【毕业学位论文】SARS 冠状病毒S 蛋白抗原表位分析与定位研究博士论文

) I|Sj q sP : , q EF : M q B q ; s P : 2005M 6 16 SjFh of 3h 2003Mdk?3-?CV.83h?bN3hid#Z#&b hB3h-V$D?CsXTd3hMb1TAUyF#X3hv 2003 f 2003 2003 2003bhVXsVV?Bh 20039/hV?D 2003B hV?bPSE K5VV R 1 100d biT 2h R 1 5000d :F 8iT 1h!Q b T+s#VFTVV#m 2w=) B|VV 2U S 3hb c !RF?!bAad 37CT 1h R F 1 5000d :F 8 37CT 1h RF AA 15s 50l/d 2 490 olb SjVbadBF 37C T 1h !QQ 10s 1:5000 d=F 37CT 1h QQ 10s dAb T V#tVrIVt# S2a1 l*| )8 Mk#Kk(FbFVr) (VrvlM+sVvl 2627Vr(iVr)82b m 33 3b FV Bb#fbf_Tf_TAUVrVF ?$ Bbfb+sMYiOfbQA |5g/x 100.1 b EA 0.1 5mg/ Vr) 4Cib 50 150 )i%ib !A5#45 100BS VF b !A |862li 50 100mlb ! 6c 10 g 5 g 5 g b)iib | g 1 g 5 25 =JJb20C E;ib A8! + 15 g/Lb) 70C F 1 (b 4C i!b j 6c 10 g 5 g 5 g 1 g 7 g jb)sib!-oVb A 20 mg/ 20C E;i-(b 8! + 15 g/Lj) 70C F 1 A( 4C E;iMM55b A 20% (w/v) 2.5 b)ibb )8)8tok4Tb1V/Vv) GB) 510 ! 37C!3 b%)z|joVF 50C!b !|)8 10dbd| 10lFs 200l%)!iT 5b|TzAj (Pjbib 5 37CV!b99)8b SjAFd| dbA 4Cb 2hb f. eAb y ! d =A8bNQ! 5QbVi1d2b g. 100l )8tod 21011)8FdaAi( 60b h. )8b i. f d! 10QiBQ1Bvb j. !dF 100Ai( 15|!AB) F 15l1 , !AA 7Wb k. | 1l!/)8vZE)b l. :)8!A/95|!)8AF7!3* )8%iT 5b|37C 20mlc! 37C! b m. |!AB)5 4C 1000g 10|bM6B5 10,000g 10n. | 80%b6B5F 1/68 / 10Q=bbbQi 4hVb ? o. 4 15)8bb|bb SjA 37C 1h! 3Qb|B)8 d 1012| 100d 3d iT 2h!A! 5QdF 100l 1:5000d F )8F837CT 1h !A! 5Q 20 2 b )8 Vr 3 S $ 1g/d 4C $Vb |B)8 200dPiGQ 21012 21011 2104b| 50l8adF 2 37C T 1hbTF ddQb 37CT 1h!F 5000d :F 37CT 1h! 492D tVrB VrF 2?MY SV 21tb!9H* 59s 5 59a 5b|WI 21t_ Q_b| 8 bv) MkFZp3/K F bFv) s% sVrb -ZE!;t B TBF b SjVR 1 100dF biT 2h R 15000d :F 8iT 1h!Q dAb T )8to )8todrQ!VYV4!Ai7v!Hqo -! -9p)8lqTV 4UbTVB)8b!)8m 4Ub V )8toB 1= 2 3)8 (21010 108108!)8 (105105107! / (n) 10SjV R 1 100d hbfbiT 2h R 15000 d S:F F8 1 5000 d S:F iT 1h!Sj 3hb BSc !RF 100dF hb 37CT 1h!R F 1 5000 d S:F F8 37C T 1h !RFaAA 15s 50l/d 2 405 o b T99 j_(CvlBHm 5 2001 1512 1083 597bpb FykF Mk(TF0bF (Mvlm 5bFp3/bTVuB 1alMsY 1032AG 1035TC blM(llM#TlMb FVrFv) 4h(VrVrvlB 47Vr(d8Ti ,m 5UphUVrHb SjV R 1 100d biT 2h R 1 5000d :F 8iT 1h!Q bfbTHb 1 200d=F 1 5000daS:F 8 dAb _FbVr4fQ cFh r SF b8 5% 4VR 1 100d biT 2h R 1 5000d :F 8iT 1h!Q b _t fbfQ A|2b 50d 100l/d$ 4CV 5% i 3hb BSc !RF 10000d 37CT 1h R F 1 5000d :F 8 37CT 1h RF AA 15s 50l/d 2 490 olb SjV R 1100d biT 2h R 1 5000d :F iT 1h!Q b _t hbfbfQ b8 5% 4V R 1 100d biT 2h R 1 5000d :F 8iT 1h!Q bfbTHb 1 200d=F 1 5000daS:F 8 dAb _t XHF8 fQ A|2b 50d 100l/d$ 4CV 5% i 3hb BSc !RF 10000d 37CT 1h R F 1 5000d :F 8 37CT 1h RF AA 15s 50l/d 2 490 olb _F bVr4fQ Vh Sh SjpVa*pVapVapVapVapVapVaipVaf)VamVaVaVaZVaVVUb Sb1)afpVL3b u+pVa+pVa6+pVL3b Nie=a =#uD3/SELi=3VUb Sj); . X!#FVk . 3 2005 21(2) 211. f) 6 . #Ef5%Y# . SjS 2005 38(1) 176. of 2004, 319 (3): 929(Yy0 6. f) d . xF8 -l%8T . $uD 2004,36(8) 7. , f) d ) fe . v)f . $uD , 2002, 34(5):7SjSpV Te 104 8. , f) . xF8-v) . DZ . 2002, 23(1): 13. , f)dB7v . _ 987p+vf) E SuDS/ , 2002, 32(1):30. f) , 6 . F%Y# . 2jv , 2003, 26(1): 661. fedf) . y _ZEy . Suh , 2003,19(2): 672. fYdIB , u+ . .h2s