2022年细胞工程期末复习

1、- 各类专业好文档，值得你下载，训练，治理，论文，制度，方案手册，应有尽有-一. 挑选题细胞工程期末复习资料 1. rnainterference rnairefers to a phenomenon that inhibits expression of genes homologous to that .a: single-stranded dna,ssdnab: double-stranded rna,dsrnac: single-stranded rna,ssrnad: double-stranded dna,dsdna 2. the nobel prize in physiology

2、 or medicine for 2007 was awarded jointly to mario r. capecchi,martin j. evans andoliver smithies, for their discoveries of .a: principles for introducing specific gene modifications in mice by the use of embryonic stem cellsb: the bacterium helicobacter pylori and its role in gastritis and peptic u

3、lcer disease c: rna interference - gene silencing by double-stranded rnad: human papilloma viruses and human immunodeficiency virus3. the nobel prize in physiology or medicine for 2021 was awarded to scientists elizabeth h. blackburn, carol w. greider, and jack w. szostak for their work in the disco

4、very of .a. principles for introducing specific gene modifications in mice by the use ofembryonic stem cellsb. how chromosomes are protected by telomeres and the enzyme telomerasec rna interference - gene silencing by double-stranded rnad human papilloma viruses and human immunodeficiency virus4.贴壁型

5、细胞附着在某一固相支持物表面才能生长，贴壁后出现特定的形状特点；如下列图的细胞类型称为.a: 成纤维细胞型b:游走细胞型 c:上皮型细胞 d: 多型细胞型（ ） 5. 关于动物细胞培育要使用血清，以下说法正确选项；a: 血清使用前必需灭活b:到目前为止，全部动物细胞培育都需要使用血清c:血清中含有抗蛋白酶成分， 起到中和作用，可以用血清来终止胰蛋白酶的消化；d: 血清的使用有利于试验和生产的标准化（ ） 6. 目前，全球已有大约多少人通过体外受精技术诞生？a4万b40万c400万d4000万（ ）7. which of the following physical characteristic

6、s did vincent not have to hide or alter in order to pass off as jerome.a.his heightb.eye-colorc.left-handednessd.his accent speech（ ）8. after the mission director was murdered, what did the police find of vincentnear the crime scene that led them to suspect him of the crime.a.his eyelashb.his heart

7、monitorc.his walletd.his fingerprint- 各类专业好文档，值得你下载，训练，治理，论文，制度，方案手册，应有尽有- 各类专业好文档，值得你下载，训练，治理，论文，制度，方案手册，应有尽有-（ ） 9. how did jerome/eugene become paralyzed.a.he was attacked by a drunk in a barb.he fell off a buildingc. he was hit by a card.he was injured in a freak swimming accident（ ） 10. how was

8、 the murderer identified in the end.a. the murderer was never identifiedb. from video surveillance footagec. from saliva found in the victim s eyed. a witness to the murder came forward（ ）11. 在电影千钧一发 （gattaca）中，没有使用以下哪种方法鉴别一个人的身份？a：血液b：尿液c：头发d：漱口水（ ） 12. 在电影千钧一发（ gattaca）中，男主人公掉了什么东西而让警察怀疑自己是凶手？a：头发

9、b：眼睫毛c：胡子d：腿毛（ ） 13. 千钧一发 （ gattaca）是一部叙述有关的电影. a克隆人b环境爱护c基因鄙视d干细胞（ ） 14. what was the maximum age limit that the doctors gave to vincent at hisbirth.a30 yearsb 35 yearsc 40 yearsd 50 years（ ） 15. what do the letters or terms, in the title 'gattaca' represent.a. it is a term meaning that peo

10、ple are discriminated against.b. it is created solely from the characters names.c. it is created from the letters used to label nucleotide bases of dna.（ ） 16. at the end of the movie, jerome/eugene gave vincent an envelope and told him not to open it untilhe was “ upstairs in”space. what did eugene

11、 give to vincent.a.his silver swimming medalb.moneyc.a lock of his haird.a letter thanking vincent17.what is a stem cell.a:a cell that can make copies of itself and make more specialized types of cell b:a cell that helps to fight against infectionsc:a cell that is specializedd:a cell that can produc

12、e all the cell types of the body18.where can scientists obtain stem cells. a: only from an embryob: only from adult tissues c: only from the braind: from an embryo or adult tissues19.a blastocyst isa:a very early stage embryo b:a type of stem cellc:part of the blood system d:a type of brain cell- 各类

13、专业好文档，值得你下载，训练，治理，论文，制度，方案手册，应有尽有- 各类专业好文档，值得你下载，训练，治理，论文，制度，方案手册，应有尽有-20.embryonic stem cells can differentiate into which types of cell. a:only brain stem cells and specialized brain cellsb:all types of specialized cells in the body c:only cells that can produce insulind:only cells that can produc

14、e artificial skin 21.which of the following is not a property of adult stem cells.a:they are found among the differentiated cells in a tissue or an organ.b:they can only differentiated into that particular tissues or organs where they are originally from.c:they can proliferate without differentiatin

15、g for a long period.d:they can differentiate into an intermediate cell type or types call called a progenitor cell before they achieve their fully differentiated state.22.which of the following is not true about stem cell.a:stem cell can specialize into different types of cells and tissues. b: stem

