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松针不同提取部位体外抗肿瘤作用的实验研究A1A0A2A3A4A5A6A7A3A8A9A10A2A3A11A12A13A2A14A15A16A17A18A19A20A3AA21A22A23A24A25BA21A26A27A28A23A24A29A30A31450008A32A33A34A35A36A37A38A39A40A41A42A43A44A45A46A47A48A49A50A51A52A53A54A55A56A57A58A59A55A60A61A62A63A64A45A65A66A40A41A67A44A68A69A70A46A47A71A72A73A74A75A76A77A78A79A80A81A80A82A79A83A84A67A85A86A44A45A46A47A73A87A88A89A90A91A92A93A94A95A96A97A65A98MTTA65A99A100A101A102A44A45A46A47A103A104A105A106A107A108A109SMMC7721A79HEPG2A73A104A110A111A106A107A108A109MCF7A73A104A112A113A111A106A107A108A109PC3A107A108A114A115A48A116A117A54A55A56A59A55A118A119A107A108A120A71A121A107A108A122A123A71A124A73A125A38A40A41A51A52A53A54A55A126A127A56A128A129A76A77A78A46A47A103A52A53A107A108A48A116A117A54A55A130A131A73A132A133A134A63A135A136A137A138A139A140A141A142A143A144A63A135A145A146A147A148A80A81A80A82A46A47A48A54A55A149A150A148A83A84A67A144A86A46A47A48A116A117A54A55A42A151A152A56SMMC7721A107A108A15306MGML1A7908MGML1A76A77A78A44A45A46A47A154A12748HA155A48A156A157A158A71A159A71A160A161187A79214A73A162A103A163A107A108A114A115A48A116A117A164A66A107A108A122A123A165A166A132G2MA123A56A128A167A40A41A44A45A68A132A49A50A168A169A51A52A53A54A55A73A76A77A78A46A47A139A140A141A130A131A48A116A117A54A55A73A170A49A50A116A117SMMC7721A107A108A114A115A48A126A117A171A172A173A40A41A44A45A68A48A107A108A174A54A55A79A175A176A107A108A156A157A144A116A117A107A108A71A177A169A178A56A179A180A181A35A40A41A148A52A53A107A108A109A148MTTA65A148A118A119A107A108A120EXPERIMENTALSTUDYONANTITUMOREFFECTOFDIFFERENTFRACTIONSOFPINENEEDLESINVITROZHOUWEIA,ZHENGXIAOKEB,WANGXIAOLANA,FENGWEISHENGAHENANUNIVERSITYOFTRADITIONALCHINESEMEDICINE,ASCHOOLOFPHARMACYBBASICMEDICALCOLLEGE,ZHENGZHOU450008ABSTRACTOBJECTIVETOSTUDYTHEANTITUMOREFFECTOFDIFFERENTFRACTIONSOFPINENEEDLESINVITROMETHODSPINENEEDLESWEREEXTRACTEDANDSEPARATEDBYTHETECHNIQUEOFSOLVENTREFININGMTTASSAYWASUSEDTOOBSERVETHEINHIBITIONOFEXTRACTSONTHEPROLIFERATIONOFHUMANHEPATOCELLULARCARCINOMASMMC7721,HEPG2,BREASTCARCINOMAMCF7ANDPROSTATICCARCINOMAPC3APOPTOSISRATESANDCELLCYCLEDISTRIBUTIONWEREDETECTEDBYFLOWCYTOMETRYANALYSISRESULTSPETROLEUMETHEREXTRACTFROMPINENEEDLESPOSSESSESTHEROLEOFANTITUMOREFFECTINCELLCULTURETHEINHIBITORYEFFECTONTHEPROLIFERATIONOFTUMORCELLSHASREMARKABLETIMEDEPENDENCEANDDOSEDEPENDENCEPETROLEUMETHEREXTRACTINDUCESSMMC7721APOPTOSISANDARRESTSING2MPHASEAPOPTOTICPERCENTAGEOFPETROLEUMETHEREXTRACT06MGML1AND08MGML1ONSMMC7721IS187AND214IN48HOURSCONCLUSIONPINENEEDLESFRACTIONSHAVETHEANTITUMOREFFECTINVITROANDTHEPETROLEUMETHEREXTRACTPOSSESSESTHEROLEOFANTITUMOREFFECTANTICANCERMECHANISMOFPETROLEUMETHEREXTRACTONSMMC7721MAYBEDIRECTCYTOTOXICEFFECT,INDUCINGCELLAPOPTOSISANDINHIBITINGCELLSEGMENTATIONKEYWORDSPINENEEDLESCARCINOMACELLLINESMTTFLOWCYTOMETRYANALYSISA182A183A184PINENEEDLESA185A186A182A187A184PINACEAEA185A182A188A184PINUSA185A189A190A191A192A182A184PINUSMASSONIANALAMBA193A194A195A196A197A198A199A200A201A202A203A204A205A204A206A207A201A208A209A210A211A212A213A214A215A216A217A218A219A220A221A222A195A223A224A225A226A227A204A228A229A230A231A232A204A233A234A235A236A237A238A239A240A204A241A242A243A244A245A246A194A247A248A212A249A221A250A251A252A253A254A255A87A227A0A1A2A3A4A5A245A246A217A218A201A219A59A6A7A8A216A9A10A11A12A13A204A108A14A13A227A15A16A198A17A18A19A200A201A111A255A10A11A12A13A227A15A16A3A4A5A97A214A233A20A21A115A201A116A22A23A13A1A2A248A233A234A216A24A235A236A237A238A25A26A201A111A27A28A29A30A31A32A33A34A206A212A130A253A224A35A222A195A225A235A236A245A246A194A221A250A217A218A83A36A37A216A201A38A39A201A249A136A137A40MTTA139A201A140A97A214A112A41A194A143A209A20A237A238A49SMMC7721A204HEPG2A201A143A42A43A20A237A238A49MCF7A201A143A253A44A43A20A237A238A49PC3A38A45A46A201A150A47A48A50A51A52A53A54A55A56A57A64A58A60A154A61A62A63A65A66A154A61A67A68A69A70A71A72A73A74A62A75A76A77A78A79A80A81A54A64A58A60A163A82A95A84A85A154A61A67A68A165A86A88A89A90A91A92A93A94A96A98A9911A58A60A100A101A102A167A103A104A105A100A101A102SMMC7721A106HEPG2A62A103A107A109A105A100A101A102MCF7A62A103A110A113A109A105A100A101A102PC3A92A114A117A88A89A118A119A120A121A122A123A12410A125A126A127A128A55RPMI1640A121A122A90A141A129A124A171A131A132100U/MLA62A176A131A132100U/MLA133A62A17737A178A1065CO2A179A134A135A138A121A122A142A141A121A122A6223DA183A1441A185A62A53A65A153A145A187A146A100A101A61A123A88A89A9212A147A54A188A189A148A167A149A151A50A50A51A152A155A156A157A158A126A159A160A161RPMI1640A121A122A90A85A193A145A125A126A127A128FCSA62GIBCOA194A162A161A196A164A90A166A168A129DMSOA85A200A164A90A201A169A203A204MTTA62AMRESCOA194A162A161A205A170A172A173TRYPSINA62GIBCOA194A162A161A209A210A174A175A180A1295FUA133A62A214A181A182A184A181A186A147A190A191A192A194A162A62A221A195060703A161A223A197A198A180A129PIA133A85RNASEAA62SIGMAA194A162A161A66A199A189A148A202A75A206A207A84A208A211A9213A230A212A232A213A167A234A215A100A101A230A62COULTEREPICSXLA161ELIEA216A100A101A121A122A142A62A236A206REVCOA194A162A161SWCJIFDA237A217A239A154A218A62A241A217A219A220A244A222A194A162A224A225A161KDC160HRA247A226A227A228A229A231A67A62A233A235A238A193A240A242A191A192A194A162A14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