拉曼光谱应用介绍.

1、拉曼光谱的应用ThermoThermo FisherThermoThermo Fisher拉曼应用characteristic Raman frequenciescomposition of material 物质的组成同分异性体， 同分异构体 相同元索不同拉曼频率的确认结构mchanges in frequency of Raman peak 拉曼峰位的变 化 width of Raman peak 拉曼峰宽stress/strain State张力f应力高分子纤维拉伸 薄膜退火温度 半导体器件mquality of crystal 晶体质量晶体化程度aintensity of Raman

2、peak 拉曼峰强度amount of material 物质总量薄膜厚度！拉曼光谱除了鉴别物质种类，主要测量分子振动频率，定 量了解分子间作用力和分子内作用力情况，并推断分子的 对称性，几何形状，分子中原子排列。拉曼应用无机材料：很多无机材料是中心对称的振动，红外光谱不敏感。而拉 曼光谱只有很明显的优势。高分子材料：单体结构测定，结品度，It架分犷解析，组分分析，裂解原位研究，纤维拉伸应力应变，催化材料：表而吸附研究（排除基体扰，原位条件卜.（岛温、高1K）催化过程动力学研究，分犷筛席架结构分析，物相变 化纳米材料：纳米尺度的表而效应，体积效应，邑犷尺寸效应以及物理 特性（如碳管的半导体特性

3、与金属效应）都会在拉处光谱上 得到体现生命科学：蛋白质、核酸、磷脂在疋常生理条件下的结构、构相。药物与细胞作用，Thermo< 11 n t I r I cThermo FisherSCIENTIFIC拉曼应用司法鉴定，理化分析、汕漆.橡胶.纤维.冷品，爆炸物.文检，痕迹检验 考古/艺术品：分析颜料、锈蚀氧化物、青铜器、玉器、陶瓷、粘七、般品 地质/珠宝：珠宋鉴定，矿物分析，包裹体成分分析，矿物随温度压力和变 药学： 晶空检验、原材料辅料分析鉴别、药片成分分布，候选新约筛选，假药鉴别，杂质分析，注射液成分分析，溶出度分析半导体：应力/应变，表而损伤，掺杂等环境科学：温空气体与燃烧气体与建

4、筑物反应研究，人气污染，重金属污染碳材料：纳米碳管，石墨，碳纤维，DLC膜，C60y 卩其实几乎所有的红外用户都有购买拉曼光谱仪的可能性！ThermoThermo Fisher应用市场分析ThermoThermo FisherThermoThermo Fisherfblymers1%Tobacco1%Gems-1%AutomotrvcSefncocxiuctor 2%、刃Art and Archaeotogy Aberglass 2% "I 1%S»eel1%ThermoThermo FisherContract Research 1%Agricultural Chcmic

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6、11 N T I r I cThermo FisherSCIENTIFIC司法科学文检不同复印机墨的拉 曼光谱比对ThermoThermo Fisher司法科学文检>»« BUX« W «««HP laserjet 3020不同激光打印机墨 的拉曼光谱比对 ne1 Konica714Laserjet 6LThermo» < 11 N T I r I cThermo» < 11 N T I r I cThermo» < 11 N T I r I cThermo FisherSCIEN

7、TIFICThermo» < 11 N T I r I c司法科学理化检验Thermo» < 11 N T I r I cThermo» < 11 N T I r I c汕漆漆片拉 曼光谱Thermo» < 11 N T I r I cThermo FisherSCIENTIFICThermo* c 11 n r i r i c司法科学文检不同纤维拉曼光谱780nm激发ThermoC 11 M T I r I cThermo FisherSCIENTIFIC司法科学文检SirMtelMilMRTIkAinfrtiliielhePe

8、r pohllRirMnMknt 780 nm laser 10 sec exposure time 2 exposuresThermo FisherSCIENTIFICThermo* c 12 n r i r i c司法科学理化分析Ihenno FisherThermoC 11 N V I v13Ihenno FisherThermoC 11 N V I v#TNT Specimen 785 nm laser 10 sec on ds collectionThermotk < 11 n t i r i cMThermo Fisher SCIENTIFICIhenno FisherThe

