《烟草品种鉴定SNP标记法》

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前言

本文件按照GB/T1.1—2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的规定起

草。

本文件由国家烟草专卖局提出。

本文件由全国烟草标准化技术委员会农业分技术委员会（SAC/TC144/SC2）归口。

本文件起草单位：

本文件主要起草人：

I

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烟草品种鉴定SNP标记法

1范围

本文件规定了利用单核苷酸多态性(singlenucleotidepolymorphism,SNP)标记进行烟草(Nicotiana

tabacumL.)品种鉴定的操作程序、数据记录与统计、判定方法。

本文件适用于烟草品种的SNP指纹数据采集及品种鉴定。

2规范性引用文件

下列文件对于本文件的应用是必不可少的。凡是注日期的引用文件，仅注日期的版本适用于本文件。

凡是不注日期的引用文件，其最新版本（包括所有的修改单）适用于本文件。

GB/T21138烟草种子

NY/T2594植物品种鉴定DNA指纹方法总则

YC/T369植物新品种特异性、一致性和稳定性测试指南烤烟

3术语和定义

本文件没有需要界定的术语和定义。

4原理

不同烟草品种的基因组DNA存在核苷酸序列差异，其中单个核苷酸(A、C、G、T)的替换引起的这

种多态性差异称之为SNP。SNP具有分布密度高、通量高、数据整合易等特点，不同品种的SNP位点

的基因型可通过扩增及芯片扫描等技术进行检测，从而能够区分不同品种。

5试剂

5.1DNA提取试剂盒。

5.2SNP芯片试剂盒。

6仪器设备

6.1样品粉碎仪或研磨机。

6.2恒温孵育器或水浴锅。

6.3涡旋振荡器。

6.4PCR扩增仪。

6.5离心机。

6.6电泳仪。

6.7凝胶成像仪。

6.8核酸浓度测定仪。

6.9杂交炉。

6.10SNP芯片分型仪。

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7标记

SNP标记相关信息见附录A。

8操作程序

8.1样品准备

8.1.1从烟草品种材料中抽取的个体的数量应满足NY/T2594要求。

8.1.2样品中个体的类型宜为幼嫩且新鲜的叶，也可为种子。试验样品为种子时，其质量应符合GB/T

21138中对烟草种子纯度的要求。

8.2DNA提取与检测

提取与纯化的DNA溶液在260nm与230nm处的吸光度值的比值介于1.5与2.0之间；在260nm

与280nm处的吸光度值的比值介于1.8与2.0之间；DNA电泳主带明显，无明显降解和RNA残留；DNA

浓度>10ng/μL。

注：能够达到相应质量要求的DNA提取方法都适用于本标准。

8.3SNP指纹数据采集

8.3.1基因组DNA变性及扩增。取200ng样品基因组DNA变性后，分别加入SNP芯片试剂盒中扩增

混合液、扩增聚合酶，置于37℃杂交炉中恒温孵育24h进行全基因组扩增，65℃孵育20min终止扩增

反应。

8.3.2DNA片段化及沉淀。分别加入片段化缓冲液、片段化稀释液、片段化酶，置于37℃杂交炉中恒

温孵育30min进行片段化反应，随后加入终止液终止反应。分别加入沉淀液，混匀后再加入异丙醇，封

膜后转入-20℃冰箱，沉淀DNA。

8.3.3DNA干燥和重悬。将沉淀离心倒掉上清液置于37℃杂交炉中20min进行干燥。随后加入重悬缓

冲液，重悬溶解DNA。

8.3.4杂交。分别加入杂交缓冲液、杂交液，95℃变性10min后立即置于48℃的金属保温板中。吸取

样本加入芯片加样区，置于SNP芯片分型仪中杂交24h。

8.3.5芯片扫描。分别加入染色液和缓冲液至对应位置。运行SNP芯片分型仪，完成连接、染色、洗

涤和扫描工作。进行数据分析处理，同时保存原始文件。

注：以上操作程序仅为推荐方法，其他能达到同样位点分型效果的方法也可采用。

9数据记录

根据SNP标记位点的分型效果，剔除或留用该位点的数据。数据记录为X/X或X/Y，其中X、Y

为该位点的单核苷酸，如A、T、C、G，按此顺序排列。无效数据记录为-/-。

一个样品的有效数据所占比例若<95％，不予采用。

10结果分析

10.1结果计算

用附录A中标记检测，获得待测样品在各标记位点的DNA指纹数据，利用这些数据进行品种间的遗

传相似度比较。遗传相似度按式（1）计算

………………（1）

n

=×100%

2

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式中：

——待测品种与对照品种的遗传相似度，%；

——待测品种与对照品种中均检出的但基因型无差异的标记位点的数目；

ij——待测品种与对照品种中均检出的标记位点的数目。

ij

10.2结果判定

当样品间遗传相似度≤96％，则判定为“不同品种”；当样品间遗传相似度＞96％，判定为“近似

品种或疑同品种”。

对于“近似品种或疑同品种”的样品，可按YC/T369的规定进行田间种植进一步鉴定。

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附录A

（规范性）

SNP标记

SNP标记见表A.1。

表A.1SNP标记

序号染色体位置正负链序列

S11602184-ACTAATTAAAAAATAACTAAGGTGCAAAGTAAATC[T/C]TAGTGAAACATAATAATATCTGACAACGAAATATG

