兔胆与熊胆对缓解小鼠呼吸道过敏发炎反应之比较

1、兔膽與熊膽對緩解小鼠呼吸道過敏發炎反應之比較蕭宏柏1詹明修2施維真3謝長奇3, 41中興大學 生命科學系2中山醫學大學 醫學應用微生物研究所3東海大學 畜產與生物科技學系4中國醫藥大學 中藥資源學系（收件日期：98年6月30日；接受日期：98年8月25日）摘要 民間相信，陸棲哺乳類野生動物具有某些醫療功效，而增加其獵捕壓力，在保護保育類野生動物的前題下，某些藥方因此不能處方、調劑，開發類似醫療功效之中藥資源極具意義。熊膽於臨床應用上有治療急慢性肝炎、黃疸性肝炎、下痢、病毒性咽喉炎、急性扁桃腺炎等，由於兔膽藥理作用較接近熊膽，具有抗驚厥、抗疲勞與鎮咳的作用，對呼吸道過度反應有緩解的作用。本研究利

2、用呼吸道過度反應模式與細胞培養之體外試驗評估這兩種藥材的藥理作用，試驗動物經犧牲後結果顯示，在呼吸道過度反應中，熊膽樣品（500 mg/kg）與兔膽樣品高劑量（250 mg/kg）亦可顯著降低巨噬細胞含量，並且可顯著降低腫瘤壞死引因子（tumor necrosis factors-, TNF-a）含量。體外試驗結果亦顯示熊膽樣品與兔膽樣品均具有明顯抑制一氧化氮（nitric oxide, NO）產生，與降低誘導發炎激素TNF-a 的產生，TNF-a 會誘導呼吸道與肺臟等上皮第一型細胞間質吸附分子（Intercellular Adhesion Molecule-1, ICAM-1）與第一型血管細

3、胞吸附分子（vascular cell adhesion molecule-1, VCAM-1）等吸附性蛋白質的表現，進而影響發炎細胞浸潤於呼吸道中。本試驗指出，由熊膽與兔膽所調節的發炎細胞激素TNF-a 顯著性降低，或許能降低吸附性蛋白質的呈現，進而緩解了發炎細胞的浸潤，與呼吸道的過度反應。本試驗結果亦顯示兔膽與熊膽樣品具有相同之藥理作用。關鍵詞：呼吸道過度反應、抗發炎、熊膽、兔膽、野生動物保育The reduced effect of rabbit bile and bear bile in murine airway allergic inflammationHung-Bo Hsiao

4、1, Ming-Shiou Jan2, Wei-Chen Shih3 and Chang-Chi Hsieh3, 4*1 Department of Life Science, National Chung Hsing University.2 Graduate Institute of Applied Medical Microbiology, Chung Shan Medical University3 Department of Animal Science and Biotechnology, Tunghai University4 School of Chinese Medicine

5、 Resources, China Medical University（Date received: Jun. 30, 2009; Date accepted: Aug. 25, 2009）AbstractFolk to believe that the terrestrial mammals might be contained medical efficacy. Because of the hunting stress and land capacity, their survival faces the great problem. In the wild animal conser

6、vation issue, the resources of conserved animal in traditional Chinese medicine can not used to prescription. It is important to development of new Chinese medicine resources for medical treatment. The clinical applications of bear bile were used to treat acute and chronic hepatitis, jaundice-hepati

7、tis, diarrhea, acute viral pharyngolaryngitis, and acute tonsillitis. The pharmacological effect of rabbit bile was closely to bear bile including anticonvulsant, anti-fatigue and relieving cough, which can be used to reduce the airway hyperresponsiveness. In this study, we use rabbit bile to be the

8、 replacement drug of bear bile in immuno-modulated effects of airway hyperresponsiveness. In animal study, bear bile (500 mg/kg) and rabbit bile (250 mg/kg) can significantly reduces macrophage infiltration in lung, and significantly reduce the tumor necrosis factor- (TNF-). In vitro study show of b

9、ear bile and rabbit bile can significantly inhibits nitric oxide (NO), and reducing inflammatory cytokine (TNF-). TNF- can induce the intercellular adhesion molecules-1 (ICAM-1) and vascular cell adhesion molecules (VCAM-1) expression in epithelium cells of lung and respiratory airway, and further accumulated the inflammatory cells infiltration. Our results indicated lower TNF- presentation might down-regulated adhesion molecules expression to inhibit the inflammatory cells infiltration, and further reduced the hyperresponsiveness. The rabbit and bear bile in this e