16、cell can retain a copy of itself.c:stem cellisaneuploid, typicallyhavingmanyextra copies ofvarious chromosomes.d: stem cell can divide and differentiate under appropriate condition or environment,when there is presence of certain growth factors and other cell regulation signals.23.whichof the follow

17、ingstatements about reproductive and therapeutic cloning is false.a. reproductive cloningcould be used effectivelyfor repopulating endangered species or to help make breeding of specific animals easier.b. reproductive cloning is a technology used to generate an animal that has the same nuclear dna a

18、s another currently or previously existing animal.c. therapeutic cloning, also called "embryo cloning," is the production of human embryos for use in stem cell research.d. dolly was created by therapeutic cloning.24.what method is used today to clone an animal.a. embryo splittingb. somatic

19、 cell nuclear transferc. blastomere separationd. intracytoplasmic sperm injection（ ） 25. 第六日 （the 6th day ）是一部有关的电影？a克隆人b环境爱护c基因鄙视d干细胞（ ） 26. is a 2000 action film directed by roger spottiswoode and starring arnold schwarzenegger.the action hero plays family man adam gibson, who is cloned against hi

20、s will in the future of 2021.a. gattaca b. avatar c. the 6th day d. the island（ ） 27. which gene transfer technique involves a tiny needle which is used to inject dna into a cell lacking that dna sequence.a) spermatogonial stem cells transplantationb) liposome transferc) microinjection- 各类专业好文档，值得你下

21、载，训练，治理，论文，制度，方案手册，应有尽有- 各类专业好文档，值得你下载，训练，治理，论文，制度，方案手册，应有尽有-d) gene targeting of embryo stem es cells（ ） 28. . genetic engineering manipulates gene products at the level of thea protein.b amino acid.c dna.d rna.（ ） 29. gene targeting is done on aan？a sperm cell. begg cell. cfertilizedovum. d embryo

22、nic stem cell.30. a mule is a sterile hybrid from a and a .a. female horsemale donkeyb. male horsefemale donkeyc. female horsemale monkeyd. female monkeymale horse二填空1.： the other half of the 2021 nobel prize is shared jointlyby a pair of frenchresearchers, frano.ise barr -séinoussi and luc mon

23、tagnier, for their discovery of.the cloning ofenabled studies of its origin and evolution.successful anti-retroviral therapy results in life expectancies for persons with infection now reaching levels similar to those of uninfected people.2.： the 2021 nobel prize in chemistry was awarded to scientis

24、ts who advancedthe use of， an indispensable tool in molecular biology.the man who discovered thegene, however, is doing something quitedifferent these days.with the aid of , researchershave developed ways to watch processesthat were previously invisible, such as the development of nerve cells in the

25、 brain or how cancer cells spread. are used in experiments to observe cell dynamics and behaviour theirfluorescent glow allows scientists to visualise processes inside cells. ： is a relatively new technology utilizing labeled dna probes to detect or confirm gene or chromosome abnormalities that are

26、generally beyond the resolution of routine cytogenetics.3.： is a solution commonly used in biological research.has many uses because it is isotonic and non-toxic to cells. it can be used to dilute substances.4.： when the media is needed, add 50-ml ofand 5-ml of antibiotics to one of the portions. th

27、is completes the media for use.i need to decrease the concentration ofin my media. normally, i use 10% and i would like to use 1%. does it cause any problem for cell growth.5. atcc : 6.： - 各类专业好文档，值得你下载，训练，治理，论文，制度，方案手册，应有尽有- 各类专业好文档，值得你下载，训练，治理，论文，制度，方案手册，应有尽有-the national institutes of health, a p

28、art of the u.s. department of health and human services, is the primary federal agency for conducting and supporting medical research. helping to lead the way toward important medical discoveries that improvepeople's health and save lives,scientists investigate ways to prevent disease as well as

29、 the causes, treatments, and even cures for common and rare diseases. composed of 27 institutes and centers, theprovides leadership and financialsupport to researchers in every state and throughout the world.7.： 管婴儿” 又称“其次代试can be effective when as few as one sperm can be obtained .studies published

30、 in scientific journals have shown that children resulting from are more likely than other children to have a genetic abnormality.8.： the size of a dna piece that can be reproduced inis 10 times greater than that which can be reproduced in bacteria.techniques for cloning genomic dna intomake it poss

31、ible to analyze verylong dna sequences like human genes.9.： in support of the functional analysis of genes, thes are very useful for making transgenic animals with segments of human dnas.is a vector used to clone dna fragments 100- to 300-kb insert size; average,150 kb in escherichia coli cells.三.名词

32、说明1. green fluorescent protein :2. contact inhibition：3. barr body：4. primary culture ：5. sperm capacitation： 6.ermatogenesi：s8.in vitro fertilization ： 9.embryonic stem cells： 10.induced pluripotent stem ce：ll 11.hematopoietic stem cell：12.hematopoietic stem cell transplantatio：n 13.mesenchymal stem cell ： 14.spermatogonial stem cell：s15. tissue engineering：16. gene targeting：17. mammary gland bioreac