常用缓冲溶液的配制方法VIP

1、常用缓冲溶液的配制方法1 甘氨酸盐酸缓冲液（0.05mol/l）x毫升0.2 mol/l甘氨酸+y毫升0.2 mol/l hci，再加水稀释至200毫升phxyphxy2.02.42.62.85050505044.032.424.216.83.03.23.43.65050505011.48.26.45.0甘氨酸分子量 = 75.07，0.2 mol/l甘氨酸溶液含15.01克/升。2邻苯二甲酸盐酸缓冲液（0.05 mol/l）x毫升0.2 mol/l邻苯二甲酸氢钾 + 0.2 mol/l hcl，再加水稀释到20毫升ph(20)xyph(20)xy2.22.42.62.83.0555554.0

2、703.9603.2952.6422.0223.23.43.63.855551.4700.9900.5970.263邻苯二甲酸氢钾分子量 = 204.23，0.2 mol/l邻苯二甲酸氢溶液含40.85克/升3磷酸氢二钠柠檬酸缓冲液ph0.2mol/l na2hpo4（毫升）0.1mol/l柠檬酸（毫升）ph0.2mol/l na2hpo4 （毫升）0.1mol/l柠檬酸（毫升）2.22.42.62.83.03.23.43.63.84.04.24.44.64.85.00.401.242.183.174.114.945.706.447.107.718.288.829.359.8610.3010.

3、6018.7617.8216.8315.8915.0614.3013.5612.9012.2911.7211.1810.6510.149.705.25.45.65.86.06.26.46.66.87.07.27.47.67.88.010.7211.1511.6012.0912.6313.2213.8514.5515.4516.4717.3918.1718.7319.1519.459.288.858.407.917.376.786.155.454.553.532.611.831.270.850.55na2hpo4分子量 = 14.98，0.2 mol/l溶液为28.40克/升。na2hpo42h

4、2o分子量 = 178.05，0.2 mol/l溶液含35.01克/升。c4h2o7h2o分子量 = 210.14，0.1 mol/l溶液为21.01克/升。4柠檬酸氢氧化钠-盐酸缓冲液ph钠离子浓度（mol/l）柠檬酸（克）c6h8o7h2o氢氧化钠(克)naoh 97%盐酸（毫升）hcl（浓）最终体积（升）2.23.13.34.35.35.86.50.200.200.200.200.350.450.3821021021021024528526684838383144186156160116106456810512610101010101010 使用时可以每升中加入1克克酚，若最后ph值有变

5、化，再用少量50%氢氧化钠溶液或浓盐酸调节，冰箱保存。5柠檬酸柠檬酸钠缓冲液（0.1 mol/l）ph0.1 mol/l柠檬酸（毫升）0.1 mol/l柠檬酸钠（毫升）ph0.1 mol/l柠檬酸（毫升）0.1 mol/l柠檬酸钠（毫升）3.03.23.43.63.84.04.24.44.64.818.617.216.014.914.013.112.311.410.39.21.42.84.05.16.06.97.78.69.710.85.05.25.45.65.86.06.26.46.68.27.36.45.54.73.82.82.01.411.812.713.614.515.316.217.

6、218.018.6柠檬酸c6h8o7h2o：分子量210.14，0.1 mol/l溶液为21.01克/升。柠檬酸钠na3 c6h5o72h2o：分子量294.12，0.1 mol/l溶液为29.41克/毫升。6乙酸乙酸钠缓冲液（0.2 mol/l）ph(18)0.2 mol/l naac(毫升)0.3 mol/l hac(毫升)ph(18)0.2 mol/lnaac(毫升)0.3 mol/lhac(毫升)2.63.84.04.24.44.60.751.201.802.653.704.909.258.808.207.356.305.104.85.05.25.45.65.85.907.007.90

7、8.609.109.404.103.002.101.400.900.60na2ac3h2o分子量 = 136.09，0.2 mol/l溶液为27.22克/升。7磷酸盐缓冲液（1）磷酸氢二钠磷酸二氢钠缓冲液（0.2）ph0.2 mol/l na2hpo4(毫升)0.3 mol/l nah2po4(毫升)ph0.2 mol/l na2hpo4(毫升)0.3 mol/l nah2po4(毫升)5.85.96.06.16.26.36.46.56.66.76.86.98.010.012.315.018.522.526.531.537.543.549.555.092.090.087.785.081.577

