人胚胎期发生ppt课件

1、general embryology general embryology (i)(i)1. langmans medical embryology with simbryo cd-rom, lippincott williams & wilkins publishers 9th edition2.高英茂主编，组织胚胎学，全国八年制教材，人民卫生出版社，20063.邹仲之，李继承主编，组织学与胚胎学，人民卫生出版社，20084.组胚精品课程网站：http:/ a science which study the processes and regulations of developme

2、nt of human fetus.(from 1 cell (zygote合子) to (5-7) x1012 cells) total 38 weeks: preembryonic period（胚前期）: before 2 weeksembryonic period（胚期）: 3-8 weeks fetal period（胎儿期）: after 9 weeks fertilization（受精） definition: the process by which the male and female gametes(sperm and ovum) unite to give rise t

3、o zygotesite: in the ampullary region（壶腹部） of the oviductconditions: 1) maturation of oocyte 2) maturation of spermatozoa 3) capacitation （获能）（获能）and acrosome reaction（顶体反应）（顶体反应） of spermatozoa capacitation factor: secreted by epi. cells of oviduct, capacity of releasing acrosome enzymes4) quality

4、and quantity of spermatozoa: 2-6 ml, 100,000,000/ml, 1.5 ml; or 30%; or capacity for mobile 70% 5) meeting of sperm and ovum within 24 hrs processes of fertilization:phase i penetration of the corona radiata（放射冠）, acrosomal releasephase iipenetration of the zona pellucide（透明带）zp with receptors zona

5、pellucida spongy the release of local enzymesphase iiientrance, formation of zygoteentry response1)cortical or zona reactions -compaction - impermeable to more sperm2)oocyte finishes 2nd meiotic division3)metabolic activation of the eggresults of fertilizationdiploidgenetic sexcleavagecleavage （卵裂）（

6、卵裂）mitosistwo cell stage (30h)morula（桑椹胚） (72h)inner cell massembryoblastouter cell rimtrophoblastsblastocyst （胚泡）(5d)blastocoelethe cells of morula secrete fluidzona pellucide disappearsblastocyst （胚泡）（胚泡）inner cell massblastocoeletrophoblasts（滋养层）（胚泡腔）（内细胞群）puberty menstruation (28 days) hormonal

7、regulationgnrh fshlhgranulosa/theca cellsestrogens progesteronesthe endometrium of the uterus is in the secretory phaseuterus at time of implantationimplantation（植入）-passage through the uterine tubeday 5 day 11 uterusorientation of implantationlocation - ant or post, fundustrophoblasts overlying the

8、 embryoblast pole attach to the endometrium and begin to invade.processes zona pellucide disappear polar trophoblast touch the endometriumsecrete enzymes dissolve the endometrium embedded into endometrium coagulation plug seal the spaceimplantation sitenormal:ant or post wall of the uterus, fundusab

9、normal:internal os of the cervix (placenta previa)ovarianabdominaltubalectopic pregnancyendometrium decidua: -decidua basalis （底蜕膜）（底蜕膜） -decidua capsularis（包蜕膜）（包蜕膜） -decidua parietalis（壁蜕膜）（壁蜕膜）conditions: -endometrium is in secretory phase -morula reach the cavity of uterus on time -zona pellucid

10、e disappears in timein vitro fertilisation体外受精联合胚胎移植技术（ivf）：又称试管婴儿，是指分别将卵子与精子取出后，置于试管内使其受精，再将胚胎前体-受精卵移植回母体子宫内发育成胎儿的过程。 生育年龄不孕妇女，具备下列任何一项时： 1经碘油造影、腹腔镜术或手术证实，各种原因引起的盆腔粘连或输卵管阻塞。 2双侧输卵管切除术后或结扎术后复通失败或不愿接受复通手术。 3盆腔子宫内膜异位症，反复促排卵治疗失败。 4顽固性多囊卵巢综合征，经反复促排卵治疗失败。 5不明原因不孕，反复促排卵治疗失败。 preimplantation genetic diagno

11、sis植入前遗传学诊断(pgd)从发育到4-8细胞的胚胎中，取出一个细胞进行细胞遗传学 或分子遗传学检查，再将检查结果正常的胚胎放入子宫内, 以达到优生优育目的。 目前主要用于已知患有遗传性疾患夫妇或高风 险遗传疾患携带者（如高龄妇女）。 implantation - sinks deeperthe outer cell layertrophoblastscytotrophoblasts 细胞滋养层细胞滋养层syncytiotrophoblasts 合体滋养层合体滋养层inner cell mass (embryoblast)fluid collection amnionepiblasts &

12、amp; hypoblasts bilaminar germ disc （二胚层胚盘） (second week of development)week two day 9-12implantationcovered the endometrium- fibrin plugcomplete slight bulgetrophoblastsvacuoles within syncytiotrophoblasts- fuse and form lacunar networksuteroplacental circextra-embryonic mesodermbtwn troph & cavitysomatic/splanchnicweek 2 day 13-14trophoblastsprimary villiyolk sacprimary to secondarychorionic plateconnecting stalkthe bilaminar embryodisc btwn amnion（羊膜腔）yolk sac（卵黄囊）bilaminar discepiblasts（上胚层）hypoblasts