缓冲溶液配制表

1、常用缓冲溶液的配制方法1.甘氨酸-盐酸缓冲液(005mol/L)X亳升0.2 mol/L甘氨酸+Y亳升0.2 mol/L HCL再加水稀释至200亳升PHXYPHXY2.05044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.S3.65()5.0甘氨酸分子量=75.07, 0.2 mol/L甘氨酸溶液含15.01克/升。2.邻苯二甲酸-盐酸缓冲液(0.05 mol/L)X亳升0.2 moVL邻苯二甲酸氢钾+ 0.2 mol/L HC1,再加水稀释到20亳升pH(20°C)XYpH(20C)XY2.254.0703.251.4

2、702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.022邻苯二甲酸氢钾分子量=204.23, 0.2 mol/L邻苯二甲酸氢溶液含40.85克/升3.磷酸氢二钠柠檬酸缓冲液PH0.2mol/LNa2HPO4(亳升)0mol/L柠椽酸 (亳升)PH0.2inol/LNa2HPO4(亳升)O.lmol/L柠檬酸 (亳升)2.20.4010.605.210.729.282.41.2418.765.411.158.852.62817.825.611.608.402.83716.835.812.097.913.04115.896.

3、012.637.373.24.9415.066.213.226.783.45.7014.306.413.85653.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.693510.657.618.731.274.89.8610.147.819.150.855.010.309.7()X.()19.450.55Na田PCM分子量=14.98, 0.2 mol/L溶液为28.40克/升。Na2HPO4 2H2O

4、分子量=178.05, 0.2 mol/L 溶液含 35.01 克/升。C4H2O7 H2O 分子量=210.14, 0.1 mol/L 溶液为 21.01 克/升匚4.柠檬酸-氢氧化钠-盐酸缓冲液PH钠离子浓度（mol/L）柠掾酸（克）C6HsO7 H2O氢氧化钠（克）NaOH 97%盐酸（亳升）HC1 （浓）最终体积 （升）2.20.202108416（）1030.2021083116103.30.2021083106104.30.202108345105.303524514468105.80.45285186105106.50.3S26615612610 使用时可以每升中加入1克克酚，若

5、最后pH值有变化，再用少量50%氢氧化钠溶液或浓盐酸调节，冰箱保存。5.柠檬酸柠檬酸钠缓冲液（O.lmol/L）PH0.1 mol/L 柠樣酸（亳升）0mol/L柠掾酸钠（亳升）PH0mol/L 柠檬酸（亳升）0.1 mol/L柠檬酸钠（亳升）3.01&61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.955.65.514.53.814.06.05.84.71534.013.16.96.03.816.24.21237.76.22.817.24.411.4&66.42.018.04.610.39.76.61.4

6、18.64.89.2KI.S柠檬酸CgHhOt HiO：分子量210.14, 0.1 mol/L溶液为21.01克/升。柠檬酸钠Na3 C6H5O7 2H2O：分子量294.12, 0.1 mol/L溶液为29.41克/亳升。6.乙酸-乙酸钠缓冲液（0.2 mol/L）pH（18°C）0.2 mol/LNaAc（亳升）0.3 mol/LHAc（毫升）pH（18°C）0.2 mol/LNaAc（毫升）0.3 mol/LHAc （亳升）2.60.759.254.85.90403.81.208.805.07.003.004.01.80&205.27.902104.22.6

7、57355.48.601.404.43.706.305.6900.904.64.905.105.S9.400.60Na2Ac 3H2O 分子量=136.09, 0.2 mol/L 溶液为 27.22 克/升。7.磷酸盐缓冲液（1）磷酸氢二钠-磷酸二氢钠缓冲液（0.2）PH0.2 mol/LNa2HPO4 （毫升）0.2 mol/LNaH2PO4 （毫升）PH0.2 mol/LNa2HPO4（毫升）0.2 mol/LNaH2PO4（毫升）5.88.092.07.061.039.05.910.090.07.167.033.06.012387.77.272.02&06.115.085.073

8、77.023.06.21&581.57.481.019.06.322.577.57.584.016.06.426.573.57.687.013.06.531.56&57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.551.0&094.7536.955.（）45.（）Na2HPO4-2H2O 分子量=178.05, 0.2 moVL 溶液为 85.61 克/升。Na田PO412H2O 分子量=358.14, 0.2 mol/L 溶液为 71.64 克/升。NaH2PO4-2H2O 分子量=156.01,

