工业化生物发酵技术——清华大学国际技术转移中心

1、Biotechnology Catalogue Version Note: Most of the following technologies are for A.P.I.s, and they have been successfully commercialized in the market. www.coway-工业化生物发酵技术（原料药）Product名称Guaranteed Values指标ACARBOSE阿卡波糖Final titre: 8-9g/lt Fermentation time: 120-144hrsRecovery yield: 55%ACLARUBICIN 阿柔比

2、星 Two step fermentationACRYLAMIDE 丙烯酰胺Final titre: 325 g/ltFermentation time: 40-48 hrsRecovery yield: 98%AMOXICILLIN 阿莫西林Yield: 90%(guaranteed; semi-continuous process with recycling) 95%(achieved; semi-continuous process with recycling)Amphotericin B两性霉素BFinal titre: 10000U/ml(HPLC)Fermentation ti

3、me: 130-140hrsRecovery yield: 70%ANDROSTENDIONE-SPIRONOLACTONE 雄烯二酮-螺内酯Androstendione Final titre: 20 2 g/ltFermentation time:96 24 hrsRecovery yield:90 % as Crystals 96% puritySprironolactone Synthesis:66% from ADAPRAMYCIN Sulfate 硫酸安普霉素Final titre: 8 g/lFermentation time: 144 12 hrsRecovery yield:

4、 80%ARACHIDONIC ACID 花生四烯酸Final titre: 10 1 g/ltFermentation time: 134 hrsRecovery yield: 80 5%ARGININE精氨酸 Final titre: 755g/LFermentation time: 50 hrsRecovery yield: 80 as crystal 90 feed gradeASTAXANTHIN 虾青素Final titre: 3 g/ltFermentation time: 18010 hrsRecovery yield: 60%AVERMECTIN阿维菌素 Final titr

5、e: 5000 u/ml (+ 10%) Fermentation time: 260-280 hrsRecovery yield: 75%BACITRACIN-Zinc 杆菌肽锌Final titre1200u/mLFermentation time:36 hrsRecovery yield:55 %BLEOMYCIN 博莱霉素Final titre: 14020mg/LFermentation Time: 14412hrsRecovery yield: 35%CABERGOLINE卡麦角林By chemical synthesis from Lysergic acid3.29kg Cabe

6、rgoline from 1kg Lysergic acidb-CAROTENE b-胡萝卜素Final titre: 3g/lFermentation time: 9612 hrsRecovery yield as feed quality:90 %CEPHALOSPORIN C, Na SALT头孢菌素C钠盐Final titre: 28 g/ltPilot plant titer: 30 g/ltFermentation time: 14424 hrsRecovery yield: 80%CHLORTETRACYCLINE金霉素 Final titre: 33 g/L Fermentat

7、ion time: 100 hrs Recovery yield: 94 % feed grade, 75 % hydrochlorideCitric Acid柠檬酸The yield，conversion rate and fermentation time are different between using different kinds of raw materials.CLAVULANIC ACID 克拉维酸Final titre: 111g/ltFermentation time: 140 12 hrsRecovery Yield: 605 %COMPACTIN美伐他汀Final

8、 titre: 14 g/lCYCLOSPORIN A 环孢霉素AFinal titre: 5 1 g/lt Fermentation time: 168 hrsRecovery yield: 70 %DALBAVANCIN PRECURSOR达巴万星（道古霉素）前体Final titre: 1 0.2 g/lt Fermentation time: 21612 hrsRecovery yield: 50 %DAUNOMYCIN 柔红霉素Final titre: 3g0.3g/ltFermentation time: 16812 hrsRecovery yield: 67 %DAPTOMYCI

9、N/CUBICIN 达托霉素 Final titre: 2.50.3 g/ltFermentation time: 19624hrsRecovery yield Pharma grade: 40%DESFEROXAMIN去铁胺Final titre: 15 1.5 g/lt Fermentation time: 19212 hrsRecovery yield: 35 - 40 %DHAFinal titre: 28 3 g/lt Fermentation time: 12024 hrsRecovery yield: 80 %DMCT/Demethylchlortetracycline去甲基氯四

