商品粮食中还原糖和非还原糖含量的测定

1、GB 551385本标准适用于商品粮食中还原糖和非还原糖含量的测定。其中铁氰化钾法主要适用于小麦粉。1还原糖测定1.1铁氰化钾法2WO42H2O溶于100 ml水中；V1）。V0）。还原糖含量先按公式（1）计算出氧化样品液中“还原糖”所需0.1 N铁氰化钾溶液体积（V3），再根据此体积（ml）查表1，即可查得试样中含还原糖（以麦芽糖计算）的百分数。按公式（1）计算：（1）式中：V0滴定空白液消耗铁氰化钾溶液体积，ml；V1滴定样品液消耗铁氰化钾溶液体积，ml；V3氧化样品液中还原糖所需0.1 N铁氰化钾溶液体积，ml；N硫代硫酸钠溶液实际当量浓度。双试验结果允许差不超过0.05，求其平均数，即

2、为检验结果。检验结果取小数点后第二位。注：还原糖含量以麦芽糖计算。1.2费林氏液法a碱性酒石酸铜甲液：取硫酸铜结晶34.639 g，加适量水溶解，加硫酸0.5 ml，再加水至 500 ml，用精制石棉过滤；b碱性酒石酸铜乙液：取酒石酸钾钠173 g与氢氧化钠50 g，加适量水溶解，稀释至500 ml，用精制石棉过滤，贮存于具有橡皮塞的玻璃瓶内。表 10.1N K3/SFe(CN)6 ml还原糖0.100.200.300.400.500.600.700.800.901.001.101.201.301.401.501.601.701.801.902.002.102.202.302.402.502.

3、602.702.802.903.003.103.203.303.403.503.603.703.803.904.004.104.204.304.404.504.604.704.804.905.005.105.205.305.405.505.605.705.805.906.006.106.206.306.406.506.606.706.806.907.007.107.207.307.407.507.607.707.807.908.008.108.208.308.408.508.608.708.800.050.100.150.200.250.310.360.410.460.510.560.600.

4、650.710.760.800.850.900.961.011.061.111.161.211.261.301.351.401.451.511.561.611.661.711.761.821.881.952.012.072.132.182.252.312.372.442.512.572.642.702.762.822.882.953.023.083.153.223.283.343.413.473.533.603.673.733.793.853.923.984.064.124.184.254.314.384.454.514.584.654.724.784.854.924.995.055.125.

5、19相当于试样中还原糖重量的氧化亚铜重量按公式（2）计算：x（VV0）N71.54（2）式中：x相当于试样中还原糖重量的氧化亚铜的重量，mg；V试样消耗高锰酸钾标准溶液的体积，ml；V0试样空白消耗高锰酸钾标准溶液的体积，ml；N高锰酸钾标准溶液的当量浓度；71.541 N高锰酸钾标准溶液1 ml相当于氧化亚铜的毫克数。由所得的氧化亚铜重量，按附录A的表查出相当的还原糖（以葡萄糖计）的重量。还原糖干基含量按公式（3）计算：（3）式中：A由附录A表中查得的还原糖（以葡萄糖计）的重量，mg；W试样重量，g；M试样水分百分率，；注：煮沸时间必须控制在4 min内。可先取水50 ml加碱性酒石酸铜甲、

6、乙液各25 ml，调节好适当的火力后，再作样品。煮沸后的溶液如不呈蓝色，表示糖量过高，可减少试样重量，另作。2非还原糖测定2.1铁氰化钾法）。非还原糖含量先按公式（4）计算，求出氧化样品液中总“还原糖”所需0.1 N铁氰化钾溶液所需的体积（V4），然后减去单独氧化样品液中“还原糖”所需的铁氰化钾溶液体积（V3）公式（1），最后再根据V4V3的结果（ml）查表2，即可查得试样中含非还原糖（以蔗糖计）的百分数。（4）式中：滴定空白液消耗硫代硫酸钠溶液体积，ml；滴定样品液消耗硫代硫酸钠溶液体积，ml；V4氧化样品液中总还原糖所需0.1 N铁氰化钾溶液体积，ml；N硫代硫酸钠溶液当量浓度。双试验结果

