用60Co作为辐射物制备辐射损伤模型，采血记数外周血白细胞及骨髓造血细胞数、骨髓微核细胞数并测定胸腺DNA含量；环磷酰胺（CY）制备低血细胞模型，采血测定白细胞和血小板总数；观察五加双参片对辐射损伤和化学物质损伤的保护作用. 结果： 对保护辐射损伤外周血白细胞与骨髓造血细胞，五加双参片

8、能使损伤细胞得到恢复，加速其增殖与激活，与空白组比较（P0.01），优于利血生对照组（P0.01）；五加双参片组骨髓微核细胞千分率低于模型对照组（P0.01）；同时五加双参片组辐射小鼠胸腺DNA含量明显高于模型对照组（P0.01）；五加双参片还能防治CY所致的白细胞和血小板减少，与空白组比较P0.01，优于对照组（P0.05）.  结论： 五加双参片对辐射损伤细胞与骨髓造血细胞和对防治CY所致的白细胞和血小板减少症均有保护作用. 五加双参片能保护骨髓细胞，减轻60Co辐射对骨髓细胞的损害；对胸腺细胞DNA有保护作用.     【关键词】 五加双参片；药理

9、学；辐射损伤；化学损伤      五加双参片为临床应用多年的纯中药制剂，由刺五加、当归、玄参、党参等多味中药精制而成. 具有扶正固本、益气补血之功效. 主要用于肿瘤患者放化疗后出现的气血两虚证及白细胞减少者的辅助治疗1. 研究表明，本品能促进造血干细胞的增殖和分化；对辐射损伤的脾组织结构恢复有明显的促进作用；对环磷酰胺（CY）与60Co辐射引起的外周血细胞减少有明显升高作用. 为进一步深入探讨五加双参片对放疗患者的辅助治疗作用机制，我们采用健康小鼠作为实验对象，使用60Co作为辐射物制备辐射损伤模型，CY作为化学物质制备骨髓抑制和低血细胞模型，

10、观察了五加双参片对辐射损伤和化学物质损伤的保护作用.    1材料和方法    1.1材料五加双参片 (西安中药厂生产，030901，每片相当生药材0.69 g)；芪枣冲剂对照品厦门中药厂，批号（86）20749. 昆明种小鼠（第四军医大学实验动物中心提供），体质量1822 g.    1.2方法    DNA的含量=测定液中DNA的含量(g)×提取液总量(mL)/胸腺质量(g) ×106×测定所用提取液量(mL)×100%    统计学处理：  实验数据用x±s表示，进行单因素方差分析及Dunnett' t检验，运用SPSS11.5统计软件包处理数据，检验水准=0.05，以P<0.05为有显著性差异.    2结果    2.1五加双参片对60Co辐射小鼠外周血细胞和骨髓造血细胞的影响五加双参片对辐射损伤细胞与骨髓造血细胞有一定的保护作用，能使损伤脾细胞得到恢复，