S212328654+TTTGAACTCTGATGGGCTCGACTAAATAACAATCA[C/T]GGTGAATTTAGTGGGTAGTTAATACAAGCCTCTGT

S313233714-ATGCACGTTTGCCTAAGACCTTGGAATCAGCTGTA[C/T]TACTTGATGCTCAATATATTGCTTGTGGAAGCACA

S416045225-AAGAACCATCACAATTGTGAAGTCATCCACATTTC[T/C]GTTGCCTTCGCAAATTGCGAAGGTAAGTTTGCAAA

S517289665+AGCATTCTAAGTACAAAGCTATTAACATTTCAGCA[A/G]CCAAAATCAACATTTCAGCAGCAAAATCAGCATTT

S618165810+GGAAGCCAAGTGCAGCACCGTTACCAATACCAAAA[G/A]ACTTTAAGATGCCCGATATTCCTAAATATGATGGG

S719763770-CAAAATCTACAATAAGTATAAACGCTAACTTTCAA[T/C]AGGATAAAACATGCCAAGAGACTGGAACAAACCCT

S8111443707-ATGAAATATAAGTCAAAGTACCAATATGGAATAAA[C/G]ATAAAAATAATTTAGTAAAACACGAACAAGAAATA

S9112281249-TCTAGAGAAATGGTGGAGAAGTTGAGATGTAGAAC[C/T]GAGAGGCAAGGAGCACGTTTTATCTCGTTTAATCC

S10113070704-CAGATGGAATTAATCATAAGCTTAACTCAAGGAAT[C/A]AGATAAATCAAGATCTCACTACATATTCATCAAGT

S11114230693+TACTAAAGCCAGATGGACCAGAACCGACAAAAGAA[C/G]CTCGACAGCAGCACGCCAATTCAGAAGCGCATCAC

S12115640723-TGGAAATCCCTTAATTTTGATACCACTGCTCTAAG[C/A]TGCGATAATATCAATTGGCTAGATGCCATCAAACA

TTCTATATATATGGGACCTTTTTTCTTAGAAAACC[C/G]TAATAGTACAAATATTAGGAATATTCACTAGAATA

TGGTACACCGCCGATATCTTGTGAGATAACGTCAA[T/G]GTGCTTTGTCACGAGGAGGGACTTTGCGTTAGAGA

AACGTAGTCTGCTGCCTCCGTGAGAGAAAGCCCTT[C/T]ATACTCCATTAGAGCAGCCACATCTCTCGCTACAG

S16122439721-ATTTTTTAGCAAAGTTGATAGGGTAACATGTGACT[T/C]AGCACTATCAACTTAACGTCAGAAAGATCATTTTC

TTTACGCTGAATCCGGAGGAGGATTTCATCTCAAG[G/A]GATGTGATGTAAGGAGCCTACTCTGATACACGTAT

TCCGCAGATGCGAAGTTTGAAGATTTGGGCATTTC[T/C]GTAAAAGTGGACTAAGGGCCGCAGAAGCGCCTCCG

S19127017134-TCACAATAGCATTGTATGTACTGCCTAAGAAAGTG[G/C]CACCTACCGTGGCTAAAAAACGGGAAGTAGAAATC

S20128267949+TCAATACATAGATATCTGACATGAAGTTTAAGTGC[C/A]AAGTTGAACCAGCGATCCACATGAGAAGCCAATTC

S21128601351-TTTAAATGTCTTTTACGATTGGTTAAATATCTCTG[A/T]TTCTCTCTAAAAAACATTGAAAACACCAAATCTGA

S22129309274-AAGTATGATGGGACTACAAATCATCAGGATAATAT[T/C]ACCGCCTATATAACGGCAGTGAAGGGAAACAATTT

S23130628325-TGAAGTTCAGTCCATAGAAAACACTAAATAAAACA[C/T]GCGCAAGTAACACCGAGGGACGTACGGTCCGATCT

S24132415288+AAGTGCATTTATTGCAAAGATATATGACCGGTACA[G/A]CCATTTAATGCCACGGTACGGGCCTGAACTAGCCT

S25133067181-GGAGCCGCACCACAGATCATCGTGTAACCTCCTTT[C/T]TGCAGCCATAGCGGCAGCCTGAATTGGGGTGAGTG

S26133430580-TACAAAGAGATACAGACCTGACAGATAGACAGAGG[A/C]ACACGGTGAGGATATAGGAGATACACTGAGAGGGA

S27134152695+TCCACGTAATCCATCATTTTGTTGATATGAACTGT[T/A]GTCAAATGTATCAAATTGTTGTCCTTTAGGAATGC

S28135344571+GGACGAGAAATCATAGAATGCTTCGACAATCTCTG[T/C]ATCCCCAGTCCAGGTAGATTGTTGATGTCTATCTC

S29136324277+CTTTTGATTGATTCAAAACATGAACGCAAGCACTC[G/A]TATAAGTATGCAAGCGAGGGACCAACGCAGCCAAA

