病原微生物学教学课件：20-Rabies virus,Human papillomavirus,Poxvirus and human parvovirus B19,Prion

1、Rabies virusHuman papillomavirusPoxvirus and human parvovirus B19PrionSection ARabies virusBiological Properties nRhabdoviridae (弹状病毒科), Lyssavirus (狂犬病病毒属). nSsRNA, enveloped virusnWide host range. nSingle serotype Animal susceptibility wide host range: All warm-blooded animals温血动物nwidely distribut

2、ed in animalsnnervous system nsaliva nurine nlymph nmilk nblood Pathogenesis and Immunity Source： rabid animal患病动物（狂犬） disease ： rabies， hydrophobia 狂犬病（恐水病）an acute，fulminant，fatal encephalitis急性爆发性致死性脑炎 Transmission route： Bite of a rabid animalPathogenesis and Immunity nSpread through nerve syste

3、m.nNegri bodynAcute, fatal encephalitis (致死性脑炎).nThe clinical spectrum can be divided into three phases.The typical lesion in humans brain: Negri bodynonspecific symptoms：malaise，anorexia，headache，photophobia，nausea and vomiting，sore throat，and fever nervous system dysfunction : nervousness, apprehe

4、nsion, hallucination, hydrophobia The development of rabies virus in vivoTreatment and PreventionnNo successful treatment for clinical rabies.nThe mortality of the disease is 100%nimmediate and thorough cleansing 迅速处理伤口 20%皂水70%酒精nrabies immune globulin高效价抗狂犬病毒血清nKilled-virus vaccine is administered

5、 intramuscularly on the day of exposure and the on days 3，7，14，and 28.Section BPAPILLOMAVIRUSES Biological Properties nPapovaviridae family n55 nm in diameter.nDouble-stranded DNA enclosed within a nonenveloped capsid.nArtificial cultivation un-successful.Pathogenesis and Immunity nHighly tropic (高度

6、亲嗜性) for epithelial cells of the skin and mucous membranes.nHPV type 16 and 18 are considered to be high cancer risk.nCell-mediated immunity.Oral Warts Human PapillomavirusesSection EPrionBiological PropertiesnLacks detectable nucleic acidsnPrPsc (scrapie prion protein 羊搔痒症朊粒蛋白) PrPc（cellular prion

7、protein 细胞朊粒蛋白).nUnusually resistant to standard means of inactivation.Biological PropertiesPathogenesis and ImmunityDirect ContactNormal PrionProteinsPrion protein contact recruits normal protein into abnormal conformationsAbnormal PrionProteinNormal PrionProteinsAbnormal PrionProteinPathogenesis a

8、nd Immunity nTransmissible spongiform encephalopathies: a unique group of fatal neuordegenrative disorders致死性中枢神经系统的慢性退化性疾患Long incubation periods（months to decades） 长潜伏期 chronic progressive pathology （weeks to years） 慢性进行性 Clinical findings1. Human Prion Diseases:n(1) Creutzfeldt-Jakob Disease (CJD

9、) (克雅病)n(2) Variant Creutzfeldt-Jakob Disease (vCJD) (克雅病变种)n(3) Gerstmann-Straussler-Scheinker Syndrome (GSS综合征)n(4) Fatal Familial Insomnia (致死性家族性失眠)n(5) Kuru (库鲁病或颤抖病)2. Animal Prion Diseases:n(1) Bovine Spongiform Encephalopathy (BSE) (牛海绵状脑病或疯牛病)n(2) Chronic Wasting Disease (CWD) (慢性消耗性疾病)n(3) Scrapie (羊搔痒病)n(4) Feline spongiform encephalopathy (猫海绵状脑病)n(5) Ungulate spongiform encephalopathy (有蹄类海绵状脑病)Laboratory Diagnosis nThe clinical picturenWe