9、rmoC 11 N V I v#司法科学-理化Ihenno FisherThermoC 11 N V I v#Ihenno FisherThermoC 11 N V I v#言 SU3Sse£Ihenno FisherThermoC 11 N V I v14司法科学卖点自动准直光路，自动曝光，自动校正，自动扌II除荧光。真正的自动化是该领域的最大卖点！强调可以分析小于的痕量样品以及样品区域，可分 析现场残留的微最物。强人的数据库，深入拓展用户的应用！暗场照明低波数50cm有利于漆片，爆炸物等无机显微镜H镜，排掉JY的LabRam-HR强大的售后服务！合适的价格可以推广到局一级的单位！

10、ThermoThermo FishertcitNTiric13SCIENTIFIC文物考古书画考古：(1)印泥成分鉴定:a.b.真品,c贋品1,d廣品2Thermo FisherThermo14文物考古书画（2）拉曼光谱分析:ab真品，c. 廣品1,d. 贋品2Thermo文物考古青铜器优点 1.青铜锈蚀物的原位分析2.微量取样分析3多层锈蚀物的显微形貌观察及结构分析国内外有很多拉曼仪器研究人员曾涉及青铜及梵锈蚀物方面的研究, 美国密西根州立人学的Lowell I.McCan n教授6曾对中国东汉的 一件青铜摇钱树的腐蚀情况作了非常系统的研究，并报告了几种常 见青铜锈蚀产物的拉曼光谱图。国内云

11、南楚雄师范学院的上怡林等Thermo FisherThermo17Thermo FisherThermo#文物考古青铜器见青铜锈蚀物一览表颜色锈蚀矿物绿色碱式碳酸铜(孔雀石)CuCO3-Cu(OH)2碱式氯化铜(氯铜矿或副氯铜矿)CuCI2-3Cu(OH)2蓝色碱式碳酸铜(蓝铜矿)2CuCO3-Cu(OH)2碱式硫酸铜2CuSO4-3Cu(OH)2-H2O红色氧化亚铜(赤铜矿)Cu2O黑色氣化铜CuO白色碳酸铅PbCO3碱式碳酸铅2PbCO3-Pb(OH)2锡石SnO2其它Cu2SCuSThermoThermo Fisher citnr ir ic17SCIENTIFIC文物考古-腐蚀兀代断剑

12、I：的锈蚀物Thermo文物考古-颜料WE红色赤01矿Fe2O3wnPb3O4朱砂 HgStT 1 (red earth)Fe2O3貼轮規L"；枚 »加色幺钢矿CuCI2-3Cu(OH)2IWI*b|i«W4i却 CuCO3Cu(OH)2»故色石育CuCO3Cu(OH)2汉般与汉紫毎麻离时航«91色am緞01矿KU力解石骨白铅白黄色维MPbO红色：朱砂在激尤卜腸于变九不艳定. 恿色 «饮矿庄就）t卜44产生变化. n&i wnsit拉蚁侑'；很删.蓟色，人色色混杂A.匕 “不总疋“董朮勺対效应.Thermo* c 11

13、 n v I r I cThermo Fisher文物考古-颜料Thermo22Thermo#文物考古技术推广自动准直光路，自动曝光，自动校匚 自动扌II除荧光。水平光路！光纤探头！强人数据库!显微镜暗场照明!低波数50cm-1 Yf利于氧化物分析至少80级的激光功率调节,显微镜冃镜，排掉J丫的LabRam-HR强人的售后服务!Thermo FisherThermo# Diam ond sp3bonded carbon Graphite sp2bonded carbon碳材料拉曼光谱 Sheet of aromatic rings Fullere nes Hollow carb on sphe

14、res Diamond-like Carbon (DLC) coatings Amorphous Carbon Properties similar to Diamond Sometimes hydroge nated Different forms sometimes called polymorphs More accurately allotropesThermoThermo Fisher碳材料Graphe ne Single layers from Graphite structure Graphene nan oribb oris (GNR) roll up to form nano