10、环素/去甲金霉素Final titre: 10.0 1g/LFermentation time: 300 hrs Recovery Yield: 75 DORAMECTIN 多拉霉素 Final titre: 1500UI/mlFermentation time: 200 hrs Recovery Yield: 75DOXORUBICIN阿霉素Antitumor drugChemical synth. from Dauno: Yield 50%ENRAMYCIN 恩拉霉素Final Titre: 10g/ltFermentation Time: 260hrsRecovery yield: 92

11、%ERYTHROMYCIN 红霉素 Final titre (HPLC): 10 0.5g/lt Fermentation time: 168+24 hrsRecovery yield: 85% as Thiocyante. 82% as Base EPIRUBICIN表阿霉素Antitumor drugChemical synth. from Dauno: Yield 30%EPOTHILONE B 埃博霉素BFinal titre: 70-90 mg/LFermentation time: 168-216 hrsFLAVOMYCIN黄霉素Final titre: 14 1 g/lt Fer

12、mentation time:148 - 172 hrsRecovery yield as feed quality:92 %Fusidic Acid 福西地酸 Final Titre: 3.8g/LFermentation Time: 190hrsRecovery yield: 60%FRUCTO-OLIGO SACCARIDE (FOS) 果寡糖Contact us for more detailsGENTAMYCIN 庆大霉素Final titre: 6g/l Fermentation time: 14daysRecovery yield: 80%Glutathione(GSH) 谷胱苷

13、肽Final titre: 5.50.5 g/lFermentation time: 655 hrsRecovery yield: 45-50%GUANOSINE 鸟苷Final titre: 50-60 g/lFermentation time: 72 hrsSugar conversion rate:25%Recovery yield: 80%GIBBERELLIN (GA) 赤霉素Fermentation loading coefficient：9095%Fermentation titre： mix constituents：2200-2400g/ml； GA3 constituent

14、s：19002100g/mlExtraction yield：85%HYALURONIC ACID透明质酸Final titre: 6.5 g/litre Fermentation time: 20-24 hrsRecovery yield: 90 % Cosmetic GradeINOSINE 肌苷Final titre: 90 g/lFermentation time: 85-90 hrsSugar conversion rate:35%Recovery yield: 80%KANAMYCIN 卡那霉素Final titre:30g/lFermentation time:300 hrsRe

15、covery yield: 80%KASUGAMYCIN 春雷霉素Final titre: 10000-12000U/mlFermentation time:8 daysRecovery yield: 80%LACTIC ACID 乳酸Final titre:120 10g/ltFermentation time:72 10 hrsRecovery yield:90%LINCOMYCIN 林肯霉素Final titre:8 g/ltFermentation time:192 24 hrsRecovery yield： Pharma grade 80% Feed Grade 90% LIPSTA

16、TIN 泥泊司他汀Chemical conversion from Lip to OrlistatORLISTAT 奥利司他Final titre:8 1 g/ltFermentation time:168 hrsRecovery yield:75%Contact us for more details.LOVASTATIN 洛伐他汀Final titre 17g/lt Fermentation time 264 24 hrsRecovery yield 70 5 %Lycopene番茄红素Titre： 2.5 0.5 g/lFermentation time: 12012 hrs Extra

17、ction yield: 755%LYSERGIC ACID 麦角酸Final titre 6 g/lFermentation time: 360 hrsRecovery yield: 70 %LYSINE 赖氨酸Final titre:190g/lFermentation time:45 hrsRecovery yield: 95 % for crystal (assay 98.5%) 100% for feed grade (assay 65%)Sugar conversion rate: 55% - 60 %MADURAMYCIN 马杜拉霉素Final titre: 10000 1200