S30138995108+TATGTTCTTCTGTATTTTGAGACTCTAAGTTCGAG[A/G]ATGAGAGTGAGCGATTGAACTCGTGCAGTTAAGGC

S31141173300-TCTAACATGTGGAACCCACCTCGCTAACTTGAAGC[T/C]ATCCCACATGCCTGGCAACAAGCTTCTGTAATTGC

S32143535978+CATCCGATAAATTTGGAAGGAAGATCATGACGATC[A/C]TGCGAAAGGATAACATGCAGAAATCACAATATTGT

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表A.1（续）

序号染色体位置正负链序列

S33145181817+ATGTTACTCTCCATGTAAGCCAAGTTCTCCATCTT[C/T]AATGTGCTAGAACATAACTCCTTGAAAAATAAACT

S34146592072-GTGTGGATAAATAATGTCTAATAATTATTGGCATC[C/T]GATTGGTGGTCACTCTTTATAAATAGAGATCAACA

S35148566755-AAATTTCAATGCCACGCAGGATCGCTGGAACTTAA[C/T]GAAAACAGGGTATATTTGAACTAATATTATAACGA

S36150187135+GGAGCCTATCACTCAAGATGACAAGATTAATATTG[C/T]TTAACATGGGGAGTCCTACAAACCACACAAAGGAC

S37152525648+AGTATAGCCTTTTATCCACATTTGATGGGCAGGCA[G/A]AGCAGACCATTCAGATAATTGAAGATATGTTGCAT

S38153749378+AGTAAAAGCCAAAGAACATGTCCCATATAATATTC[T/C]CCATGCACATGCATGGCCATGCAAAGACAGACTTT

S39155047840-AAGGAGGAGAAGCTCATTGTTTATGAATATGTTCC[T/C]TGTGGTGACTTGTTCGAGCTTTTACATGGTTGGTT

S40155853411+AGGTTGAAAAACATATTTGAGTCCAAAAATTGAGG[C/T]GGGTCTAAATCTGGGGCGGGTAGAAACCAATCTGG

S41157062163-TGGCTAATACCTGGTACAAGCCTACAACAAAGTAG[C/A]ATATGCAAAACATATGGTGGAAGCTAAAAGAAATG

S42158561969-TAGTAAGTTGCATATGAGAGTTTCAAGACTATGCC[T/C]TGTTTTGTGCATTGTATATACTCTCATTGTTTTTG

S43159330866+GCAATTGGAAGATATAAAAGCAGCCACTACTTGAT[G/A]TTATTTTTGGCTTTGCTTGGTTCCTGCATGATAAT

S44160309861-AGGAGGTAGTAGGGTTTTAATATTCTGTTGATTGA[C/T]GTCTAGGACGTTCAGAGTGAGGGAGTGGTTTGCTA

S45162141714-TGCTACCTTGATTAACATGGTATTTGAGCGCGTTA[G/A]TTGTAATAAAATTTATGAGAATTTGTTATGATTCG

S46164793038+GAAAAGATCGGGAGAGAAGGTAAGTAACATACTAT[C/T]ATGGTCCTGAGCAAGTCACTCGGGACGCTCTGATC

S47166883544+TTTGATAGTGCAATGGAATGTAGAGGAGGGAAGGA[G/T]AAAGGGAAAAGCCATCCCCAGTTAGGAGTATCACA

S48167568043+GCTAAATATTACAAGTATAGCCACCGAACGTAATG[A/C]AGTCTTTAATTGTTGAAATTGCGCATATAGCCGCT

S49168802740+AGAAATAGTATGAAAAATTGTAATTGATGCCAAGG[C/A]ATAGTTTCGAAAATACTTTGGGGAAAAATTGGGTA

S50170278499+TACCATTTGCATGTATTACGGTCTTTAAATTGGAA[A/C]TGCCTTTAGAACTATTGCTAGGGCAATACTTACAT

S51172063965+AGGTACTCTCCCTTCGAATCTATTTATCTCTCTTC[A/C]TTTGCATTTTACTTCACACTTTTCCCCTTTATTTT

S52173496361+GATTGCTTCCATCTTCTATTCCATATCTCAACTCG[G/A]AATCTTCTTCAACATCAACGATTCGAGCTATATAG

S53175014014+GTATTGCTTTCATGTTACAGAAATGTTTAAAACGA[G/A]GGTCGGCCTACGTACGTCTCATTCAAATCGAATGT

S54177145399-TATTTAGGTTTTGTTGGTGAACTAAATGGTTGTGA[G/T]ATGTGTGTAGGGATGTTTGATGCAGTGCAGGGCTA

S55178231796-AACGAATTACAACTGATTCATGGAACAAAAAAAAC[C/G]AAAAACAAGAAAAAGGAGGTTCCAGACTGCTATTA