7、允许差不超过0.05，求其平均数，即为测定结果。测定结果取小数点后第二位。注：非还原糖含量以蔗糖计算。表 20.1N K3Fe(CN)6/SUml非还原糖0.100.200.300.400.500.600.700.800.901.001.101.201.301.401.501.601.701.801.902.002.102.202.302.402.502.602.702.802.903.003.103.203.303.403.503.603.703.803.904.004.104.204.304.404.504.604.704.804.905.005.105.205.305.405.505.6

8、05.705.805.906.006.106.206.306.406.506.606.706.806.907.007.107.207.307.407.507.607.707.807.908.008.108.208.308.408.500.050.100.150.190.240.290.340.380.430.480.520.570.620.670.710.760.810.860.910.951.001.041.091.141.191.231.281.331.381.431.481.521.571.611.661.711.761.811.851.901.952.002.042.092.142.1

9、82.232.282.332.382.422.472.512.562.612.662.702.752.802.802.902.942.993.043.093.133.183.233.283.333.373.423.473.523.573.623.673.723.773.823.873.923.974.024.072.2费林氏液法非还原糖干基含量（以蔗糖计）按公式（5）计算：（5）式中：A转化后测得的还原糖（以葡萄糖计）重量，mg；V转化后稀释至100 ml，用于测定还原糖的体积，ml；W原测定还原糖时试样重量，g；0.95还原糖（以葡萄糖计）换算为蔗糖的因数；M试样水分百分率，。附录A相当于氧

10、化亚铜重量的葡萄糖、果糖、转化糖重量表（补充件）相同于氧化亚铜重量的葡萄糖、果糖、转化糖重量见下表：mg氧化亚铜葡萄糖果 糖转化糖11.312.413.514.615.816.918.019.120.321.422.523.624.825.927.028.129.330.431.532.633.834.936.037.238.339.440.541.742.843.945.046.247.348.449.550.751.852.954.055.256.357.458.559.760.861.963.064.265.366.467.668.769.870.972.173.274.375.476.

11、677.778.879.981.182.283.384.485.686.787.888.990.191.292.393.494.695.796.897.999.1100.2101.3102.5103.6104.7105.8107.0108.1109.2110.3111.5112.6113.7114.8116.0117.1118.2119.3120.5121.6122.7123.8125.0126.1127.2128.3129.5130.6131.7132.8134.0135.1136.2137.4138.5139.6140.7141.9143.0144.1145.2146.4147.5148.

12、6149.7150.9152.0153.1154.2155.4156.5157.6158.7159.9161.0162.1163.2164.4165.5166.6167.8168.9170.0171.1172.3173.4174.5175.6176.8177.9179.0180.1181.3182.4183.5184.5185.8180.9188.0189.1190.3191.4192.5193.6194.8195.9197.0198.1199.3200.4201.5202.7203.8204.9206.0207.2208.3209.4210.5211.7212.8213.9215.0216.

13、2217.3218.4219.5220.7221.8222.9224.0225.2226.3227.4228.5229.7230.8231.9233.1234.2235.3236.4237.6238.7239.8240.9242.1243.1244.3245.4246.6247.7248.8249.9251.1252.2253.3254.4255.6256.7257.8258.9260.1261.2262.3263.4264.6265.7266.8268.0269.1270.2271.3272.5273.6274.7275.8277.0278.1279.2280.3281.5282.6283.

14、7284.8286.0287.1288.2289.3290.5291.6292.7293.8295.0296.1297.2298.3299.5300.6301.7302.9304.0305.1306.2307.4308.5309.6310.7311.9313.0314.1315.2316.4317.5318.6319.7320.9322.0323.1324.2325.4326.5327.6328.7329.9331.0332.1333.3334.4335.5336.6337.8338.9340.0341.1342.3343.4344.5345.6346.8347.9349.0350.1351.