8、.573.568.562.5 56.551.045.07.07.17.27.37.47.57.67.77.87.98.061.067.072.077.081.084.087.089.591.593.094.739.033.028.023.019.016.013.010.58.57.05.3na2hpo42h2o分子量 = 178.05，0.2 mol/l溶液为85.61克/升。na2hpo412h2o分子量 = 358.22，0.2 mol/l溶液为71.64克/升。na2hpo42h2o分子量 = 156.03，0.2 mol/l溶液为31.21克/升。（2）磷酸氢二钠磷酸二氢钾缓冲液（1/

9、15 mol/l）phmol/l/15na2hpo4(毫升)mol/l/15kh2po4(毫升)phmol/l/15na2hpo4(毫升)mol/l/15kh2po4(毫升)4.925.295.916.246.476.646.816.980.100.501.002.003.004.005.006.009.909.509.008.007.006.005.004.007.177.387.738.048.348.678.187.008.009.009.509.759.9010.003.002.001.000.500.250.100na2hpo42h2o分子量 = 178.05，1/15m溶液为11.

10、876克/升。kh2po4分子量 = 136.09，1/15m溶液为9.078克/升。8磷酸二氢钾氢氧化钠缓冲液（0.05m）x毫升0.2m k2po4 + y毫升0.2n naoh加水稀释至29毫升 ph(20)x (毫升)y（毫升）ph(20)x (毫升)y（毫升）5.86.06.26.46.66.85555550.3720.5700.8601.2601.7802.3657.07.27.47.67.88.05555552.9633.5003.9504.2804.5204.6809巴比妥钠-盐酸缓冲液（18）ph0.04m巴比妥钠溶液0.2v盐酸ph0.04m巴比妥钠溶液(毫升)0.2n盐酸

11、(毫升)6.87.07.27.47.67.88.08.210010010010010010010010018.417.816.715.313.411.479.397.218.48.68.89.09.29.49.61001001001001001001001005.213.822.521.651.130.700.35巴比妥钠盐分子量=206.18;0.04m溶液为8.25克/升10tris盐酸缓冲液（0.05m，25）50毫升0.1m三羟甲基氨基甲烷（tris）溶液与x毫升0.1n盐酸混匀后，加水稀释至100毫升。phx(毫升)phx(毫升)7.107.207.307.407.507.607.7

12、07.807.908.0045.744.743.442.040.338.536.634.532.029.28.108.208.308.408.508.608.708.808.9026.222.919.917.214.712.410.38.57.0三羟甲基氨基甲烷（tris）hoch2 ch2oh choch2 nh2分子量=121.14;0. 1m溶液为12.114克/升。tris溶液可从空气中吸收二氧化碳，使用时注意将瓶盖严。11硼酸硼砂缓冲液（0.2m硼酸根）ph0.05m硼砂（毫升）0.2m硼砂（毫升）ph0.05m硼砂（毫升）0.2m硼酸（毫升）7.47.67.88.01.01.52.

13、03.09.08.58.07.08.28.48.79.03.54.56.08.06.55.54.02.0硼砂na2b4o7h2o,分子量=381.43;0.05m溶液（=0.2m硼酸根）含19.07克/升。硼酸h2bo3,分子量=61.84,0.2m溶液为12.37克/升。硼砂易失去结晶水，必须在带塞的瓶中保存。11硼酸-硼砂缓冲液（0.2m硼酸根）ph0.05m硼砂（毫升）0.2m硼酸（毫升）ph0.05m硼砂（毫升）0.2m硼酸（毫升）7.47.67.88.01.01.52.03.09.08.58.07.08.28.48.79.03.54.56.08.06.55.54.02.0硼砂na2b

14、4o710h2o,分子量=381.43;0.05m溶液（=0.2m硼酸根）含19.07克/升。硼酸h2bo3，分子量=61.84, 0.2m溶液为12.37克/升。硼砂易失去结晶水，必须在带塞的瓶中保存。12甘氨酸氢氧化钠缓冲液（0.05m）x毫升0.2m甘氨酸+y毫升0.2nnaoh加水稀释至200毫升phxyphxy8.68.89.09.29.450505050504.06.08.812.016.89.69.810.010.410.6505050505022.427.232.038.645.5甘氨酸分子量=75.07;0.2m溶液含15.01克/升。13硼砂-氢氧化钠缓冲液（0.05m硼酸根）x毫升0.05m硼砂+y毫升0.2nnaoh加水稀释至200毫升phxyphxy9.39.49.65050506.011.023.09.810.010.150505034.043.046.0硼砂na2b4o710h2o,分子量=381.43;0.05m溶液为19.07克/升。14碳酸钠-碳酸氢钠缓冲液（0.1m）ca2+、mg2+存在时不得使用ph0.1mna2co3(毫升)0.1mn2hco3(毫升)20379.169.409.519.789.9010.1410.2810