S56179766998-CACCTAAGCTTAATTTGATAAAGTGAAAGAGAACA[G/A]GAATGAAATTAGTCTTGGGGTGAAAAAGCTATGCA

S57181769077-TGATTGATTACATAATTCATATAAGAGGCCTGAGT[T/C]AGGCCTGCCTGCTGGTTGTAGTTAATAGCACAGAT

S58183345252-TGTCCTCTGCCTTCTCCTCTTGGTTTTTTTTCATA[C/A]GGGAGATTGAGGAGGAATGTCTTATTAATTGTAAA

S59184037640-TGAAACCTGGTACATGATGAGTATTATTATTCTCA[T/C]TGGCCATCTCAAAGATGCAGTCATTGCAGTTGGAT

S60184847791+GAGGCCAATACATCTATCCTGGCCGGAAGGGCATA[G/A]AATTTGGTTGTAGCGTCGGCTGGCTGGCATCCACC

S61185992956+TAAATCCAGGTCTTATCAAGATTGAATAAAAGTGC[G/A]GACCGCACATGGAATTGTACGGCCGTAGAACCTCC

S62188994892-GTGGTAAGAACTTTAGGGCGGTTTGAGGTGTCGCC[A/G]TCGACCGGTGGGGATTTGGCATTTAAGGGCGGCTA

S63190397936-GTTGATGAGAAAAACAATAAATGGGAAAAGTTGAA[T/C]GGATGTTGACTTATGTGTAAACGACCTCGTATTTG

S64191052040+TTACTAAAGTGACATAATAGGAATTGTTGGGAAGC[G/C]TGTCAAGATAATAAAAGGAAAGGATATTTAAAAGA

S65192438478+TTAATCCTTACTCTATGGTATTTTCCGTGCTGGCC[A/G]CTCTATACTTCATTGACTTCTTAGCAACTGAGAAG

S66193909358+GGACAAGGCTGCATTAATTGAGTTTCATTCTTTTC[A/T]TTGACTTCTCCAAACATTGGCAGCATGCGTGGAAG

S67195233292-GGTGGTAGGGAGCCCAAAGAAAACAACAATGCTTA[T/C]ACAAGGAGGAACGCAACACAATGAGGCAATTGATG

S68197197544-CCAATTCAGATAATTCGCTCAATTGATGTAGCCCA[G/A]TACAACTACCTTAACTACAGAAATCCATATTTCAG

S691100539940+AAGGATTTGTTAGACCTTGGAGCATTGTTGAATGA[C/T]GGGGACAAAATAGATATATTTATCTCCAATGACTC

S701101346189-GGTCCAATTTTTCGAAGGTGCCTAAAGGTATGACC[A/G]AAGTCTCGGAGTCAGTGATGAAAGAGGCCGATCTG

S711102883078-AAGGAAATGGCCAAGCTACTTAGTAAAAGTTGCCG[C/T]CGAGAACTTTTGAGTGATCGGGCTCAAGTTCAGCT

S721104144693+ATCTAGTCATATACTCACAACTACTACATTTCATC[G/A]CGGTGTAGTCGGTAATCCTTATGACTCTCTCTCTT

S731105780661-TCATTGTGATGGATGCTTTAACAATTGGAATGTTC[C/T]CGCTTCCGGCTTTGGCAAGGTTCGCTACACACCCG

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表A.1（续）

序号染色体位置正负链序列

S741107195036-TCACCTATAGCCTTCTACTAACAAATTCTAAAATC[A/G]GGCTTTTACCATATCAATTTTCTGTCATAGTGTTT

S751108760612-CTTCTACCCTTTCCTCTAGTTCTCTTCCTGAATTT[C/T]GCAAGGTATCTTCTGTTGACATACACATGCATGAG

S761110131956+TATAACAAGCCAATGAAGTCATGTTTAATATCTCA[A/T]TGCGGCATGCAGCCTGATCCCATACTATCACTCAA

S771111837216+AAATGTTAATGCCTTTGGGATATGATATAGAAGTT[A/C]ACTCTGGTCTGAACTAAATTGACTTTTCAATTCTT

S781113301177-ATTGCCAATTATAAATTTTTGACGTGTTTGATTAA[G/C]CAATTAACTAATAATGCAAAAGTTGGTTACTTTTG

S791114014037+AAGATCCTCAGGTAACTGATAGAGCAAAATCCACA[A/C]AAACATACAAATAAACTTCACCCCATCCCATACTA

S801116192794+TTTCAACAATTAAACATGTAAATGATAGATAATAA[T/G]AAATACAAATACATAAAGGTTCGCCCCTCGGGCTC

S811118873109-AGGAAATAAATGTTACATCTTTGAAGTAGGAAGAT[T/C]AAAGCATCTTATTGAATTGGATTTTTTAGAATTTC