15、3352.4353.5354.6355.8356.9358.0359.1360.3361.4362.5363.6364.8365.9367.0368.2369.3370.4371.5372.7373.8374.9376.0377.2378.3379.4380.5381.7382.8383.9385.0386.2387.3388.4389.5390.7391.8392.9394.0395.2396.3397.4398.5399.7400.8401.9403.1404.2405.3406.4407.6408.7409.8410.9412.1413.2414.3415.4416.6417.7418.

16、8419.9421.1422.2423.3424.4425.6426.7427.8428.9430.1431.2432.3433.5434.6435.7436.8438.0439.1440.2441.3442.5443.6444.7445.8447.0448.1449.2450.3451.5452.6453.7454.8456.0457.1458.2459.3460.5461.6462.7463.8465.0466.1467.2468.4469.5470.6471.7472.9474.0475.1476.2477.4478.5479.6480.7481.9483.0484.1485.2486.

17、4487.5488.6489.74.65.15.66.06.57.07.58.08.58.99.49.910.410.911.411.912.312.813.313.814.314.815.315.716.216.717.217.718.218.719.219.720.120.621.121.622.122.623.123.624.124.625.125.626.126.527.027.528.028.529.029.530.030.531.031.532.032.533.033.534.034.535.035.536.036.537.037.538.038.539.039.540.040.5

18、41.041.542.042.543.043.544.044.545.045.546.046.547.047.548.048.549.049.550.050.651.151.652.152.653.153.654.154.655.155.656.156.757.257.758.258.759.259.760.260.761.361.862.362.863.363.864.364.965.465.966.466.967.468.068.569.069.570.070.571.171.672.172.673.173.774.274.775.275.776.376.877.377.878.378.9

19、79.479.981.481.081.582.082.583.183.684.184.685.285.786.286.787.387.888.388.989.489.990.491.091.592.092.693.193.694.294.795.295.796.396.897.397.998.498.999.5100.0100.5101.1101.6102.2102.7103.2103.8104.3104.8105.4105.9106.5107.0107.5108.1108.6109.2109.7110.2110.8111.3111.9112.4112.9113.5114.0114.6115.

20、1115.7116.2116.7117.3117.8118.4118.9119.5120.0120.6121.1121.7122.2122.7123.3123.8124.4124.9125.5126.0126.6127.1127.7128.2128.8129.3129.9130.4131.0131.6132.1132.7133.2133.8134.3134.9135.4136.0136.5137.1137.7138.2138.8139.3139.9140.4141.0141.6142.1142.7143.2143.8144.4144.9145.5146.0146.6147.2147.7148.

21、3148.8149.4150.0150.5151.1151.7152.2152.8153.4153.9154.5155.1155.6156.2156.8157.3157.9158.5159.0159.6160.2160.7161.3161.9162.5163.0163.6164.2164.7165.3165.9166.5167.0167.6168.2168.8169.3169.9170.5171.1171.6172.2172.8173.4173.9174.5175.1175.7176.3176.8177.4178.0178.6179.2179.7180.3180.2181.5182.1182.

22、7183.2183.8184.4185.0185.6186.2186.8187.3187.9188.5189.1189.7190.3190.9191.5192.0192.6193.2193.8194.4195.0195.6196.2196.8197.4198.0198.5199.1199.7200.3200.9201.5202.1202.7203.3203.9204.5205.1205.7206.3206.9207.5208.1208.7209.3209.9210.5211.1211.7212.3212.9213.5214.1214.7251.3215.9216.5217.1217.8218.

