课件套路04 mirna研究四章

CHAPTER4CHAPTER4:THECORETECHNOLOGYOFmiRNA••AmbrosV.••AmbrosV.TheC.elegansheterochronicgenelin-4encodessmallRNAswithantisensecomplementaritytolin-14.[J].Cell,1993,75(5):843.••6••6CLIP-CLIP-::http://zmf.umm.uni-mirSVRPPhastCons::http://zmf.umm.uni-mirSVRPPhastConsCHAPTER4CHAPTER4:THECORETECHNOLOGYOFmiRNA2-seed3.2-seed3.540-460-=540-460-=miRNA miRNAmiR-OncolLett.2017Nov;14(5): miRNA miRNAmiR-OncolLett.2017Nov;14(5): miRNA miRNAmiR- miRNA miRNAmiR- 1miR-RedoxBiol.2018May;15:284-OncolLett.2017Nov;14(5):1miR-RedoxBiol.2018May;15:284-OncolLett.2017Nov;14(5):++miRNA++miRNAOncolLett.2017Nov;14(5):++miRNA++miRNAOncolLett.2017Nov;14(5):+miRNA++miRNA+1<2<3=OncolLett.2017Nov;14(5):+miRNA++miRNA+1<2<3=OncolLett.2017Nov;14(5):CHAPTER4CHAPTER4:THECORETECHNOLOGYOFmiRNAmiRNAEXTRACTIONINmiRNAEXTRACTIONINmiRNAEXTRACTIONIN-2.3.4.miRNAEXTRACTIONIN-2.3.4.miRNAEXTRACTIONINFabbriM,PaonemiRNAEXTRACTIONINFabbriM,PaoneA,CaloreF,etal.MicroRNAsbindtoToll-likereceptorstoinduceprometastaticinflammatoryresponse.ProcNatlAcadSciUSA.2012JulmiRNAEXTRACTIONINMariaEldhetal.ImportanceofRNAisolationmethodsformiRNAEXTRACTIONINMariaEldhetal.ImportanceofRNAisolationmethodsforanalysisofexosomal miRNAEXTRACTIONINMariaEldhetal.ImportanceofRNAisolationmethodsforanalysisofmiRNAEXTRACTIONINMariaEldhetal.ImportanceofRNAisolationmethodsforanalysisofexosomal miRNAEXTRACTIONINAgilentSmallmiRNAEXTRACTIONINAgilentSmallmiRNAEXTRACTIONINReal-timemiR-/Sipkecel-miR-miRNAEXTRACTIONINReal-timemiR-/Sipkecel-miR-miRNAEXTRACTIONINReal-timeOcchipintimiRNAEXTRACTIONINReal-timeOcchipintiG,GiuliettiM,PrincipatoG,choiceofendogenouscontrolsinal.microRNAassessmentsfrombiofluids.Tumour2016Sep;37(9):11657-miRNAEXTRACTIONINVanDeunJ,MestdaghP,R,etmiRNAEXTRACTIONINVanDeunJ,MestdaghP,R,etal.TheJR,KangQ,IK,etal.QuantitativedisparateisolationmethodsforextracellularvesiclesondownstreamRNAprofiling.JExtracellVesicles.2014Sep18;3.stoichiometricanalysisofthemicroRNAcontentofexosomes.ProcNatlAcadSciUSA.2014;111:14888–93.CHAPTER4CHAPTER4:THECORETECHNOLOGYOFmiRNAPaO2:PaO2:HYPOXIARELATIVEHYPOXIARELATIVEHYPOXIARELATIVEHYPOXIARELATIVEHIF-HIF-HYPOXIARELATIVEHYPOXIARELATIVEHIF-1HIF-1α依赖-途径降解氧浓度HIF-FIH-HIF-HIF-HIF-FIH-1HIF-1HIF-1α依赖-途径降解氧浓度HIF-FIH-HIF-HIF-HIF-FIH-1HYPOXIA