S821120312277-TCATTGAGGTCTTATAAATCTGGGCATGCATTGTT[T/G]AGGTCTTACATATTTAATACGGCCTCACTGAGGTC

S831121409730-AACTGTTACCACATGTATGTCATCAGAAGGAATGG[T/A]TGTTATTCGTGCCTTGGTCTATGTTATGTGTTTCT

S841122295945+AATAGTGAAGCTGATGATATTGCTATCTTAGGGTC[A/G]TCGGTTGATGACGACGAGTTCATCTGGGGAACAGT

S851123991541-CCTGTGCATCCCTGATTTCCCTCGATAAGTACGAA[G/T]GGGCCCAAATTAAAGCCACCTAGGGCATTAATTTC

S861125821705-TGTAGATTTTTTTACTATACAAGTCCCTTTTGGTT[C/T]AGTTTAGTATTTATTGATTGTAAAGGCTTGTGGTT

S871127118852-TTAAAACAGTAGGAATATGCAAAAGGTCAAGATAA[C/T]AATAACTACGCAGCCCCAACAATCTTGTATCACAA

S881128436840-AGATTAACAATTACTCATTCTTGCAGTTAGTATTG[G/T]GCCCTTTAAAACTAATTTTCTTAATTATGGGCCTT

S891130002494+TGCCTCCTCTCACATTAAGTTGAGAATTTCACGTA[C/A]AGATCTGAGTGGTTCTGCAATTTCTTTGTCTAATA

S901131232063-GTTGAGGTGTTTTCATCAATTGTAGCCTCTATGAC[T/C]AGACTGACATAGAAGGGTGCTCCATTCCAATGGAC

S911133208051-GTGCTGGCCACTAGCAGCTTGTTTGTGGCGCGCCA[C/G]AGAGACCTACCAGCCATTTTTTTTTGTGGGGCCCC

S921134166462+TTTAAACTCAAAGATGGTCCCATACCAACCTCCAC[G/A]CTGACTAGCCAAAATAGGGCAACATCATCTTGCCA

S931135970979+AGGGAAGAGATTGAAAGACATGGTTCAACGAGAGA[T/C]GGGCATAACAATGAGTTTCTTGATTGGTTTCGTGC

S941137134810-CTAACACATGCTTATATCCCATTCAACATTCAAAA[G/T]CTTAGGAGGTACCTGGTTACAAAGGACAAAATGAA

S951139338397+ACAGAAGAGGGAATATGACCTTGTCTGAAGATGTG[A/C]ACTCAAGCTCAAACCACTTCAATCCAGCCTCTGCG

S961140362070-CGAGTGATATGAGGCCACGTGCCCAATATACTTTA[T/G]ATACCACGTGGTGGCTTGAGTGATGATGCCACGAG

S971142085986+TTGAGCTTTCAAGTCTCTAAAATGGTAGACAATGA[G/A]CCAAAATTATGAAACAGGGCTATTTATAGAAGTTT

S981143602499+GTATAATTCATCATTCCACCCACCAATGACATCTC[T/C]GGACCGAAAAATGAAATGATTAGACAAGAAAACTC

S991144649667+AAATCCAATGGTACAGTAGTTTATATGATATCCTC[T/A]ACCTCAAGAATTGCTTCTATACTTTTGGGGTTTCC

S1001145372113+ACTCTTACTCTCCTAAAACATATGCAATTGGGCCA[T/G]TACACTTGCACTTAAAGGCTAAACTTGCTTCAAAA

S1011146647316+ATTCAAAGAGCGCTATACATCAAAACTTTCAACCC[C/A]CCGGGCTAGCCATTTCCTATACAAAGGGGTTAGGA

S1021148267359-AGAAGAGCCAGTAGATGATAAGGAAGTCATGAACA[G/T]TTGGTTTGAGGGTGGAATTTCTGAGTTTCGTAATA

S1031149395864-TACTTTTGGATACTGGTTGATGAAGTATTTTTAGG[C/T]AGTGTTGGGTTCGATTTTTTCTTTGTTGGTTTTGA

S1041150707418-CTAGGTGTTATAATTACTTTATGGTCCAAGTTCAT[T/G]TCCAATAAGAGGTGGATTTGATCCCAAGAAATATC

S1051152300045-TGCATGAACATCATAATAATCATCATAGGAAATAC[G/A]AATACTGGGATGAAACAATAGAATACCCTTTGGTA

S1061153630261-CAGAAGCAGATGGTTCTCAAGTGCATACTGAACCA[G/A]AAGCAGGGGAATCACAATTCGAATAAGGAACTCGA

S1071155106848+ATTCACAATCTTATGCCTTTGATTATCTCGTACGT[C/T]GTTCATTTTTACCTTACTTACCTTAAAATCTCGCA

S1081156907427+TCAACTATAGCACACAAACAATTCCTTTATCCACT[A/C]TTCACCAAAGAAAATCAAATACACATACTATACTA