18、0 UI/mlFermentation time 180 210 hrs.Recovery yield: 90 % Feed GradeMIBEMYCIN 美贝霉素Final titre: 400 U/mlExtraction yield: 60%-70%Synthetic yield: 30%-50%MITHRAYCIN光辉霉素Production Titre： 500 mg/lMONENSIN 莫能菌素Final titre: 32000-40000 u/mlFermentation time: 320 hrs.Recovery yield: 95%MUPIROCIN 莫匹罗星Final

19、Titre： 3000mg/L（50L fermentor）Fermentation Time： 60hrsMYCOPHENOLIC ACID 麦考酚酸Final titre: 161 g/lFermentation time: 31224hrsRecovery yield: 68 % NATAMYCIN 纳他霉素Final titre: 8g/l(Maximum 10g/l)Fermentation time: 140-160 hrs.Recovery yield: 55-65%NICERGOLINE 尼麦角林By chemical synthesis from Lysergic acid2

20、.47kg nicergoline from 1kg Lyserig acidNYSTATIN制霉菌素Final titre: 400,000 IU/mlFermentation time: 32424 hrs.Recovery yield:85 % Feed Grade, 70 % Pharma GradeOXYTETRACYCLINE土霉素Final titre:33mg/lFermentation time:240hrsRecovery yield feed grade:94%PARAMOMYCIN巴龙霉素Final titre:6g/ltFermentation time:156 hr

21、sRecovery yield:70 % as CrystalsPENICILLIN G POTASSIUM SALT青霉素G钾盐 Final titre:80,000 IU/mlFermentation time:192 hrsRecovery yield: 85 %Phenylalanine苯丙氨酸Final titre: 322glFermentation time:45-55 hrsRecovery yield: 75Conversion rate: 14-16 PLEUROMUTILIN截短侧耳素Final titre: 13 g/lt（50L fermenter）Pneumocan

22、din Bo 肺囊康定 New Chemical conversion from Bo to caspofunginCASPOFUNGIN 卡泊芬净Final titre:2.3-3.0g/LFermentation time:312 hrsRecovery yield: 70 %Contact us for more details.MICAFUNGIN 米卡芬净Contact us for more details-Polylysine 聚赖氨酸 Yield of acid by fermentation: 20g/l Fermentation time： 70hrsRecovery yi

23、eld： 80POLYMYXIN 粘杆菌素BFinal titre: 2.5 0.2 g/lFermentation time:16824 hrsRecovery yield:70 %POLYMYXIN E Sulfate硫酸粘杆菌素E Final titre:450.000 U/l (Maximum 560,000U/l)Fermentation time96 hrs10Recovery yield: 93-95% concentrate 80-85% A.P.I.PRAVASTATIN 普伐他汀 Titre: 15 g/lFermentation time: 9-10daysRecover

24、y yield:65 %Conversion from Mevastatin : 70%Prednisolonum/ Prednisolone氢化泼尼松/泼尼松龙 Concentration of substrate: 20 g/L (Maximum 70g/L)Bioconversion time: 5-8 hrsSubstrate conversion rate: 97100Yield of crystalline final product: 80PRISTINAMYCINS普那霉素/原始霉素Final titre: 2.5- 3.0g/L Fermentation time:64 hr

25、s.Recovery yield: 75%COENZYME Q10 辅酶Q10 Final titre:3.50.5g/lFermentation time:12012 hrsRecovery yield:50 %Ramoplanin雷莫拉宁Final titre: 20.2 g/LFermentation time: 21612 hrsRecovery yield: 50%RIFAMYCIN-RIFAMPICIN 利福霉素-利福平Final titre: 26 g/lt Fermentation time: 192 hrsRecovery (up to Rifampycin): 65%Rh-

