标准解读
《GB/T 44708-2024 化学品 蚯蚓繁殖毒性试验》是一项国家标准，旨在提供一种评估化学品对蚯蚓繁殖能力影响的方法。该标准适用于固体、液体和半固体形式的化学品，通过实验室条件下控制的实验来测定这些物质对蚯蚓繁殖活动的影响程度。
根据此标准，测试采用特定种类的蚯蚓作为实验对象，在规定的人工土壤中进行。试验设计要求使用至少两个不同浓度水平的待测物以及一个对照组来进行比较研究。整个过程需要持续几周到几个月不等，具体时间取决于所使用的蚯蚓种类及其生命周期特性。期间需定期记录成年蚯蚓数量变化情况及后代幼虫的数量，以此为基础计算出一系列指标如EC50（引起50%效应浓度）等，用以量化化学品对蚯蚓繁殖功能的潜在危害。
如需获取更多详尽信息，请直接参考下方经官方授权发布的权威标准文档。
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文档简介
ICS
13.300
CCS
A80
中华人民共和国国家标准
GB/T44708—2024
化学品蚯蚓繁殖毒性试验
Chemicals—Earthwormreproductiontoxicitytest
2024-10-26发布2025-05-01实施
国家市场监督管理总局发布
国家标准化管理委员会
GB/T44708—2024
目次
前言
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Ⅲ
1
范围
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1
2
规范性引用文件
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1
3
术语和定义、缩略语
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1
4
受试物信息
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2
5
试验原理
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2
6
参比物
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2
7
试验系统
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3
7.1
仪器设备
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3
7.2
试验生物
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3
7.3
试验土壤
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3
8
试验程序
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4
8.1
受试物添加方式
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4
8.2
暴露条件
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4
8.3
预试验
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5
8.4
正式试验
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5
8.5
限度试验
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6
9
质量保证与质量控制
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6
10
数据与报告
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6
10.1
数据处理
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6
10.2
统计分析
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7
10.3
试验报告
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7
附录A（资料性）赤子爱胜蚓/安德爱胜蚓的驯养
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9
附录B（资料性）土壤饱和持水量测定方法
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10
附录C（资料性）蚓茧孵出的幼蚓计数方法
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11
附录D（资料性）计算NOEC的数据统计流程示意图
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12
参考文献
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13
Ⅰ
GB/T44708—2024
前言
本文件按照GB/T1.1—2020《标准化工作导则
第1部分：标准化文件的结构和起草规则》的规
定起草。
请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。
本文件由全国危险化学品管理标准化技术委员会（SAC/TC251）提出并归口。
本文件起草单位：生态环境部南京环境科学研究所、生态环境部固体废物与化学品管理技术中心、
上海市检测中心、中检科健（天津）检验检测有限责任公司、宁波海关技术中心、沈阳沈化院测试技术
有限公司、浙江省农业科学院、苏州华测安评技术服务有限公司。
本文件主要起草人：郭敏、石利利、王蕾、廖建华、刘纯新、吴文铸、葛海虹、周丽丽、朱昱璇、
杨希晨、袁野、刘汉伟、石凌锟、汤涛、皮天星、王雷、张雅静、李贝贝。
Ⅲ
GB/T44708—2024
化学品蚯蚓繁殖毒性试验
1范围
本文件规定了化学品蚯蚓繁殖毒性试验的受试物信息、试验原理、参比物，试验系统、试验程序、
质量保证与质量控制、数据与报告的要求。
本文件适用于测试与评价化学品对蚯蚓繁殖的影响。
本文件不适用于易挥发的化学品，如空气﹘土壤分配系数大于1的化学品，或蒸气压（25℃）大于
300Pa的化学品。
2规范性引用文件
下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。其中，注日期的引用文
件，仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用
于本文件。
GB/T21801化学品快速生物降解性呼吸计量法试验
GB/T21802化学品快速生物降解性改进的MITI试验（Ⅰ）
GB/T21803化学品快速生物降解性DOC消减试验
GB/T21809化学品蚯蚓急性毒性试验
GB/T21831化学品快速生物降解性：密闭瓶法试验
GB/T21845化学品水溶解度试验
GB/T21852化学品分配系数（正辛醇﹘水）高效液相色谱法试验
GB/T21853化学品分配系数（正辛醇﹘水）摇瓶法试验
GB/T21855化学品与pH有关的水解作用试验
GB/T21856化学品快速生物降解性二氧化碳产生试验
GB/T21857化学品快速生物降解性改进的OECD筛选试验
GB/T22228工业用化学品固体及液体的蒸气压在10-1Pa至105Pa范围内的测定静态法
GB/T22229工业用化学品固体及液体的蒸气压在10-3Pa至1Pa范围内的测定蒸气压平衡法
GB/T27850化学品快速生物降解性通则
3术语和定义、缩略语
下列术语和定义适用于本文件。
3.1效应浓度effectconcentration；ECx
在给定测试周期内，与对照组相比，
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