S1091158181435+TACACATGGGATGATCTAGTCCAAGATTCGCTTGC[T/C]ATTTTTAGTATAACCTCAAAATTGTTCCGGATCGT

S1101160198103-CTTTTACCGCGTTGATTAAAGCATATTTGTTATCC[A/T]CATCATTTGGATTGATCGCCATATTTGGGCTTCGT

S1111161423257+AACATACATAAATTTCTTAGCACTGTACTAGCATA[C/T]ACAATATGCACAGAAAACCAAACTAAGCATGAATC

S1121162742584+AAAGAGATCTCTAACACTTGTTGAGTTTCCTTCAG[A/G]CGTTGTATGGTCGTTGCCTCGTTAAAAACCTTGTC

S1131164406536-ATAAAAAGAGAGACCTAGAAACACAATAGGATCGG[A/C]CGCTCTTACACCATAACAAGCACCCCAAAAAAGAA

S1141165644941+AGGTCTAATATGTGTTGTAATGTTTTCCTGGATTG[T/A]GTAAACTAGAAGGTCCTTGGTTAATGAAGTGTCTA

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表A.1（续）

序号染色体位置正负链序列

S1151167801514-ATACTTGTATAGGATAAAATTGGGTTATATAAAAT[G/A]ATCGAATAGTAGAGTGACACGTGAAACCGAAGACA

S1161168621445+AAAAAAGAATTATACAAGAGTAAGTCTGCTTGAAA[A/G]GACAAGAACGAAATTCTCTTAGAAAACAACTGACG

S1171170134953-GTCACTTCAGCTAATCGAAAGCAAGCAATCCATTC[G/A]TGTCAAAACCATAGAATCACAGGTGTTAGAGTATT

S1181171951120+TCGAAGTCTCTAGAGTACCTTTAAGGCGACGTCAA[A/C]CGCGCCAGAATTGCTTGCGAGGAGCTGAGGGCTCA

S1191173445193-ATAGAGATTCAATTAAGGCAAGGGTCCAAATCATA[C/A]CAAATAAGGAGTCAGTAAGAATCCTAAAATTATAC

S1201175031044+TTTTCTATTTTGAACGGGCCTAATCTAGCCAAAAG[A/C]AATTACATGCTATAAATATCACTATAAGAAACTAA

S1211176216182-AACCTTTTGGGGTGGGGTGTAGATTTTTCCATCTT[G/T]TATTTTCTATTTGAAGAGCATGTTTGTAGATGGAG

S1221177616308+CTAAAAGGTATTGGGGAGAAGTGCATGATTAGGCA[C/G]GACATGACACAACTTCAGCTTAGCAAGGACATTAC

S1231179128938-ACTTGATGCCTTCTATCCAACCTTATCCCGGAACA[T/A]TAAAAAATCAATAACAAGAGATATGCATGCGTAGA

S1241181092673+TCGACTCCAACATTGGATTATGGTCCTAAACTCTG[T/C]CAGTCACGCCTCATTCAGCATCCCAGAGAATAAGC

S1251182275454+TCTAGCCTTCAAAACCTGAGAATTATGCAGATGTT[G/T]TTTCTGTAGCTGCCGTATTTCATCTTCCATCGAGG

S1261183255636+TATGAGAGATGTCATCTGAAAAGAACTATTGAATG[G/A]AGATGAAAAGTAAAGTAATCGTCTAAAGCTGATAG

S1271185954035+GTAACTGATCCTTTAACCCATTCAACTCAGAAGGG[T/G]CCATACGATACGGAGGAATGGAAATGGGCTGAGTT

S1281186690069+CTCTTTTTGGTACTGGAGGTTTGAAGTACTCTGTC[T/C]GCACCTGGAGTTTGGGCCTACTGTCCGCGTGAAGT

S1291188871548+GGTGATGAGGACTTACCTGCACTAAGACTTATACC[T/C]TGGTTGCGGCAAAAGCACTTTTTTTTTTGTCAAAA

S1301190023913-TTCTTCTTCTTCAGATAGAGGTTTATGTAGATACT[A/G]AAATTTTAACAGATTAAGATAAATCCTAAATTAAA

S1311191262094-TGGATCAATAACCCTTATGTCCACGTCTTTGCTAG[C/T]CATGCTTACCTTGCTCTTTGACCTTGTGTTGTATG

S1321193121021-CTCTTTTGATGATACATAACTTGGCGTTCTGATCT[A/G]CGCCAGCCATGCTGACTGAGAGAATGGTCTTTCTT

S1331194104878-TCGGTAAGAATCTTAAGGGAATCACTAAGCTAGTA[G/C]AACCAAAACGTCACGGTACCACATTTGGACTTGGG