26、 LACTOFERRIN乳铁蛋白Final titre: 3.50.3g/LFermentation time: 16812hrsRecovery yield: 605%SAMe 腺苷蛋氨酸Final titre: 10g/LFermentation time: 16 hrsSALINOMYCIN 盐霉素Final titre: 10 g/lt Fermentation Time: 360 hrs 24Recovery as Feed Grade: 92 %SHIKIMIC ACID莽草酸Final titre: 30 3 g/ltFermentation time:60 12 hrsReco

27、very yield: 75%SIMVASTATIN 辛伐他汀Chemical conversion of Lovastatin11 12 kg Simvastatin from 20 kg LovastatinSIROLIMUS 西罗莫司Final titre: 1.2 0.1 g/ltFermentation time:216 24 hrsRecovery yield: 50%SPECTINOMYCIN 大观霉素Final titre: 4000u/ml Recovery yield: 65-70 %SPIRAMYCIN/Rovamycin 螺旋霉素Final titre: 5000r/m

28、 Fermentation Time: 144 hrs Recovery yield: 76% STREPTOMYCIN 链霉素Final titre: 24 g/lFermentation time: 200 hrsRecovery yield: 72 %TACROLIMUS 他克莫司 Final titre: 1.2 g/lFermentation time: 240 + 24hrsRecovery yield: 70 %TEICOPLANIN替考拉宁Final titre: 5+ 0.5 g/lFermentation time: 168 + 12 hrsRecovery: 55 %Te

29、stosterone睾丸激素Concentration of substrate: 5.0 g/LBioconversion time: 135145 hrscontent in cultivation broth: 4850Yield of crystalline final product: 3035TETRACYCLINE HCI 盐酸四环素Final titre: 30 g/lFermentation time: 200 hrsRecovery yield: 74 %THREONINE苏氨酸Final titre: 85-95 g/lFermentation time: 35-40 h

30、rsRecovery: 80%THIAMULIN Base泰妙菌素碱 Conversion rate: From 1 kg Pleuromutilin to 1,23 kg Thiamulin FumarateTOBRAMYCIN 妥布霉素Final titre: 6000r/mlFermentation time: 120-144 hrsRecovery yield: 30 % TRYPTOPHAN 色氨酸 Final titre:353 g/LFermentation time:505 hrsRecovery yield as crystal: 70%Recovery yield as F

31、eed Grade: 85%Glucose conversion rate: 12-15TYLOSIN TARTRATE 酒石酸泰乐菌素Final titre: 15 1 g/lt. Fermentation time: 18012 hrsRecovery yield: 70% VALINE 缬氨酸 Final titre: 605 g/lFermentation time: 60 5 hrsRecovery yield Pharma grade: 65% VANCOMYCIN 万古霉素Final titre: 12 1 g/lFermentation time: 180 12hrsRecov

32、ery yield: 70 %VITAMINE B12 维生素V B12Final titre: 220 g/LFermentation time: 168 hrsRecovery yield: 70% as Crystal, 80% as Feed GradeVITAMINE B2 Final titre: 33 3g/lFermentation time: 48 24 hrsRecovery yield: 65 % as Pharma Grade, 80 % as Feed GradeXANTHAN 黄原胶Final titre: 25 kg/m3Recovery yield: 80 %F

33、ermentation Time36 hrsAMPICILLIN氨苄青霉素 (CAS 69-53-4) Bio-Enzymatic Reaction CEPHALEXIN头孢氨苄(CAS 15686-71-2)Bio-Enzymatic ReactionCEPHADROXIL 头孢羟氨苄(CAS 50370-12-2)Bio-Enzymatic ReactionD-p-OH-Phenylglycine-amideBio-Enzymatic ReactionD-p-OH-Phenylglycine-esterBio-Enzymatic ReactionL-tert-leucine L-亮叔氨酸Biocatalytic methodL-homophenylalanine L-高苯丙氨酸Biocatalytic methodTECHNOLOGIES OF ENZYMES PRODUCTION（HIGH） ALKALINE PROTEASE 碱性蛋白酶Final tit