槐定碱抗骨癌痛作用和其机制探究

1、槐定碱抗骨癌痛作用和其机制探究摘要目的：采用w256癌细胞诱导的骨癌痛模型 大鼠探讨槐定碱抗癌痛药效及其作用机制。方法：采用骨髓 腔注射w256癌细胞复制骨癌痛大鼠模型；造模10 d后选取 模型复制成功的大鼠36只随机分为模型对照组及槐定碱治 疗组，另取同期10只大鼠作为正常对照组；治疗组大鼠于 造模第15天开始给予槐定碱25 mg kg-1治疗10 d；各组 大鼠给药前、后测量机械痛阈和热痛阈，末次给药后，对 大鼠患肢进行放射学和组织病理学观察，并采用免疫组化法 检测大鼠患肢肿瘤组织环氧酶-2 (c0x-2)、血管内皮生长因 子(vegf)的表达。结果：槐定碱可明显提高骨癌痛大鼠机 械痛阈和

2、热痛阈值(p3. 2槐定碱对大鼠热痛阈的影响造模动物在术后第14天出现明显热痛觉超敏，热痛阈值显 著下降(p9 hu m, peluffo g, chenh, et al.role of cox-2 in epithelial-stromal cell interactions and progression of due tai carcinoma in situ of the breast j. proc natl acad sci usa, 2009,106：3372.10 王勤，邓家刚，杨柯，等芒果昔对慢性支气管炎 大鼠炎症因子及小鼠巨噬细胞环氧化酶-2表达的影响j. 中国中药杂志，2

3、011, 36 (10)： 1348.11 sabino m a, ghilardi j r, jongen j l, etal. simultaneous reduction in cancer pain , bone des true tion， and tu mor grow th by selec tive inhib ition of cyclooxygenase-2j. cancer res, 2002,62：734312 王日雄，施烯，林学德，等.塞来昔布在mrmt-1 大鼠乳腺癌骨转移性癌痛模型的作用机制探讨j.中国疼 痛医学杂志，2010, 16 (4)： 228.13 ta

4、wfik 0 w, kramer b, shideler b, et al.prognostic significance of cd44, platelet-derived growth factor receptor alpha, and cyclooxygenase-2 expression inrenal cell carcinomaj. arch pathoi lab med, 2007, 131：261.14 basu g d, pathangey l b, mukherjee p, etal. mechanisms underlying the growth inhibitory

5、 effects of the cyclo-oxygenase-2 inhibitor celecoxib in human breast cancer cellsj breast cancer res, 2005,7：4220.study on effect of sophoridine against bone cancer pain and its mechanismyan ji-gui*, yang yu-qing, wang ya-jie, kan jing(department of pharmacy, anhui medical college, hefei 230601, ch

6、ina)abstract objective ：to study the effect ofsophoridine against bone cancer pain in bone cancer pain model rats induced by w256 tumor cells and its mechanism. method： the rat model of bone cancer pain was reproduced by injecting w256 tumor cells into the rat marrow cavit y.ten days after the model

7、establishment,36 rats were selected and randomlydivided into the model control group and the sophoridine treated group. at the same time, other 10 rats with sham-operation were selected to be the normal control group. since the 15th day after the operation, rats in the treated group had been given s

8、ophoridine (25 mg kgl) for 10 days the mechanical withdrawal threshold and the thermal withdrawal latency of each group were measured before and after the treatment after the last treatment ,the radiological andhistopathological observation shall be conducted for sick legs of all rats.the expressions ofcyclooxygenase-2 (cox-2) and vascular endothelial growth factor (vegf) in tumor tissues were detected by mmunohistoch