S1341196287148-TAAACTTGTATCAGTTTGCCAACCCAGTAAATAAA[T/C]ATATGTCTTTCCCATTTTAACACCTTAACTTGATG

S1351197543720+AGATGAAAATGACGTTGTAGTAGAGGAAGATGATA[T/C]TGCAGGTACTGGAGATATTATTTCAGAAGATGAAG

S1361199000692+CAGTTAGTGGCTGCTCAAGTATCTAATATTTTCAA[C/G]TGTGACAATACGCACCAGTTATCGAAATCAAAGGA

S1371200571323+CAATTATACATGATGTGCAATGTATGATAACTGAG[A/T]CTGAGATATGATATGTAATGATGAATACGACTGAG

S1381202147101+TGTTTGTTGTTTCTGTTTCTACTTTATATTATCGA[A/T]TACAAATAATACACTAGAAGACAATATTTTTGGTT

S1391204309448-CATGATAGAGACTGAAAGAGTATGCTCTTCCTCCT[T/C]TGTGGATCTTCTGAGGAGGCTGCATAATTTTGCCC

S1401205552119+ACCTTGATAATGCATCTGGGTTGATTCAAGCGGAC[A/G]CAGGCTTAGCGAGTCGACGAAGCCTCGCACACCGA

S1411206653545+TTTGAAGTATTCAGCTAAATATCCATGCAAGATTG[C/T]GGCATGCTATGAAGATAAGGTACTTTGGATTACCG

S1421208104698+ACAACAAATGGCTAACTCAGCTGCTCAATACTACC[A/T]GAACCAGTTCACCAATCAAGGGGATCCCACAGATT

S1431209150810-CGGCATTATTCGTTGGGTACATTCAGTAAACCAGA[T/C]ACACCGTATTTTCTAACACAAGAAGCTGTTTTCCA

S1441210662467-TGGCCTCATAACTACACGATCGCGAGGGAACATAC[G/A]CGAACACATACCTCTACTACTCGACCTCGTTCAGC

S1451212123743-GGTTGACCAGGTCTTAGAGGTCATATTAGTATAGC[T/C]GGTTGTCCCAGTTTAGCTCGATATTAGGGTAGCAA

S1461213745102-AATTCAGTTCAGTTTAGAAATTTCAATAAAAGACA[T/G]GCATGCTTTCAAATCCGAAAACTCAAGTCAAATAA

S1471215163907+CTCGATATGTCAATTATATAACCAAGGACAAGCTC[G/A]TGCAAGTTAAGGAGGAGTGCTAGTGGAAGAACAAA

S1481216797624+GAATGAAAAAATAGTTAATCAATGCTTTCACTTAC[G/A]ATTCATGTTCCATTCCTCGGGGCATGGCATCCTCT

S1491218312295-AAACTTTTAAGGATCCAACCTAAAAACATTACTAA[T/G]AACCTACATTCCATACAAATATTAAATAATAGAAA

S1501221127891+TTCTACTCTAACCTCGTCTATTAGAACCTCAATTT[C/T]TGTATCTACTTCATGCTCTTCTTGGCTATTATCCC

S1512450633+ATCTGACCCATCGGTCAATCCACCCACGATGCCTA[T/C]GTCTGCAAGGTTGACCTTTTTGGCTGCAAATTTCG

S1522936605-AGTCTCCAAATTGACTTGTCCACCCAATATATCAG[C/T]CAAGAGACGTACCTTTCGAGTTTTCCGACGTGGTA

TACTACATCGAATACTGCGGATGTCCCAGATGATC[A/G]TTCTTTTGTGAATTTCTTCAGTGTATGCTCTTTCT

GAGAGAGGAAGACTGATTAGGTGTAGAGAAAGATA[T/C]AGGAAGTGAAGTAGGAGGGTTGGTGAAATAAGTAG

GAGCACAGTTTATGCAACAAATTAAGAGGAACTAT[T/G]AAGGGACCATCACTATCCTAAGAAGAAAGATAGCC

4

YC/T××××—××××

表A.1（续）

序号染色体位置正负链序列

GGCCAAAACTGAATGACCAAGCCTGAATTCTAATT[A/G]AAACAACTGAACTGAATTTAAATCAAAACTAACAC

S157210113165+CTCCAGACTAAGTTTCAATAGTTAATTATGTACCA[A/G]ATCAAATGGTCTGAGGAGCCGCGCCTGCACGGGCC

S158212637986+TTTTGCTGGGTCAGAAACTCTAAGTTCCACCCTTC[A/G]CAATTTTTGGCCATTTTTCTCAATATTTCATTCGG

S159213854934+ACCTTTAAACATGGTTAGATTCTTGAAAGTCTAAG[C/T]TGAAACCAAGGCTGGTTTGGTAGTAGGAAGCCATA

S160215731118+TTGTTCATAAAGGTCCTTGCTTGATTATATCTTAA[A/C]TGTTCCCCTATTCTCGACTATCCCCGATAGCTCGA