15、 tubesRoll-upSWNT SW-CNT MW-CNT Carbon nano wallsgraphene sheet Walled graphene structuresThermo FisherThermo24碳材料 Spectra are well characterized by RamanThermo< 11 n v I r I cThermo< 11 n v I r I cThermoThermo< 11 n v I r I c碳材料-RBM波段（呼吸模）表征单壁碳管管径间接农征于性与电特性Thermo< 11 n v I r I c对激发波长很敏感

16、Thermo FisherSCIENTIFICThermo< 11 n v I r I c碳材料 G Bands (Tangential or Graphite bands) Centered around 1580 cm*1 Represent C-C stretching mode Good for determining electrical property (metallic or semic on ducting) Broadened G-band indicates metallic碳材料 D bands (Diamond or Defect bands) Centered

17、 around 1335 cm" Indicate defects in SW-CNTs碳材料拉曼光谱 Rama n can be used to compare and con trast nano tubes for the purposes of fine-tuning a synthesis processThermo* c 11 n v i r i cThermo* c 11 n v i r i c two carbon peaks graphitic "G” disordered carb on MDn correlati ons D/G area/inte n

18、sity ratioThermoThermo Fisher otNf ir IC29SCIENTIFIC碳材料DLC模拉曼光谱correlates with wear resistance G band area and intensitycorrelates with thicknessG band position correlates with wear resistance correlates with hydrogenThermo* c 11 n v i r i c碳材料1M0 2XC IQ ®Aj1 .01003 2000 3000 4000 6000Afefvenim

19、ber (cm*)300nHG. 3. R>m«in scattering %pc:tra taken from difT;rcnt regions of an individual 8 pm lonj TGC M the sharp tip (a); 2.5 (b); 5 (c); and 7.5 “m (d) away fron the tip.Large-sized tubular graphite cones with nano tube tips.APPLIED PHYSICS LETTERS 87,163102 2005Thermo Fisher32Thermo F

20、isher#ThermoThermo F isher碳材料卖点自动准直光路，口动曝光，自动校E, 口动扌II除荧光。低波数50cnvW利于呼吸模的测量至少80级的激光功率调节，保证了碳管不会在激光热效 应下发生应力或者相变！633n m激发是首选！Thermo Fisher#Thermo Fisher#ThermoThermo Fisher#约学晶形鉴别晶形鉴别5-Methyl-2-(2-nitrophe nyl)ami no -3-thiophenecarbonjtrilealso known as uROYn *Thermo Fisher34Thermo Fisher#Yu. L.: St

21、強hen&on, G. A: Milcheli. C. A.: BunnL C. A.: Snow匕 S, V：Bowvg J, J.: Bcrchardl. T, B.: Slowell, J G: Bym. S R. J. Am. Own. Soc 2000, 122, 585ThermoThermo Fisher33SCIENTIFIC药学晶形鉴别Thermo Fisher#Thermo Fisher#ThermoThermo Fisher#药学晶形鉴别约学低波数信息 Spectrum of Amino Acid - Cystine Low frequency bands in

22、Raman Shift Raman analytical signal occurs near wavelength of the laser where easily sensed by the detector Far IR equipment not required药学卖点自动准直光路，口动曝光，口动校止，自动抑除荧光。性价比很高的Smart DXR Variable Dynamic Point Sampling 快速动态取样扫描Thermo FisherSCIENTIFICThermo» c 11 n v i r i c39Thermo FisherSCIENTIFICTh

23、ermo» c 11 n v i r i c#微孔板转盘式样吊附件可分析液体注射液低波数50cm-1 Yf利于药物的低波数测量解偏激光器|The Well-Plate AutosamplerThermo FisherSCIENTIFICThermo» c 11 n v i r i c#Solar PanelsThermos c 11 n r i r i cThermo Fisher38SCIENTIFIC太阳能材料 Crystalline Silicon High efficiency in IR only Expe nsive Ages quickly Amorphous

