新建文件夹catalogue cellules Dimitri

1、NOMTYPE TISSUESPECEindication de culture ATCC/ECACCmilieusrumA204myosarcome but NO has recently been reclassified as an undifferentiated sarcoma;a rhabdoid linemusclehommeMcCoys 5a10%A375mlanomepeauhommeDMEM15%A431mlanomepeauhommeEMEM 10%A549carcinomepoumonhommeF-12K 10%CACO-2adenocarcinomecolonhomm

2、eEMEM 10%DAUDI lymphome de BurkittLBhommeRPMI 164010%HeLacarcinomeutrushommeEMEM 10%HEP-G2carcinomefoiehommeEMEM 10%HOSsarcomeoshommeEMEM 10%HT1080sarcomefibroblastehommeEMEM 10%HUH 7.5carcinomefoiehommeDMEM10%H358carcinomebronchioalvolehommeRPMI 164010%JURKATlymphobastomeLThommeRPMI 164010%MOLTleuc

3、mie LThommeRPMI 164010%RAJIlymphome de BurkittLBhommeRPMI 164010%TE671sarcomefibroblastehommeEMEM 10%THPleucmie monocytehommeRPMI 164010%indication de culture du labo glutamineautremilieusrumglutamineautreRPMI 1640 supplemented with 10% fetal bovine serum at 37C in 5% CO22mMDMEM10% 2mM2mMNEAA 1%DMEM

4、10% 2mM2mMDMEM10% 2mM2mMNEAA 1%DMEM20% 2mMNEAA 1% + HEPES 1%2mMRPMI 164010% 2mM2mMNEAA 1%DMEM10% 2mM2mMNEAA 1%DMEM10% 2mMSur collagne type I (prparer un stock 1/50 et coater les boites 15min; recycler le PBS-Collagen)2mMNEAA 1%2mMNEAA 1%DMEM10% 2mM2mMDMEM10% 2mM2mMRPMI 164010% 2mM2mMRPMI 164010% 2mM

5、2mMRPMI 164010% 2mM2mMRPMI 164010% 2mM2mMNEAA 1%DMEM10% 2mM2mMRPMI 164010% 2mMindication de culture ATCC/ECACCSlection concentrationG418Blasticydine Zeomycine HygromycineA204 was previously (but no longer) listed in the ATCC catalog as an RMS cell line. However in 1998, A204 cells were found to lack

6、 expression of myogenin, MyoD, or desmin, suggesting that either they were not of RMS origin or that with passaging in culture there was down-regulation of myogenic genes (23). These observations were especially compelling, since a prior immunohistochemical study found that most RMS tumor samples (b

7、ut not normal adult muscle including skeletal muscle) expressed MyoD (75). In 2002, A204 cells were found to have a SMARCB1 (SNF5) mutation, consistent with its classification as a rhabdoid line (76).Sur collagne type I (prparer un stock 1/50 et coater les boites 15min; recycler le PBS-Collagen)A204

8、 was previously (but no longer) listed in the ATCC catalog as an RMS cell line. However in 1998, A204 cells were found to lack expression of myogenin, MyoD, or desmin, suggesting that either they were not of RMS origin or that with passaging in culture there was down-regulation of myogenic genes (23

9、). These observations were especially compelling, since a prior immunohistochemical study found that most RMS tumor samples (but not normal adult muscle including skeletal muscle) expressed MyoD (75). In 2002, A204 cells were found to have a SMARCB1 (SNF5) mutation, consistent with its classificatio

10、n as a rhabdoid line (76).A204 was previously (but no longer) listed in the ATCC catalog as an RMS cell line. However in 1998, A204 cells were found to lack expression of myogenin, MyoD, or desmin, suggesting that either they were not of RMS origin or that with passaging in culture there was down-re

11、gulation of myogenic genes (23). These observations were especially compelling, since a prior immunohistochemical study found that most RMS tumor samples (but not normal adult muscle including skeletal muscle) expressed MyoD (75). In 2002, A204 cells were found to have a SMARCB1 (SNF5) mutation, con

12、sistent with its classification as a rhabdoid line (76).A204 was previously (but no longer) listed in the ATCC catalog as an RMS cell line. However in 1998, A204 cells were found to lack expression of myogenin, MyoD, or desmin, suggesting that either they were not of RMS origin or that with passagin

13、g in culture there was down-regulation of myogenic genes (23). These observations were especially compelling, since a prior immunohistochemical study found that most RMS tumor samples (but not normal adult muscle including skeletal muscle) expressed MyoD (75). In 2002, A204 cells were found to have

14、a SMARCB1 (SNF5) mutation, consistent with its classification as a rhabdoid line (76).A204 was previously (but no longer) listed in the ATCC catalog as an RMS cell line. However in 1998, A204 cells were found to lack expression of myogenin, MyoD, or desmin, suggesting that either they were not of RM

15、S origin or that with passaging in culture there was down-regulation of myogenic genes (23). These observations were especially compelling, since a prior immunohistochemical study found that most RMS tumor samples (but not normal adult muscle including skeletal muscle) expressed MyoD (75). In 2002,

16、A204 cells were found to have a SMARCB1 (SNF5) mutation, consistent with its classification as a rhabdoid line (76).A204 was previously (but no longer) listed in the ATCC catalog as an RMS cell line. However in 1998, A204 cells were found to lack expression of myogenin, MyoD, or desmin, suggesting t

17、hat either they were not of RMS origin or that with passaging in culture there was down-regulation of myogenic genes (23). These observations were especially compelling, since a prior immunohistochemical study found that most RMS tumor samples (but not normal adult muscle including skeletal muscle)

18、expressed MyoD (75). In 2002, A204 cells were found to have a SMARCB1 (SNF5) mutation, consistent with its classification as a rhabdoid line (76).A204 was previously (but no longer) listed in the ATCC catalog as an RMS cell line. However in 1998, A204 cells were found to lack expression of myogenin, MyoD, or desmin, suggesting that either they were not of RMS origin or that with passaging in culture there was down-regulation of myogenic genes (23). These observations were especi