9、 0.2 mol/L 溶液为 31.21 克/升。（2）磷酸氢二钠磷酸二氢钾缓冲液（1/15 mol/L）PHmol/L/15Na2HPO4（亳升）mol/L/15KH2PO4（毫升）PHmol/L/15Na2HPO4（亳升）mol/L/15KH2PO4（亳升）4.920.109.907.177.003.005.290.509.50738&002.005.911.009.007.739.001.006.242.008.008.049.500.506.473.007.008.349.750.256.644.006.00&679.900.106.815.005.008.1810.0

10、006.986.0（）4.0（）Na田PO4-2H2O 分子量=17&05, 1/15M 溶液为 11.876 克/升。KH2PO4 分子量=136.09, 1/15M 溶液为 9.078 克/升。&磷酸二氢钾氢氧化钠缓冲液（0.05M）X亳升0.2M K2PO4+ Y亳升0.2N NaOH加水稀释至29亳升pH（20°C）X （亳升）Y （亳升）pH（20C）X （亳升）Y （亳升）5.850.3727.052.9636.050.5707.253.5006.250.8607.453.9506.451.2607.654.2806.651.7807.854.5206.8

11、52.365&（）54.6809.巴比妥钠-盐酸缓冲液（189）PH0.04M巴比妥 钠溶液0.2V盐酸PH0.04M巴比 妥钠溶液（亳 升）0.2N盐酸（亳 升）6.81001&4&41005.217.010017.88.61003.827.210016.78.81002.527.41001539.01001.657.610013.49.2100137.810011.479.41000.70&01009399.61000.35&21007.21100巴比妥钠盐分子M=206.18;0.04M溶液为&25克/升10. Tris-盐酸缓冲液（0.0

12、5M, 259）50亳升0.1M三疑甲基氨基甲烷（Tris）溶液与X亳升0.1N盐酸混匀后，加水稀释至100 亳升。PHX（亳升）PHX（亳升）7.1045.78.1026.27.2044.78.2022.97.3043.483019.97.4042.0&4017.27.5040.38.5014.77.6038.5&6012.47.7036.68.7010.37.8034.58.80&57.9032.08.907.08.0029.2三疑甲基氨基甲烷(Tris) HOCH2CH2OHCHOCH2 NH2分子 #=121.14;0. 1M溶液为12114克/升。Tris溶液

13、可从空气中吸收二氧化碳，使用时注意将瓶盖严。11.硼酸硼砂缓冲液（0.2M硼酸根）PH0.05M硼砂（亳升）0.2M硼砂（亳升）PH0.05M硼砂（亳升）0.2M硼酸 （毫升）7.41.09.08.23.56.57.61.58.5&44.55.57.82.0&08.76.04.0&03.07.()9.()&()2.0硼砂 Na2B4O7 H2O子虽：=38L43;005M 溶液（=0.2M ft®酸根）含 19.07 克/升。 硼酸HzBOs扮子M=61.84.0.2M溶液为12.37克/升。硼砂易失去结晶水，必须在带塞的瓶中保存。11.硼酸-硼砂缓冲

14、液（0.2M硼酸根）PH0.05M硼砂（亳升）0.2M硼酸（亳升）PH0.05M硼砂 （亳升）0.2M硼酸 （亳升）7.41.09.08.23.56.57.61.58.5&44.55.57.82.0&08.76.04.0&03.07.()9.()S.02.0硼砂Na2B4O7- 10H2O子量=38143;005M溶液（=02M硼酸根）含19.07克/升。硼酸H2BOj,分子量=61.84. 0.2M溶液为12.37克/升。硼砂易失去结晶水，必须在带塞的瓶中保存。12.甘氨酸-氢氧化钠缓冲液（0.05M）X亳升0.2M甘氨酸+Y亳升02NnaOH加水稀释至200亳升PH

15、XYPHXY&6504.09.65()22.48.8506.09.85027.29.0508.810.05032.09.25012.010.4503&69.45016.S1«).65045.5甘氨酸分子M=75.07;0.2M溶液含15.01克/升。13硼砂-氢氧化钠缓冲液（0. 05M硼酸根）X亳升0.05M硼砂+Y亳升02NNaOH加水稀释至200亳升PHXYPHXY9.3506.09.85034.09.45011.010.05043.09.65023.()10.15046.0硼砂 Na2BXh lOHzO.分子M=381.43;0.05M 溶液为 19.07 克/升。14.碳酸钠-碳酸氢钠缓冲液（01M）Ca2+. Mg?+存在时不得使用PH0.1 MNa2CO3(亳升)0.1 MN2HCO3(< 升)20°C37 °C9168.77199.409.12289.519.40379.789.