24、 Silicon Lower efficiency (no IR) Shows little aging Inexpensive Tandem cells of Micro-crystalline and amorphous Silicon High efficiency(iDeluding IR) Shows no agingEcono micalThermo FisherSCIENTIFICThermo» c 11 n v i r i c40太阳能材料 Morphology CharacterizationThermo FisherThermo41Crystalline Sili

25、con BandS4O5fiOSOO40O4eOMO42O«OThermoThermo FishericitMtieic40SCIENTIFICThermo FisherThermo#太阳能材料Thermo FisherThermo#Thermo FisherThermo#Po«ltton (rrtcrc<ne«te<5)Amorphous Silicon:BlueMicrocrystaline Silicon:RedThermo FisherThermo42工业客户卖点自动准直光路，自动曝光，自动校止，自动抑除荧光。件价比很高的Smart DXR,

26、DXR microscope强大的软件功能，TQ定量分析等强大的售后服务！Thermo FisherThermo43Thermo* c 11 n v I r I c42Thermo FisherSCIENTIFICThermo FisherThermo#掺杂对拉曼光谱影响Thermo FisherThermo#Thermo FisherThermo#2004006008001000Raman shift (cm-1)3 RuiTKtn xpoulra of (he cenunics v%iih difkrvnt Sb conlcnK.422=lii(K044NaC.52Li004)(Nb080

27、xTa020Sbx)O3 piezoelectric ceramicsSbO的键能(372 kJ/mol)要比NbO的键能(753 kJ/mol)低J. Am. Ceram. Soc., 91 72211-2216 (2008)Thermo FisherThermo44结晶度Raman Shift (cm*1)Thermo FisherSCIENTIFICThermoC 11 N T I r I cThermo FisherSCIENTIFICThermoC 11 N T I r I cRaman spectra of crystalline and amorphous polyethylen

28、e terephthalate films.ThermoC 11 N T I r I cThermo FisherSCIENTIFIC聚合物生长Thermo FisherSCIENTIFICThermoC 11 N T I r I cThermo FisherSCIENTIFICThermoC 11 N T I r I cJ. Am. Chem. Soc. 2008, 130 (24), 7702-7709Thermo FisherSCIENTIFICThermoC 11 N T I r I c环境科学：温室与燃烧物气体对建筑材料的腐蚀CO2, SOx与 NOx气体CaOHydrationpr

29、oc."STEP 1Atmosphetic AaCO attackC a(OH2 C aC O$2 5Te 1 (CakMe endfo* Ar»QO«wt. t>o9ymorptw»c forvn«)CaSO4.2H2O(Gypsum)MKl/OfCaSO4(AntwdntelCaNO,4HQ(Nitrcxuilcito)Thermo FisherThermo47FQiiro 6. OvotaII dwgradAtion proc:*a for calcium (And mAgn««itirn or t>ari

30、um) comrxMinciHbrtckAtmosphericCO. attackHydrationprocessNa2CO3.10H2O(Nttron)Na2SO4.2H2O* (Mirabiltte) *and/orNa:SO4(Then.dgNaNO,(NHratine>Thermo FisherThermo#Tl Figure 7. Overall degradation process for sodium compounds.环境科学温室与燃烧物气体对建筑材料的腐蚀研究Table 1 Raman characteristic bands &: strong, m: m

31、edium. w: weak v: very) of original materials and common degradation products arid pollutants affecting to stone rnatwials of Built HeritageCompoundFormulaWavcnumtxrs/cm 1GypournCaSOj 2HQ1132 tn, 1008 » 673 m( 619 n, 4M2 mz 413 inAnhidriteGaSO41160 w, 1130 mr 1112 w. 1017 vsf 675 m, 627 m, 60Q m“ 50) m, 417 mMirabiliteNa?SOj 10H2O1152 m, 1132 m, 1102vs, 645 m, 632 m,620 m, 466 w, 458 m.452 wThenarditeNa2SO41780 w, 1712 w, 1320 w. 1258 m, 1201 w188 w, 1155 w. 1136 w. 1105 w.995 vs, 626 m, 432 mCalciteGaLCh1436 wr 1085 vs, 712 m,2«l mAragoniteCaCCh1463 vw, 1085 vs, 705 rr, 205 m