S161216703846+ACACCGACAGAAATGTGCTACACAACGACCAATAG[A/G]AAGTTCAAAAACCCCAGCCCGAGAAGAACAGGAAG

S162218365346-TAAGGGTAACAATTGAAGTCCACGTTGAGGGTAAC[C/A]TTTTAAGGAAATCAGGTCAAGCTGACGTGTGGCTA

S163219909410+TATCTCTCATGGAGGCCACTATAGTCAGAGCTTAC[C/T]TGGCTAACGAGTCAAAACTAAGGTTATATTGCCTT

S164222038721+GTATCATTTGTAATTCATCACTTTTTTGTAAGGAC[A/C]CTCTGTGGGCTTTTGTAATCACGTGCAATGTAACC

S165222954985+CCCTAAATGAAAATGTTGCATAATCGATAAAGAGC[T/A]AGACTCCTTACTTGAAGAGAACTTTATCCTAGATA

S166224687385-ATTCTATATGTTTGTATGGTTAAGTGCTTCAAATT[C/A]CAACATGCTTCTGCTGGACATATATTTCATGTTCT

S167225796400+TGATCTTAGGTTGAGCTATGTGCTTGGAAATACCC[A/G]GATATCAATGCCAACCAACCATGAGTTCTATGACG

S168227426888+CTAAAAGAGGATGTTTTGGATGATCCCTCAGATGC[A/G]GCGAGGTTGTTATAGGAGGCCTTTACCCGAATAGG

S169229011893-GACTATGTTATGGGGTCTGACCAGTCAAAGAACTC[T/C]CATATCCAGAAGATGAACGTAGCTGAGATGAAGAT

S170229835254-GTGTGTCCTTAGGAAATGACAAAAATGGATACATT[C/A]TAGGAGTTGGAAAAATTGGGAATACACCCACTCGT

S171231244480-AGAGAAAGCACACTTGTTTGAGGGATTAGTAATAA[A/T]ATCTCACCTTGTGCTTTTACCTCGTATCAATAATA

S172233518648+TAGTTCACAACAGCCCATAAAATAGTTTTCATATC[T/C]AAAACCCAGTATCCATAAAAGATTCAAAGAAATAG

S173234990647+TGAAAAAGTTACCTCAGAATTAATATTAGCACGCT[A/C]TACCGCCCAAGATTAGTCATATATCCGCTTAAGTC

S174236400612-ATCATGAGCGGTTTGCAATTACAGAAACTAGACTC[T/G]GAGCACTACAATATACCAAAAATTTGGATTTAAAT

S175237416318-TATAAGTGGAGCATGATTATTTTGTGAAGTTGACA[G/A]ATTGATTAGCTTTTTTAATTTAATGGTGATATTTT

S176239105905-GAGCTAACCACAAATTTCTAAAAGTTCAGATTGTT[T/G]TTCTTCTGAGAAAACAAAAGGGAAGTCTTGTGATT

S177240504754-ACACGGCAACCAACAATTATCCTAAAGAAACCTAG[C/A]TGAATTATTGACTTAATAAAGAAAACTGATTCAAT

S178241504460-CACTTCATTTTCTCCAGATTGTAGCAGTGAAACAG[C/T]TTGACCGCAATGGATTGCAAGGAAACCGAGAATTC

S179241954634+TGTCTGAATGGTACATTCAGATTGTCTGTCAGTCT[T/G]AGCATGGAATGTAGTGCTGAGATTCACTTATGTTC

S180243431076+TCTTATAATGATCCTTTCTTCAACAATATTCCCAT[C/T]CCTGGTGATCCTTTAATGTATCTCACAACACTTAA

S181245859741-GCATCAGTGAATCCATTCTAGACCTTAGAAACATG[T/C]TGGAATTCTGTCTTTGTGAACCTCTTTCTCAACTC

S182247069679+CCTGATCGTTTGATTTCATTGGAAATTCACCGGAG[A/T]TTTGAAGACCATGGCTGTCGATGACACTTTGCTGG

S183248357443+AATCTCTCGAAACATGATAAGACCATTACATCTCT[A/G]ATTACAACAACAACAACAACCTAGTGAGATCCCAC

S184249898177-GTAGAAAATATGGCATTCACCGTAAGGTGGGCAAT[C/A]CGTATCTCCCCAAACAAGTGGACAAGTGGAGCTAT

S185251046894-TGAACAATGCAGATACAATATACAAATTAGTTACT[T/A]CCATCCTTTTGTATCCATTATGATTGAGTTTTGGT

S186252688146+GCTGCTACTAAACAACATGTCATCGAAACTGGACC[G/A]GATGATCTTTGGAGGAAAGTACGGGCAAGGGAATA

S187253939330-AATAAGAGAATCTTCCACATGAGTAGACGTATAAG[A/C]AGGGGAACTCAAAGAATTCGAGATACGCCCAAATG

/p>