23、4219.0219.6220.2220.8221.4222.0222.6223.3223.9224.5225.1225.7226.3227.0227.6228.2228.8229.5230.1230.7231.4232.0232.7233.3234.0234.7235.3236.1236.95.15.66.16.77.27.78.38.89.39.910.410.911.512.012.513.113.614.214.715.215.816.316.817.417.918.419.019.520.120.621.121.722.222.823.323.824.424.925.426.026.5

24、27.127.628.228.729.229.830.330.931.431.932.533.033.634.134.735.235.836.336.837.437.938.539.039.640.140.741.241.742.342.843.443.944.545.045.646.146.747.247.848.348.949.450.050.551.151.652.252.753.353.854.454.955.556.056.657.157.758.258.859.359.960.461.061.662.162.763.263.864.364.965.466.066.567.167.7

25、68.268.869.369.970.471.071.672.172.773.273.874.374.975.576.076.677.177.778.378.879.480.080.581.181.682.282.883.383.984.485.085.686.186.787.387.888.489.089.590.190.691.291.892.392.993.594.094.695.295.796.396.997.498.098.699.299.7100.3100.9101.4102.0102.6103.1103.7104.3104.8105.4106.0106.6107.1107.110

26、8.3108.8109.4110.0110.6111.1111.7112.3112.9113.4114.0114.6115.2115.7116.3116.9117.5118.0118.6119.2119.8120.3120.9121.5122.1122.6123.2123.8124.4125.0125.5126.1126.7127.3127.9128.4129.0129.6120.2130.8131.3131.9132.5133.1133.7134.2134.8135.4136.0136.6137.2137.7138.3138.9139.5140.1140.7141.3141.8142.414

27、3.0143.6144.2144.8145.4145.9146.5147.1147.7148.3148.9149.5150.1150.6151.2151.8152.4153.0153.6154.2154.8155.4156.0156.5157.1157.7158.3158.9159.5160.1160.7161.3161.9162.5163.1163.7164.3164.9165.4166.0166.6167.2167.8168.4169.0169.6170.2170.8171.4172.0172.6173.2173.8174.4175.0175.6176.2176.8177.4178.017

28、8.6179.2179.8180.4181.0181.6182.2182.8183.4184.0184.6185.2185.8186.4187.0187.6188.2188.8189.4190.1190.7191.3191.9192.5193.1193.7194.3194.9195.5196.1196.7197.3197.9198.5199.2199.8200.4201.0201.6202.2202.8203.4204.0204.7205.3205.9206.5207.1207.7208.3209.0209.6210.2210.8211.4212.0212.6213.3213.9214.521

29、5.1215.7216.3217.0217.6218.2218.8219.5220.1220.7221.3221.9222.6223.2223.8224.4225.1225.7226.3226.9227.6228.2228.8229.4230.1230.7231.3232.0232.6233.2233.9234.5235.1235.8236.4237.1237.7238.4239.0239.7240.3241.0241.6242.2242.9243.6244.3244.9245.6246.3247.0247.8248.5249.2250.0250.8251.6252.7253.75.25.76

30、.26.77.27.78.28.79.29.710.210.711.211.712.312.813.313.814.314.815.315.816.316.817.317.818.318.919.419.920.420.921.421.922.422.923.524.024.525.025.526.026.527.027.628.128.629.129.630.130.631.231.732.232.733.233.734.334.835.335.836.336.837.437.938.438.939.440.040.541.041.542.042.643.143.644.144.745.24

31、5.746.246.747.347.848.348.849.449.950.450.951.552.052.553.053.654.154.655.255.756.256.757.357.858.358.959.459.960.461.061.562.062.663.163.664.264.765.265.866.366.867.467.968.469.069.570.070.671.171.672.272.773.273.874.374.975.475.976.577.077.678.178.679.279.780.380.881.381.982.483.083.584.084.685.18

32、5.786.286.887.387.888.488.989.590.090.691.191.792.292.893.393.894.494.995.596.096.697.197.798.298.899.399.9100.4101.0101.5102.1102.6103.2103.7104.3104.8105.4106.0106.5107.1107.6108.2108.7109.3109.8110.4110.9111.5112.1112.6113.2113.7114.3114.9115.4116.0116.5117.1117.6118.2118.8119.3119.9120.4121.0121.6122.1122.7123.3123.8124.4124.9125.5126.1126.61